Results 221 to 230 of about 44,236 (298)

CRISPR/Cas9‐mediated Genome‐editing Reveals 10 Testis‐enriched Genes and One Non‐testis‐enriched Gene are Dispensable for Male Fecundity in Mice

Andrology, Volume 14, Issue 7, Page 2150-2159, October 2026.
ABSTRACT Background More than 1000 genes have been identified as predominantly expressed in the human testis. Advances in gene editing technologies have enabled the rapid and efficient generation of genetically engineered mice. This approach facilitates the screening of genes essential for spermatogenesis by analyzing knockout mouse models.
Yumiao Qiu, Keisuke Shimada, Masahito Ikawa   +2 more
wiley   +1 more source

CRISPR/Cas9-based repair of a heterozygous HNF1A mutation in patient-derived hiPSCs. [PDF]

Hum Genet
Skoczek D, Hohendorff J, Malecki MT, Roig-Merino A, Bak RO, Kachamakova-Trojanowska N.   +5 more
europepmc   +1 more source

Mutual antagonism between auxin and diacylglycerol acyltransferase 2 regulates high‐temperature‐induced hypocotyl elongation

New Phytologist, Volume 252, Issue 1, Page 222-240, October 2026.
Proposed model for auxin–DGAT2 crosstalk regulating high‐temperature‐induced hypocotyl elongation. Summary Cell elongation is a fundamental process in plant growth and development, and it is significantly affected by temperature changes. While the signaling mechanisms driving high‐temperature‐induced cell elongation have been extensively studied, the ...
Mohinur Khamzaaliyeva, Punita Lalchand, Didier‐Deschamps Ashley, Xue Pan   +3 more
wiley   +1 more source

The combination of morphogenic regulators BABY BOOM and GRF‐GIF improves maize transformation efficiency and promotes leaf regeneration

New Phytologist, Volume 252, Issue 1, Page 260-275, October 2026.
Maize regeneration via somatic embryogenesis by ectopic expression of morphogenic factors TaGRF4‐TaGIF1 and ZmBBM. Summary Transformation is an indispensable tool for plant genetics and functional genomics. Although stable transformation in maize is no longer a major obstacle, there remains a need for accessible and efficient methods for academic ...
Zongliang Chen, Jiaqi Zhou, Mary Galli, Sessen Daniel Iohannes, Theresa Clark, Juan M. Debernardi, Jorge Dubcovsky, David Jackson, Andrea Gallavotti   +8 more
wiley   +1 more source

Efficient CRISPR-Cas9 delivery and transgene-free multiplex genome editing in plants using cymbidium mosaic virus-derived vectors. [PDF]

Plant J
Huang YW, Hu CC, Cho YH, Tsai CH, Lin NS, Hsu YH, Dinesh-Kumar SP.   +6 more
europepmc   +1 more source

Perivascular Matrix Densification Dysregulates Angiogenesis and Activates Pro‐Inflammatory Endothelial Cells

Advanced Science, Volume 13, Issue 49, 3 September 2026.
Perivascular matrix densification promotes the emergence of aberrant endothelial tip cells (ATECs) that invade and persist within fibrotic microenvironments. Using in vivo lineage tracing and a human microvessel model, this study shows that fibrous matrix cues destabilize VE‐cadherin–mediated junctions to gate TGF‐β signaling, inducing a pro ...
Jingyi Xia, William Y. Wang, Kyle A. Jacobs, Kairav Maniar, Daphne Lin, Evan H. Jarman, Daniel L. Matera, Kristen Loesel, Christopher D. Davidson, Harrison L. Hiraki, Xiaotian Tan, Eve H. Shikanov, Robert N. Kent, Carole Parent, Xudong Fan, Ariella Shikanov, Matthew L. Kutys, Brendon M. Baker   +17 more
wiley   +1 more source

A high-throughput, streamlined cloning protocol to generate guide RNAs for CRISPR activation. [PDF]

Front Genome Ed
Zhu S, Tamez González AA, Alokda A, Van Raamsdonk JM.   +3 more
europepmc   +1 more source

Cross Kingdom Metabolic Engineering Paradigm Elevating Sustainable Protein Production

Advanced Science, Volume 13, Issue 52, 18 September 2026.
ABSTRACT Confronting the dual crisis of escalating global protein demand and unsustainable agriculture necessitates transformative solutions. Here, we pioneer evolutionary insights from maize nitrogen optimization via asparagine synthetase (ASNS) to rewire metabolism in Pichia pastoris.
Yuanyuan Du, Changyu Pi, Kai Hong, Le Gao, Xin Wu   +4 more
wiley   +1 more source

Upper bound on the mutational burden imposed by a CRISPR-Cas9 gene-drive element. [PDF]

G3 (Bethesda)
Overton MS, Guy SE, Chen X, Martsul A, Carolino K, Akbari OS, Meyer JR, Kryazhimskiy S.   +7 more
europepmc   +1 more source

Ultrasound‐Actuated Gene Editing in Human Kidney Organoids

Advanced Science, Volume 13, Issue 49, 3 September 2026.
Peptide‐stabilized nanoemulsions enable spatially controlled, ultrasound‐triggered delivery of gene‐editing proteins deep within human kidney organoid tissues. Focused acoustic pulses drive droplet vaporization, propelling cargo into target renal cells while preserving native tissue microarchitecture. This non‐viral platform improves both the depth and
Michael A. Miller, Nicole Vo, Utkarsh, Ivan Sokirniy, Justin Pritchard, Benjamin S. Freedman, Scott H. Medina   +6 more
wiley   +1 more source