Results 161 to 170 of about 46,126 (304)

Compact 9dBEs Enable Efficient and Precise Genome Editing in Mammalian Cells and In Vivo. [PDF]

Adv Sci (Weinh)
As a compact type II‐D system, the Cas9d‐based platform holds great potential for in vivo applications. Through rational engineering, its derived base editors (9dBEs) enable efficient disease modeling while facilitating single‐vector AAV delivery for in vivo genome editing. These miniature tools offer a robust strategy for basic research and biomedical
Xiao Q, Tian Z, Weng L, Xu K, Han D, Wu Z, Li J, Li S, Zhang Q, Zhao X, Li G, Wei Y, Gu Z.   +12 more
europepmc   +2 more sources

MLH1 p.His112Arg Variant Is Associated With Increased Cancer Susceptibility

International Journal of Cancer, EarlyView.
ABSTRACT Lynch syndrome, a prevalent hereditary cancer predisposition, is frequently caused by pathogenic variants in the MutL homolog 1 (MLH1) mismatch repair gene. However, a significant proportion of MLH1 missense variants remain classified as variants of uncertain significance (VUS), creating clinical ambiguity.
Amal Elfatih, Issam Tout, Najwa Ahmad Doha, Tala Abu Arja, Idris Mohammed, Doua Abdelrhaman, Waseem Hasan, Chadi Saad, Hamdi Mbarek, Borbala Mifsud, Sahar Isa Da'as   +10 more
wiley   +1 more source

Determinants of crispr nuclease grna function

, 2023
CRISPR (clustered regularly interspaced palindromic repeats) systems are sought-after genome editing tools for therapeutics and diagnostics. The use of CRISPR systems for gene editing is not without difficulties: the specificity of Cas12a nuclease, a Cas9 alternative, presents a limit in its use for gene editing due to its inability to successfully ...
openaire   +1 more source

Iqcm Is Dispensable for Male Fertility in Mice

iNew Medicine, EarlyView.
Schematic overview of the experimental workflow. Iqcm is highly expressed in mice testis (left). Iqcm−/− mice were generated via CRISPR/Cas9 (second panel). Phenotypic analyses including testis/epididymis histology (H&E), sperm morphology, and litter size revealed no significant differences between Iqcm+/+ and Iqcm−/− mice (third panel).
Zhaojuan Yu, Yuhui Ye, Mingfei Xiang, Zhaolian Wei, Zhen Lu, Jia Peng, Yunxia Cao   +6 more
wiley   +1 more source

Information extraction challenge Gene Regulation Network in Arabidopsis thaliana (GRNA)

, 2015
Information extraction challenge Gene Regulation Network in Arabidopsis thaliana (GRNA).
Chaix, Estelle, Bossy, Robert, Bessieres, Philippe, Fatihi, Abdelhak, Zweigenbaum, Pierre, Nédellec, Claire, Lepiniec, Loic, Dubreucq, Bertrand, Deleger, Louise, Valsamou, Dialekti   +9 more
core   +1 more source

Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics

Interdisciplinary Medicine, EarlyView.
This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li, Han Li, Bojie Chen, Ling Jiang, Keyu Long, Jiaqi Lang, Xingxiang Pu, Erhu Xiong   +7 more
wiley   +1 more source

CRISPRware: a software package for contextual gRNA library design

BMC Genomics
Abstract We present CRISPRware, an efficient method for generating guide RNA (gRNA) libraries against transcribed, translated, and noncoding regions. CRISPRware leverages next-generation sequencing data to design context-specific gRNAs and can account for genetic variation, which allows allele-specific guide design on a genome-wide scale.
Eric Malekos, Christy Montano, Susan Carpenter   +2 more
openaire   +2 more sources

The epigenetic landscape of plant defense: Unlocking nature's toolkit for new plant protection

New Plant Protection, EarlyView.
This review examines how epigenetic mechanisms regulate plant immune gene activation, repression, and priming, then links these natural regulatory layers to emerging epigenetic editing, discussing their potential for precise, heritable crop protection and the challenges.
Xuexia Liu, Zhenhui Zhong
wiley   +1 more source

Heritable gene-targeting with gRNA/Cas9 in rats [PDF]

Cell Research, 2013
Xinli, Hu, Nannan, Chang, Xuelian, Wang, Fengyun, Zhou, Xiaohai, Zhou, Xiaojun, Zhu, Jing-Wei, Xiong   +6 more
openaire   +2 more sources

Transcriptomics Unveil Dsx1 as a Critical Regulator in Sexual Dimorphism of Crustaceans

Integrative Zoology, EarlyView.
Sexually dimorphic traits are involved in reproductive competition and are shaped by sex‐biased gene expression. This study identifies Dsx1 as a key male‐biased gene in Morinoia aosen and demonstrates through RNA interference that its disruption feminizes male‐specific T3 leg structures.
Yan Tong, Wenpei Xin, Zeyu Liu, Yami Zheng, Mengyi Huang, Hongguang Liu, Decai Lu, Wei Peng, Zhonge Hou   +8 more
wiley   +1 more source