Results 171 to 180 of about 44,236 (298)

Transgenic selection and underlying mechanisms in apicomplexan parasites

The FEBS Journal, EarlyView.
Transgenic selection markers have driven genetic engineering in apicomplexans, enabling precise, iterative experiments. This review discusses mechanistic details of drug selection markers, strategies for marker recycling, and practical considerations for several clinically relevant parasites.
Swaroop Peddiraju, Rakhee Lohia, Nishith Gupta   +2 more
wiley   +1 more source

TRIM32 controls timely cell cycle exit in muscular differentiation through downregulation of c‐Myc mRNA

The FEBS Journal, EarlyView.
Trim32 regulates the transition from proliferation to differentiation in C2C12 cells after myogenic induction. We found that, at the onset of differentiation, Trim32 destabilizes c‐Myc mRNA, promotes cell cycle exit, and enables normal myotube formation.
Lu Xiong, Elisa Lazzari, Sabrina Pacor, Simeone Dal Monego, Erica Piovesan, Danilo Licastro, Germana Meroni   +6 more
wiley   +1 more source

Turnip Mosaic Virus-Based gRNA Delivery System for Plant Genome Editing. [PDF]

Plant Direct
Khwanbua E, Lappe RR, Bierl AA, Whitham SA.   +3 more
europepmc   +1 more source

Circadian rhythms of metabolite abundance in Drosophila are largely driven by time of feeding

The FEBS Journal, EarlyView.
Circadian clocks exist in the brain and peripheral tissues such as the Drosophila fat body. Single‐nuclei transcriptomics reveal that the fat body clock generates time‐of‐day differences in metabolic gene transcription, which may also be indirectly controlled by brain clocks.
Sumit Saurabh, Charlene Y. P. Guerrero, Madelyn R. Cusick, Amanda J. Samaras, Taylor M. Stephenson, Sebastian L. Kirkpatrick, Gregory J. Matthews, Daniel J. Cavanaugh   +7 more
wiley   +1 more source

Clonal Analysis in <i>Drosophila</i> Tissues With an Enhanced MAGIC Transgenesis Method. [PDF]

Bio Protoc
Shen Y, Han C.
europepmc   +1 more source

Contribution of endosomal proteins EHD1 and EHD4 to the HIV‐1 replication cycle

The FEBS Journal, EarlyView.
EHD1 and EHD4, two key trafficking proteins, regulate multiple stages of HIV‐1 infection: In producer cells, they help produce fully infectious virus particles, regulate Envelope protein incorporation in a cell‐type‐dependent manner, and limit the accumulation of antiviral IFITM proteins in virions.
Grégory Pacini, Konstantinos Poalas, Margaux Versapuech, Clarisse Berlioz‐Torrent, Katy Janvier   +4 more
wiley   +1 more source

Dual-gRNA CRISPR/Cas9 Deletion of CsDMR6 in Sweet Orange Supported by Improved In Vitro Regeneration. [PDF]

Plants (Basel)
Sopalda S, Vergara R, Muñoz M, Aguirre C, Muñoz C, Prieto H.   +5 more
europepmc   +1 more source

The trimeric Shu complex in C. elegans is an ATPase that remodels RAD51 filaments in the homologous recombination‐associated DNA damage response

The FEBS Journal, EarlyView.
The C. elegans Shu complex (RFS‐1/RIP‐1/SWS‐1) is a DNA‐dependent ATPase that regulates RAD‐51 filaments during homologous recombination. The trimer preferentially binds 5′‐exposed DNA, remodels RAD‐51 filaments via ATP hydrolysis, and stabilizes filaments through ATP binding.
Sam Siu Hang Chu, Guangxin Xing, Kichang Paik, Anton Gartner, Hong Ling   +4 more
wiley   +1 more source

Phosphorylation of BigH1 regulates its expression pattern and promotes embryonic development

The FEBS Journal, EarlyView.
Phosphorylation is a key regulator of the linker histone variant BigH1 during Drosophila embryogenesis. We reveal that N‐terminal phosphorylation promotes the timely removal of BigH1 from chromatin. In contrast, C‐terminal phosphorylation supports early nuclear divisions and embryonic viability.
Ramóna Pék, Aladár Pettkó‐Szandtner, Anikó Szabó, Balázs Vedelek, Bence György Gombás, Zoltán Villányi, Péter Vilmos, Imre Miklós Boros, László Henn, Gyula Timinszky   +9 more
wiley   +1 more source

abCRISPR: deep learning-based design of abasic gRNA sequences for specific CRISPR-Cas9 genome editing. [PDF]

Bioinformatics
Kim GD, Gu D, Park M, Chi SW.
europepmc   +1 more source