Results 191 to 200 of about 44,236 (298)

Towards efficient perturbation for the noncoding genome. [PDF]

Nat Commun
Zhang H, Luo S, Wang X, Lin L, Liang R, Zhong C, Zhang Y, Zhao W, Chen Z, Liu X, Gao Y, Chen F, Sun N, Huang J, Fei T.   +14 more
europepmc   +1 more source

gRNA

Farumashia, 2018
openaire   +1 more source

microRNA‐mediated control of cell fate specification and patterning in Marchantia polymorpha

New Phytologist, EarlyView.
The gemma developmental program in Marchantia polymorpha is coordinated by MpMIR166‐mediated repression of MpC3HDZ and auxin homeostasis. Summary MicroRNAs (miRNAs) are small non‐coding RNA molecules essential for growth and development in eukaryotes. In plants, the master gene DICER‐LIKE 1 (DCL1) catalyzes the biogenesis of miRNAs by processing double‐
Adolfo Aguilar‐Cruz, Eduardo Flores‐Sandoval, Ye Xu, Yu Yu, Ximena Gutiérrez‐Ramos, Cristian Y. González‐Rangel, Omar Oltehua‐López, Ana E. Dorantes‐Acosta, Joshua T. Trujillo, Hirotaka Kato, Kimitsune Ishizaki, Rebecca A. Mosher, Liam Dolan, Xuemei Chen, Daniel Grimanelli, Jim Haseloff, John L. Bowman, Mario A. Arteaga‐Vazquez   +17 more
wiley   +1 more source

Adenovirus Vector-Mediated In Vivo Knock-in Treatment of Neonatal Phenylketonuria Mice Using Terminally Cleaved Donor DNA. [PDF]

J Gene Med
Yamaji M, Tabata H, Nakamura M, Fuse R, Okada T, Ohba SI, Ohishi T, Kawada M, Saito I, Sato S, Nakanishi T.   +10 more
europepmc   +1 more source

Auxin‐related regulation of barley floral organ fate: genetic and phenotypic insights from tweaky spike mutants

New Phytologist, EarlyView.
Inflorescence/floret phenotypes of tweaky spike (tw) barley (Hordeum vulgare) mutants and their respective WT backgrounds. Summary Mutant‐based research is a cornerstone for elucidating gene function in barley (Hordeum vulgare) and other cereals. In this study, we exploited a unique collection of barley tweaky spike (tw) mutants to identify the locus ...
Vėjūnė Pukenytė, Bernadeta Kaminskaitė‐Mačė, Miglė Remeikytė, Jurga Jankauskienė, Julius Juodakis, Sigita Jurkonienė, Algė Leistrumaitė, Ljudmilla Timofejeva, Virginija Vaitkūnienė, Justas Stonkus, Raimondas Šiukšta   +10 more
wiley   +1 more source

UNCOVERseq enables sensitive and controlled gene editing off-target nomination across CRISPR-Cas modalities and systems. [PDF]

Nat Commun
Kinney KJ, Jia K, Zhang H, Schmaljohn E, Osborne T, Thommandru B, Murugan K, Sánchez-Peña A, West S, Chen S, Codipilly R, Sturgeon M, Turk R, McNeill MS, Behlke M, Jacobi A, Cromer MK, Rettig G, Kurgan GL.   +18 more
europepmc   +1 more source

Knocking Out Two Polyphenol Oxidase Genes Significantly Improves Recombinant Protein Purification in Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
ABSTRACT Efficient purification remains a key technical challenge affecting the recovery efficiency of recombinant proteins in plant‐based systems. Complex metabolites, particularly polyphenols, often cause recombinant protein aggregation during purification.
Hai‐Ping Diao, Hui‐Xin Meng, Xue‐Jiao Xu, Zeng‐Lin Zhang, Jian‐Feng Zhang, Yong‐Feng Guo, Inhwan Hwang, Shi‐Jian Song   +7 more
wiley   +1 more source

TRPV4-mediated calcium influx contributes temperature-dependent SARS-CoV-2 replication. [PDF]

PLoS Pathog
Kobayashi M, Abe H, Sugihara H, Kobayashi N, Omori S, Yamada Y, Ichinohe T.   +6 more
europepmc   +1 more source

In planta Processing of the SpCas9-gRNA Complex

In planta Processing of the SpCas9-gRNA Complex
2018年度
openaire   +1 more source

Modeling NDD-Associated NLGN2 Depletion Using CRISPR/Cas13 Reveals Exaggerated Process Elongation Mediated by the CCDC88A-G Protein-ELMO Axis. [PDF]

Int J Mol Sci
Yako H, Takahashi M, Tada S, Waragai M, Miyamoto Y, Yamauchi J.   +5 more
europepmc   +1 more source