Results 61 to 70 of about 46,126 (304)
Viruses, 2022 Retroviruses must selectively recognize their unspliced RNA genome (gRNA) among abundant cellular and spliced viral RNAs to assemble into newly formed viral particles.
Serena Bernacchi
doaj +1 more source
gRNA sequences and characteristics.
, 2016 gRNA sequences and characteristics.
Jordan C. Crampton (2806165) +3 more
core +1 more source
Sheet 1: computational output showing gRNA properties.
, 2021 Sheet 2: gRNA oligonucleotide sequences ordered for cloning into pCFD3.1 vector. (XLSX)
Enas Gad El-Karim (11632158) +3 more
core +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Nghiên cứu tạo phôi chuột knockout gene lep sử dụng kỹ thuật CRISPR/Cas9
Tạp chí Khoa học Đại học Cần Thơ, 2022 Kỹ thuật CRISPR/Cas 9 đang được sử dụng nhiều trong công nghệ chỉnh sửa gene. Trong nghiên cứu này, phôi chuột knockout gene Lep được tạo ra bằng cách vi tiêm plasmid có cấu trúc CRISPR/Cas9 hướng đến exon 3 của gene.
Âu Dương Tuyết Mai +6 more
doaj +1 more source
Interactions of mRNAs and gRNAs involved in trypanosome mitochondrial RNA editing: Structure probing of a gRNA bound to its cognate mRNA [PDF]
RNA, 2006 Expression of mitochondrial genes in Trypanosoma brucei requires RNA editing of its mRNA transcripts. During editing, uridylates are precisely inserted and deleted as directed by the gRNA template to create the protein open reading frame. This process involves the bimolecular interaction of the gRNA with its cognate pre-edited mRNA and the assembly of ...
Laura E, Yu, Donna J, Koslowsky
openaire +2 more sources
Construction and evaluation of gRNA arrays for multiplex CRISPR‐Cas9
Yeast, 2023 AbstractEndonuclease system CRISPR‐Cas9 represents a powerful toolbox for the budding yeast's Saccharomyces cerevisiae genome perturbation. The resulting double‐strand breaks are preferentially repaired via highly efficient homologous recombination, which subsequently leads to marker‐free genome editing.
Gašper Žun +2 more
openaire +2 more sources
GM130 specific gRNA sequences.
, 2019 GM130 specific gRNA sequences.
Kati Tormanen (6589415) +4 more
core +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source