Results 41 to 50 of about 44,236 (298)

RNA-Binding Domains of Heterologous Viral Proteins Substituted for Basic Residues in the RSV Gag NC Domain Restore Specific Packaging of Genomic RNA

Viruses, 2020
The Rous sarcoma virus Gag polyprotein transiently traffics through the nucleus, which is required for efficient incorporation of the viral genomic RNA (gRNA) into virus particles.
Breanna L. Rice, Timothy L. Lochmann, Leslie J. Parent   +2 more
doaj   +1 more source

A mRNA determinant of gRNA-directed kinetoplastid editing [PDF]

Nucleic Acids Research, 2001
Several mitochondrial mRNAs of the kinetoplastid protozoa do not encode a functional open reading frame until they have been edited through the addition or deletion of U nucleotides at specific sites. Genetic information specifying the location and extent of editing is present on guide RNAs (gRNAs).
A L, Kabb, L M, Oppegard, B A, McKenzie, G J, Connell   +3 more
openaire   +2 more sources

The gRNA-miRNA-gRNA Ternary Cassette Combining CRISPR/Cas9 with RNAi Approach Strongly Inhibits Hepatitis B Virus Replication [PDF]

, 2017
The CRISPR/Cas9 system is a novel genome editing technology which has been successfully used to inhibit HBV replication. Here, we described a novel gRNA-microRNA (miRNA)-gRNA ternary cassette driven by a single U6 promoter.
Zhongwei Xu, Ningshao Xia, Hui Zhuang, Yuan, Quan, Chen, Xiangmei, Zhang, Ting, Zhang, Ruiyang, Frederick Nunes, Nunes, Frederick, Xu, Zhongwei, Zhang, Tianying, Ding, Shanlong, Shanlong Ding, Lu, Fengmin, Jie Wang, Quan Yuan, Block, Timothy, Zhongping Duan, Ran Chen, Guan, Guiwen, Shuang Liu, Xia, Ningshao, Timothy Block, Xiangmei Chen, Ruiyang Zhang, Wang, Jie, Chen, Ran, Zhuang, Hui, Duan, Zhongping, Tianying Zhang, Ting Zhang, Guiwen Guan, Fengmin Lu, Liu, Shuang   +33 more
core   +1 more source

Optimized Plasmid Construction Strategy for Cas9

Cellular Physiology and Biochemistry, 2018
Background/Aims: The target genome editing technology not only plays an important role in basic biology studies but also holds a great promise for potential clinical applications.
Jianyong  Xu, Wenlei Li, MD. Munnaf  Hossen, Yuning Jia, Lingyun Li, Zhong Huang   +5 more
doaj   +1 more source

CRISPR-Cas9 Dual-gRNA Attack Causes Mutation, Excision and Inversion of the HIV-1 Proviral DNA

Viruses, 2020
Although several studies demonstrated that the HIV proviral DNA can be effectively targeted and inactivated by the CRISPR-Cas9 system, the precise inactivation mechanism has not yet been analyzed.
Caroline S. Binda, Bep Klaver, Ben Berkhout, Atze T. Das   +3 more
doaj   +1 more source

Selected piezo1 gRNA creates indels in target sequence.

, 2023
(A) Sanger sequencing of 3 individual animals following injection with a scrambled gRNA along with Cas9 (Top). Sanger sequencing of 3 individual animals following injection with a piezo1 gRNA along with Cas9 (Bottom).
Cody J. Smith (6630881), Jacob P. Brandt (11725253)   +1 more
core   +1 more source

Comparative Analysis of Cell Dilution Assays for Single-Cell Colony Formation in CRISPR-Mediated Knockout Screening

Cyprus Journal of Medical Sciences
BACKGROUND/AIMS: The ribonucleic acid (RNA)-guided Cas9 nuclease from the microbial clustered regularly interspaced palindromic repeats (CRISPR) immune system enables genome editing in eukaryotic cells by using a 20-nucleotide target sequence guide RNA ...
Hülya Dönmez, Bahadır Batar, Burhan Turgut   +2 more
doaj   +1 more source

Cas9 gRNA engineering for genome editing, activation and repression [PDF]

Nature Methods, 2015
We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill ...
Samira Kiani, Alejandro Chavez, Marcelle Tuttle, Richard N Hall, Raj Chari, Dmitry Ter-Ovanesyan, Jason Qian, Benjamin W Pruitt, Jacob Beal, Suhani Vora, Joanna Buchthal, Emma J K Kowal, Mohammad R Ebrahimkhani, James J Collins, Ron Weiss, George Church   +15 more
openaire   +5 more sources

CRISPR‐TAPE: protein‐centric CRISPR guide design for targeted proteome engineering

Molecular Systems Biology, 2020
Rational molecular engineering of proteins with CRISPR‐based approaches is challenged by the gene‐centric nature of gRNA design tools. To address this, we have developed CRISPR‐TAPE, a protein‐centric gRNA design algorithm that allows users to target ...
Daniel Paolo Anderson, Henry James Benns, Edward William Tate, Matthew Andrew Child   +3 more
doaj   +1 more source

On the Selective Packaging of Genomic RNA by HIV-1

Viruses, 2016
Like other retroviruses, human immunodeficiency virus type 1 (HIV-1) selectively packages genomic RNA (gRNA) during virus assembly. However, in the absence of the gRNA, cellular messenger RNAs (mRNAs) are packaged.
Mauricio Comas-Garcia, Sean R. Davis, Alan Rein   +2 more
doaj   +1 more source