Results 41 to 50 of about 46,126 (304)

S100A8 gRNA sequences in CRISPR/Cas9 vector.

, 2021
S100A8 gRNA sequences in CRISPR/Cas9 vector.
Lei Li (29537), Hong Su (415707), Guifang Cao (3451934), Hongxin Yang (5705846), Moning Liu (3451931), Xiaodan Li (132227), Daqing Wang (406452), Dafu Zhi (3451922)   +7 more
core   +1 more source

Study of the effect of the introduction of mitochondrial import determinants into the gRNA structure on the activity of the gRNA/SpCas9 complex in vitro

Vavilov Journal of Genetics and Breeding, 2020
It has long been known that defects in the structure of the mitochondrial genome can cause various neuromuscular and neurodegenerative diseases. Nevertheless, at present there is no effective method for treating mitochondrial diseases. The major problem with the treatment of such diseases is associated with mitochondrial DNA (mtDNA) heteroplasmy.
E. G. Zakirova, Y. V. Vyatkin, N. A. Verechshagina, V. V. Muzyka, I. O. Mazunin, K. E. Orishchenko   +5 more
openaire   +4 more sources

RNA-Binding Domains of Heterologous Viral Proteins Substituted for Basic Residues in the RSV Gag NC Domain Restore Specific Packaging of Genomic RNA

Viruses, 2020
The Rous sarcoma virus Gag polyprotein transiently traffics through the nucleus, which is required for efficient incorporation of the viral genomic RNA (gRNA) into virus particles.
Breanna L. Rice, Timothy L. Lochmann, Leslie J. Parent   +2 more
doaj   +1 more source

Bar-cas12a, a novel and rapid method for plant species authentication in case of Phyllanthus amarus Schumach. & Thonn

Scientific Reports, 2021
Rapid and accurate species diagnosis accelerates performance in numerous biological fields and associated areas. However, morphology-based species taxonomy/identification might hinder study and lead to ambiguous results.
Kittisak Buddhachat, Suphaporn Paenkaew, Nattaporn Sripairoj, Yash Munnalal Gupta, Waranee Pradit, Siriwadee Chomdej   +5 more
doaj   +1 more source

Comparative Analysis of Cell Dilution Assays for Single-Cell Colony Formation in CRISPR-Mediated Knockout Screening

Cyprus Journal of Medical Sciences
BACKGROUND/AIMS: The ribonucleic acid (RNA)-guided Cas9 nuclease from the microbial clustered regularly interspaced palindromic repeats (CRISPR) immune system enables genome editing in eukaryotic cells by using a 20-nucleotide target sequence guide RNA ...
Hülya Dönmez, Bahadır Batar, Burhan Turgut   +2 more
doaj   +1 more source

Optimized Plasmid Construction Strategy for Cas9

Cellular Physiology and Biochemistry, 2018
Background/Aims: The target genome editing technology not only plays an important role in basic biology studies but also holds a great promise for potential clinical applications.
Jianyong  Xu, Wenlei Li, MD. Munnaf  Hossen, Yuning Jia, Lingyun Li, Zhong Huang   +5 more
doaj   +1 more source

gRNA-target-sites for CCSAS paper (Zhong et al., 2021 Microbiome)

, 2023
This dataset contains CRISPR-Cas9 compatible and taxon-specific gRNA-target-site sequences, specifically designed to target host 18S rRNA genes, without affecting microeukaryotes.
Kevin Zhong (16470363)
core   +1 more source

CRISPR‐TAPE: protein‐centric CRISPR guide design for targeted proteome engineering

Molecular Systems Biology, 2020
Rational molecular engineering of proteins with CRISPR‐based approaches is challenged by the gene‐centric nature of gRNA design tools. To address this, we have developed CRISPR‐TAPE, a protein‐centric gRNA design algorithm that allows users to target ...
Daniel Paolo Anderson, Henry James Benns, Edward William Tate, Matthew Andrew Child   +3 more
doaj   +1 more source

List of gRNA cassettes used in this study for simultaneous gene mutagenesis by CRISPR-Cas9.

, 2021
Sequences of gRNA cassette fragment (HH–20 bp specific determinant sequences–structural gRNA–HDV) with the addition of EcoRI and KpnI restriction sites for pOtAOX-gRNA plasmids construction in this study. EcoRI (gaattc) and KpnI (ggtacc) sequences are in
Sutipa Tanapongpipat (3126720), Aekkachai Puseenam (2902434), Niran Roongsawang (2902437), Worarat Kruasuwan (11495350)   +3 more
core   +1 more source

Cas9 gRNA engineering for genome editing, activation and repression [PDF]

Nature Methods, 2015
We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill ...
Samira Kiani, Alejandro Chavez, Marcelle Tuttle, Richard N Hall, Raj Chari, Dmitry Ter-Ovanesyan, Jason Qian, Benjamin W Pruitt, Jacob Beal, Suhani Vora, Joanna Buchthal, Emma J K Kowal, Mohammad R Ebrahimkhani, James J Collins, Ron Weiss, George Church   +15 more
openaire   +5 more sources