Results 121 to 130 of about 44,236 (298)

A Method to Convert mRNA into a Guide RNA (gRNA) Library without Requiring Previous Bioinformatics Knowledge of the Organism

Bio-Protocol, 2017
While the diversity of species represents a diversity of special biological abilities, many of the genes that encode those special abilities in a variety of species are untouched, leaving an untapped gold mine of genetic information; however, despite ...
Hiroshi Arakawa
doaj   +1 more source

List of gRNA cassettes used in this study for multiplex gene activation by CRISPR-dCas9.

, 2021
Sequences of gRNA cassette fragment (HH–20 bp specific determinant sequences–structural gRNA–HDV) with the addition of EcoRI and KpnI restriction sites for pOtAOX-gRNA plasmids construction in this study. EcoRI (gaattc) and KpnI (ggtacc) sequences are in
Sutipa Tanapongpipat (3126720), Aekkachai Puseenam (2902434), Niran Roongsawang (2902437), Worarat Kruasuwan (11495350)   +3 more
core   +1 more source

Dual‐line Genome‐scale CRISPR Screening Enables Robust Target Gene Discovery

Advanced Science, EarlyView.
A species‐optimized CRISPR platform integrates efficient piggyBac delivery, genome‐scale sgRNA libraries, and parallel screening in two independently engineered Bactrocera dorsalis Cas9 cell lines. Cross‐line consensus analysis filters line‐specific effects, enriches candidates with reproducible in vivo phenotypes, and reveals conserved, species ...
Ziniu Li, Jiao Qiao, Qiuyuan Zhang, Peipei Liu, Chenjun Zheng, Qi Zhou, Mengqi Ji, Zhihui Zhu, Xiaoxue Li, Hongyu Zhang   +9 more
wiley   +1 more source

Asymmetric Evolution of Antennal Cell Types Underlies a Derived Ammonia‐Sensing Logic for Ecological Adaptation in Bactrocera dorsalis

Advanced Science, EarlyView.
Comparative antennal single‐nucleus transcriptomics reveals cell‐type‐dependent transcriptomic divergence in Bactrocera dorsalis, with similar structural cells but divergent sensory neurons. This neuronal diversification is associated with a distinct ammonia‐sensing pathway, linking female attraction to bird‐dropping‐associated cues with nutritional ...
Wei Liu, Shuai Zhang, Jie Zhang, Zhen Tian, Yuanbo Xue, Ling Yang, Jun Xu, Guirong Wang   +7 more
wiley   +1 more source

MOESM12 of Gene repression via multiplex gRNA strategy in Y. lipolytica

, 2018
Additional file 12: Fig. S6. Photos and its corresponding microscopic images of the interfered strains by dCas9-Multi system. Four related plasmids were transformed into strain VioABE-K8GFP respectively to form four strains: control (means no gRNA ...
Hong Liu (50694), Ying-Xiu Cao (5126522), Ying-Jin Yuan (139604), Jin-lai Zhang (5126519), Duo Liu (758674), Bing-Zhi Li (429468), Yang-Zi Peng (5126516), Chun Li (57810)   +7 more
core   +1 more source

Meetodite võrdlus gRNA efektiivsuse hindamiseks genoomi muundamisel CRISPR/Cas9 tehnoloogiat kasutades [PDF]

, 2020
CRISPR/Cas9 eksperimendi efektiivsust mõjutab gRNA efektiivsus, mis juhib Cas9 valgu sihtmärkjärjestuseni, ning seetõttu on oluline hinnata disainitud gRNA-de efektiivsust genoomi muundamisel.
Kreevan, Rita
core  

Herbivory‐Induced Stomatal Opening Triggers the Dispersal of an Invasive Insect Herbivore

Advanced Science, EarlyView.
The invasive leafminer Phthorimaea absoluta uniquely manipulates plant defenses by inducing stomatal opening in tomato. This response, mediated by the stomatal regulator IQM1, enhances the release of the repellent volatile benzaldehyde. This volatile triggers the dispersal of adult moths, facilitates colonization of new hosts, and leads to rapid ...
Limin Chen, Xiaowei Li, Yaru Wang, Farman Ullah, Shuxing Zhou, Lemeng Dong, Weizhong He, Harro J. Bouwmeester, Youming Hou, Ian T. Baldwin, Yaobin Lu   +10 more
wiley   +1 more source

Targeted Knockout of CYP79A1 Reduces Cyanogenic Potential in Grain Sorghum. [PDF]

Plant Biotechnol J
Plant Biotechnology Journal, EarlyView.
Groover ED, Shen J, Aregawi K, Li S, Schwartz S, Staskawicz BJ, Lemaux PG, Savage DF.   +7 more
europepmc   +2 more sources

Rev- and CRM1-dependent “burst” HIV-1 gRNA nuclear export.

, 2016
(A) Select images from time lapse imaging showing MS2-YFP-tagged RRE-gRNAs and Gag-CFP subcellular distribution over 27.5 hours. “MERGE” shows Gag-CFP (pseudocolored red) and MS2-YFP tagged RRE-gRNA (green) with yellow arrowheads highlighting co ...
Nathan M. Sherer (86591), Jordan T. Becker (3174756), Paul Ahlquist (78214), Chad M. Swanson (195721), Ginger M. Pocock (3174759)   +4 more
core   +1 more source

Uncoupling Type I Interferon Benefits From Inflammatory Toxicity: Transformer‐Prioritized Precision Agonists for Potent and Safer Cancer Immunotherapy

Advanced Science, EarlyView.
A Transformer‐based AI framework, DLINP, screens millions of compounds to identify Co68, a cobalt‐pincer organometallic complex that biases TLR4‐MD2 signaling toward antitumor interferon activation while suppressing inflammatory toxicity through an early TLR4‐SYK‐STAT1 axis.
Xuefei Guo, Yang Zhao, Xianle Rong, Xingyu Chen, Xiao Wang, Tianyi Liu, Yunfei Xie, Yushu Zou, Pingsen Zhao, Qiang Liu, Fuping You   +10 more
wiley   +1 more source