Results 81 to 90 of about 44,236 (298)
Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger. [PDF]
PLoS ONE, 2018 As a powerful tool for fast and precise genome editing, the CRISPR/Cas9 system has been applied in filamentous fungi to improve the efficiency of genome alteration.
Letian Song +4 more
doaj +1 more source
Intronic gRNAs for the Construction of Minimal Gene Drive Systems
Frontiers in Bioengineering and Biotechnology, 2022 Gene drives are promising tools for the genetic control of insect vector or pest populations. CRISPR-based gene drives are generally highly complex synthetic constructs consisting of multiple transgenes and their respective regulatory elements. This complicates the generation of new gene drives and the testing of the behavior of their constituent ...
Nash, A +4 more
openaire +4 more sources
FEBS Open Bio, EarlyView.The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
Structure and use of ribozyme to generate gRNA.
, 2016 (A) Sequence of HH ribozyme. Map of HH-gRNA. First 6 bases (grey text) of the ribozyme are complementary to the first six bases of the gRNA (red text) and cleavage occurs at the 5’ end of the gRNA (arrow). (B) Denaturing PAGE gel of IVT RNA.
Ashley Shu Mei Ng (493205) +2 more
core +1 more source
Enhanced multiplex editing using gRNA arrays.
, 2018 (a) Diagram depicting the plasmid vectors transfected into HEK293T cells to compare gene editing by multiplexing 10 standard U6-gRNA plasmids and a 10 gRNA array.
Kai Hui (5753966) +9 more
core +1 more source
Advanced Healthcare Materials, EarlyView.Mitochondria‐targeted nanotherapies emerge as a promising strategy for combating aging‐associated neurodegenerative disorders (NDs) by restoring mitochondrial function, reducing oxidative stress, and improving neuronal survival. Recent advances in nanotechnology, therapeutic delivery, and translational research are highlighted, providing insights into ...
Dnyandev G. Gadhave +8 more
wiley +1 more source
mSphere, 2018 Cryptococcus neoformans and related species are encapsulated basidiomycetous fungi that cause meningoencephalitis in individuals with immune deficiency.
Ping Wang
doaj +1 more source
Advanced Science, EarlyView.In this study, we unveil a thymus‐brain endocrine axis wherein Tα1 engages HCRTR1 to inhibit RIPK3‐mediated necroptosis. This study nominates the Tal‐HCRTR1‐RIPK3 axis as a potent and clinically translatable therapeutic target for stroke and neurodegeneration.
Xinmei Kang +11 more
wiley +1 more source
gRNA generated using ribozymes is as efficient as gRNA generated by transcription alone.
, 2016 PAGE analysis of smo 5’ UTR shows the formation of heteroduplex after injection of gRNA and Cas9 mRNA compared to the uninjected control. Comparison of intensity of heteroduplex and homoduplex shows that gRNA with or without ribozymes have similar ...
Ashley Shu Mei Ng (493205) +2 more
core +1 more source
Advanced Science, EarlyView.LRRK2‐mutant induced pluripotent stem cells (iPSCs) were derived from a patient with Parkinson's disease (PD). Using CRISPR/Cas9–mediated gene editing, the pathogenic LRRK2 mutations were precisely corrected, and isogenic dopaminergic neural progenitor cells (DA‐NPCs) were subsequently generated.
Qing Yan +29 more
wiley +1 more source