Results 201 to 210 of about 44,236 (298)

A Robust Framework for Maize Elite Line Genome Editing Through Enhanced HI‐Edit via LbCas12a Activity Optimization

Plant Biotechnology Journal, EarlyView.
ABSTRACT Haploid induction coupled with genome editing (HI‐Edit) enables direct modification of commercial crop varieties, bypassing the need for trait introgression or direct transformation of elite lines with CRISPR machinery. However, its widespread application has been constrained by low haploid editing rates (HER), the proportion of haploids ...
Dawei Liang, Huanhuan Guo, Juan Wei, Fugui Zhu, Yuguo Zhang, Julie Green, Yun Ji, Huaibing Jin, Xiujuan Zhang, Huaping Gui, Hongmei Dan, Yubo Liu, Yu Zhang, Han Wang, Yutong Jiang, Lizhao Geng, Jian Lv, Wen Cai, Weibin Song, Timothy Kelliher, Xi Chen, Rachel Egger   +21 more
wiley   +1 more source

CRISPR-mediated excision of HTLV-1 reduces proviral loads in PBMCs from HAM/TSP patients. [PDF]

J Neurovirol
Brancazio S, Khalili K, Jacobson S, Kaminski R.   +3 more
europepmc   +1 more source

RNA‐Guided Engineering of the Chloroplast Genome Enabled by Plastid‐Expressed Guide RNAs

Plant Biotechnology Journal, EarlyView.
ABSTRACT Our goal is to develop RNA‐guided engineering of the chloroplast genome using the CRISPR/Cas9 system. We designed chloroplast minigenes to obtain properly sized single guide RNAs (sgRNAs) in tobacco chloroplasts. The sgRNA 5′ end is defined by transcription from an rRNA operon promoter, and its 3′ end by processing a downstream tRNA (trnG) or ...
Malihe Mirzaee, Corinne Best, Evelyn V. Wachowski, Pal Maliga   +3 more
wiley   +1 more source

On-target and off-target activities of CRISPR therapeutics across scales. [PDF]

Trends Biotechnol
Magdy M, Gibson JR, Dhungana A, Herring-Nicholas A, Cottle RN, Josephs EA.   +5 more
europepmc   +1 more source

A Dual-gRNA CRISPR/Cas9 System for Efficient Generation of Large Fragment Deletions in Poplar. [PDF]

Bio Protoc
Yang G, Yu Y, Vulavala VKR, Dwivedi N, Liu CJ.   +4 more
europepmc   +1 more source

CRISPR/Cas9‐Mediated Knockout of LbSP1 and LbSP5G1 Reveals Efficient Customization of Shoot Architecture in Black Goji Berry

Plant Biotechnology Journal, EarlyView.
ABSTRACT Goji is recognized as a nutritionally rich superfruit with medicinal and industrial importance. However, its inherently indeterminate growth habit causes asynchronous flowering and uneven fruit ripening, resulting in harvesting inefficiency with high labour costs and low fruit yield.
Fazal Rehman, Huanfang Liu, Yun Ma, Shaohua Zeng, Yan Wu, Yuan Zong, Chao Yang, Ying Wang   +7 more
wiley   +1 more source

Identification of a Susceptibility Gene for Xanthomonas oryzae pv. oryzae in Vietnamese Elite Rice (Oryza sativa L.) Cultivar Bacthom 7

Plant Breeding, EarlyView.
ABSTRACT Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of bacterial leaf blight (BLB) disease in rice, exerting a detrimental impact on the yield of various rice cultivars in Vietnam, notably the Bacthom 7 (BT7) variety. Xoo possesses transcription activator‐like effectors (TALEs) which modulate the expression of host genes by specifically ...
Nguyen Duy Phuong, Dong Huy Gioi, Cao Le Quyen, Nguyen Xuan Hung, Ha Hai Yen, Bui Thi Thu Huong, Sebastien Cunnac, Tran Dang Xuan, Pham Xuan Hoi   +8 more
wiley   +1 more source

CasPINS: an integrated web-based platform for CRISPR/TALEN gRNA design, primer generation, and indel decomposition analysis. [PDF]

Bioinform Adv
Dasgupta R, Das K.
europepmc   +1 more source

A Repertoire of Major Genes From Crop Wild Relatives for Breeding Disease‐Resistant Wheat, Rice, Maize, Soybean and Cotton Crops

Plant Breeding, EarlyView.
ABSTRACT Global food demand is predicted to rise anywhere from 59% to 98% by 2050 because of increasing population. However, the continued depletion of natural resources and increasing biotic and abiotic stresses will continue to pose significant threats to global food security in coming years.
Memoona Khalid, Hafiz Mamoon Rehman, Tsz‐Yan Cheung, Shakeel Ahmed, Ting Fung Chan, Hon‐Ming Lam   +5 more
wiley   +1 more source

A self-iterative orthogonal base-editing platform enables multiplex N-to-N diversification and genome-scale functional screening in Escherichia coli. [PDF]

Nucleic Acids Res
Fan X, Liang L, Wang H, Liu G, Tan H, Zhang F, Liu L, Liu R.   +7 more
europepmc   +1 more source