Results 121 to 130 of about 46,126 (304)

Table3_gRNA-SeqRET: a universal tool for targeted and genome-scale gRNA design and sequence extraction for prokaryotes and eukaryotes.XLSX

, 2023
High-throughput genetic screening is frequently employed to rapidly associate gene with phenotype and establish sequence-function relationships. With the advent of CRISPR technology, and the ability to functionally interrogate previously genetically ...
Jan-Fang Cheng (48619), Lisa Simirenko (74525), Lisa Simirenko, Ian Blaby, Ian Blaby (8204187), Jan-Fang Cheng   +5 more
core   +1 more source

NS1‐Induced PARP1 Phase Transitions Prolongs Host Transcriptional Pausing to Promote Influenza A Virus Replication

Advanced Science, EarlyView.
After IAV infection, NS1 induces PARP1 phase transition, reducing molecular mobility in condensates, prolonging host transcriptional pause, aiding RdRp cap snatching, and promoting viral transcription. ABSTRACT Influenza A virus (IAV) commandeers host transcriptional machinery to replicate, but mechanisms remain elusive.
Shufeng Ma, Changyuan Kang, Xixian Liu, Lichao Yang, Mengyi Li, Yuting Ye, Jianfang Zhang, Yingjun Li, Guanghui Jin, Yang Liu, Xiaohong Chen, Kexin Wang, Zifeng Yang   +12 more
wiley   +1 more source

Individual gRNA sequences used for single gene lentiCRISPR targeting.

, 2022
The indicated gRNA sequences were selected based on their enrichment or depletion in CRISPR screens for ACE2 abundance. (XLSX)
Andrew W. Tai (10149208), Vi T. Tang (8356986), Emily J. Sherman (10053054), Cristen J. Willer (7800014), Jonathan Z. Sexton (2263105), Christiane E. Wobus (7610555), Kyle Leix (12174683), Taslima G. Khan (12174680), Sarah E. Graham (10053057), Carmen Mirabelli (152454), Brian T. Emmer (8356980), Andrew A. Kennedy (2450572)   +11 more
core   +1 more source

Integration of the D‐Genome Reshapes Gene Transcription, Chromatin Architecture, and Metabolome of Allohexaploid Wheat, Leading to Enhanced Environmental Adaptability

Advanced Science, EarlyView.
Integration of the D‐genome reshapes gene transcription, chromatin architecture, and the metabolome of hexaploid wheat, leading to enhanced adaptability to salt, UV‐B, and alkali stress. ABSTRACT The hybridization‐led integration of the D‐genome into domesticated tetraploid wheat gave rise to allohexaploid wheat, the most cultivated wheat globally ...
Yanyan Liu, Tao Zhu, Ziru Zhou, Wei Chen, Chao He, Xinkai Zhou, Xin Wang, Chuanye Chen, Bo Yang, Jiaqi Wei, Caixia Lan, Mengmeng Liu, Handong Su, Qiang Li, Xin Hu, Siteng Bi, Weizhi Ouyang, Xingwang Li, Hailiang Mao, Masahiro Kishi, Kerstin Kaufmann, Alisdair R. Fernie, Dijun Chen, Wenhao Yan   +23 more
wiley   +1 more source

Developmentally Guided Differentiation of Ventral Midbrain Dopaminergic Cells Validated in Proteopathy‐Based Parkinson's Disease Models

Advanced Science, EarlyView.
A developmentally guided protocol reproducibly specifies EN1‐positive ventral midbrain dopaminergic (vmDA) progenitors from human pluripotent stem cells and yields functional vmDA neurons. Optogenetic induction of α‐synuclein aggregation elicits selective degeneration of TH‐positive neurons in 2D cultures and 3D neurospheroids, while transplanted ...
Heechang Moon, Heejeong Kim, Elliot H. Lee, Hanseul Kim, Ji Hun Kim, Huisu Jeong, Jiwon Cheon, Ga Ram Jeong, Byoung Dae Lee, Jae‐Won Cho, Soo‐Jin Oh, Yunjong Lee, Yohan Oh   +12 more
wiley   +1 more source

gRNA generated containing HH and HDV ribozyme is efficient at inducing mutagenesis of its target site.

, 2016
(A) mCherryHH-gRNA-HDV construct used to generate gRNA. H2a mCherry is used to label cells expressing gRNA and MALAT is used to stabilize the RNA after cleavage by HH and HDV ribozyme.
Ashley Shu Mei Ng (493205), Raymond Teck Ho Lee (493203), Philip W. Ingham (3342965)   +2 more
core   +1 more source

Blue Light Receptor PHR2‐Mediated Spectrum Antiviral Immunity

Advanced Science, EarlyView.
Blue light suppresses RBSDV through the blue‐light receptor ZmPHR2, which interacts with ZmISP in chloroplasts to trigger ROS burst and antiviral defense. The viral effector P6 enhances ZmPHR2–ZmISP interaction, alters their distribution, and suppresses ROS.
Runyi Chen, Yuxin Tai, Xinyu Wang, Chunhua Mu, Xia Liu, Gongjian Li, Jiayi Liu, Xin Lu, Duo Shi, Zhiqiang Zhou, Jienan Han, Zhuanfang Hao, Mingshun Li, Degui Zhang, Hongjun Yong, Zhennan Xu, Jianfeng Weng, Xinhai Li   +17 more
wiley   +1 more source

Development of Strand-Specific Real-Time RT-PCR to Distinguish Viral RNAs during Newcastle Disease Virus Infection

The Scientific World Journal, 2014
Newcastle disease virus (NDV) causes large losses in the global fowl industry. To better understand NDV replication and transcription cycle, quantitative detection methods for distinguishing NDV genomic RNA (gRNA), antigenomic RNA (cRNA), and messenger ...
Xusheng Qiu, Yang Yu, Shengqing Yu, Yuan Zhan, Nana Wei, Cuiping Song, Yingjie Sun, Lei Tan, Chan Ding   +8 more
doaj   +1 more source

A Method to Convert mRNA into a Guide RNA (gRNA) Library without Requiring Previous Bioinformatics Knowledge of the Organism

Bio-Protocol, 2017
While the diversity of species represents a diversity of special biological abilities, many of the genes that encode those special abilities in a variety of species are untouched, leaving an untapped gold mine of genetic information; however, despite ...
Hiroshi Arakawa
doaj   +1 more source

Enhanced multiplex editing using gRNA arrays.

, 2018
(a) Diagram depicting the plasmid vectors transfected into HEK293T cells to compare gene editing by multiplexing 10 standard U6-gRNA plasmids and a 10 gRNA array.
Kai Hui (5753966), Natalie K. Wolf (5753951), Walker S. Lahr (5753954), Mitchell G. Kluesner (5753960), Samantha Lee (5753963), Morito Kurata (154677), Madison T. Weg (5753957), Masano Shiraiwa (5753969), Branden S. Moriarity (559376), Beau R. Webber (5753972)   +9 more
core   +1 more source