Results 111 to 120 of about 228,880 (307)

MOESM1 of CRISPR-Cas9-mediated knockout of SPRY2 in human hepatocytes leads to increased glucose uptake and lipid droplet accumulation

, 2019
Additional file 1: Supplementary Methods. Figure S1. Overview of the SPRY2 locus. Figure S2. Exon organisation of SPRY2 gene. Figure S3. Examples of DNA mutations generated following CRISPR-Cas9 genome editing for SPRY2 in HepG2 cells. Figure S4.
Abhiram Rao (7587503), Casimiro Castillejo-Lรณpez (7587512), Erik Ingelsson (232651), Susanne Hetty (7587506), Milos Pjanic (485288), Naomi Cook (4738857), Thomas Quertermous (94604), Joshua Knowles (116765), Andrew Emmerich (7587500)   +8 more
core   +1 more source

Ionic Gating of Liquid‐Crystal‐Like Pteridine Assemblies Enables Tunable Light Scattering

Advanced Materials, EarlyView.
Tunable optical properties emerge through developmentally regulated ionic gating, which transforms disordered, ultraviolet (UV)‐absorbing organelles into concentrically ordered, liquid‐crystal‐like light scatterers. By integrating analyses of pteridine composition, potassium‐dependent assembly, hierarchical ultrastructure, and optical behavior, the ...
Sourabh Bera, Zohar Eyal, Yael Noy, Yuval Barzilay, Siddharth Sahoo, Srikant Moharana, Avi Baram, Tali Lerer‐Goldshtein, Neta Varsano, Bar Bader, Lothar Houben, Uwe Heinig, Iddo Pinkas, Moshe Goldsmith, Tamar Unger, Shira Albeck, Andrea Sorrentino, Maxim Itkin, Sergey Malitsky, Ziv Porat, Ido Hadar, Venkata Jayasurya Yallapragada, Dvir Gur   +22 more
wiley   +1 more source

Spacer sequence for targeting gyrA gene in Bacillus subtilis strain 168 using CRISPR-Cas9

, 2019
Guide RNA provides unique targeting capability to CRISPR-Cas9 genome editing system through complementary base pairing with the DNA strand at specific locus.
Wenfa Ng (444092)
core   +1 more source

Microfluidic Droplet Plate Preserving Single Human Induced Pluripotent Stem Cell‐Derived Cardiomyocytes Morphology and Function

Advanced Materials Technologies, EarlyView.
We introduce a novel method combining microfluidics, optogenetics, and human induced pluripotent stem cell (iPSC) technology to encapsulate single iPSC‐derived cardiomyocytes (iPSC‐CMs) in an optimal environment for functional characterization. This method enables direct genotype‐phenotype correlation for genetic studies and high‐throughput screening ...
Xiao‐Ting Wang, Rachel P. White, Yashasvi Tharani, Michael A. Finocchiaro, Leona M. Ripple, Maksymilian Prondzynski, Tania Konry, Vassilios J. Bezzerides   +7 more
wiley   +1 more source

UGI relocation inside Cas9 reduces Cas9 dependent off target effects in cytosine base editors

Scientific Reports
Cytosine base editors (CBEs) achieve precise C-to-T conversions by addition of uracil DNA glycosylase inhibitor (UGI) with Cas9 nickase (nCas9) and cytidine deaminase, and the conventional fusion at the nCas9 carboxyl terminus effectively inhibits uracil
Zehao Shi, Tian-Lin Cheng
doaj   +1 more source

Table_1_Quality Control Strategy for CRISPR-Cas9-Based Gene Editing Complicated by a Pseudogene.docx

, 2020
CRISPR-Cas9 mediated gene editing in induced pluripotent stem cells became an efficient tool to investigate biological mechanisms underlying genetic-driven diseases while accounting for the respective genetic background.
Zoé Hanss (8267268), Rejko Krüger (252762), Ibrahim Boussaad (8267271), Jens C. Schwamborn (8267274), Javier Jarazo (6453035)   +4 more
core   +1 more source

Intelligent Micro/Nanorobots for Targeted Interventional Therapy: From Bench to Clinic

Advanced Robotics Research, EarlyView.
Zirui Zhang et al. reviewed the application and challenges of mobile nanomachines in interventional therapy. By converting exogenous energy, including chemical, magnetic, optical, and ultrasonic sources, into mechanical forces, micro/nanorobots (MNRs) enable precise actuation at unprecedented scales. Evolving far beyond traditional drug delivery, these
Zirui Zhang, Yu Dong, Yongsheng Du, Rui Gao, Lin Zhang, Fei Tong   +5 more
wiley   +1 more source

Coixenolide Enhances Antitumor Immunity of Cytotoxic CD8+ T Cells by Inhibiting S100A8/A9‐CD36 Axis in Obesity‐Associated Pancreatic Adenocarcinoma

Advanced Science, EarlyView.
Pancreatic adenocarcinoma (PAAD) remains highly lethal with limited treatment options. This study demonstrates that coixenolide, a bioactive compound from Coix lacryma‐jobi L. and a key component of the clinically approved Kanglaite injection, exhibits enhanced antitumor efficacy in high‐fat diet (HFD)‐induced obese mice bearing PAAD tumors compared to
Kaidi Chen, Rong Chen, Wei Li, Bin Song, Ruiying Zhang, Lewei Dai, Yuwei Qi, Ruqian Chen, Yifan Xu, Hangbin Li, Jingyi Huang, Fangping Wu, Jianan Peng, Xinyu Wang, Yang Xiong, Chaofeng Mu, Guilin Cheng, Zhaoshen Li, Shanming Ruan, Lei Huang, Mancang Gu   +20 more
wiley   +1 more source

Development of an RNA aptamer-assisted CRISPR/Cas9 system for efficiently generating and isolating Cas9-free mutants in plant.

PLoS Genetics
The CRISPR/Cas9 gene-editing system is a powerful tool in plant genetic engineering; however, screening for Cas9-free edited plants remains complex and time-consuming.
Sha Liu, Jiuyuan Bai, Bo Zhan, Junyu Yao, Jiayu Zhang, Jia Yi, Mengyue Dong, Qicong Li, Yucheng Shen, Yazhou Chen, Yun Zhao   +10 more
doaj   +1 more source

DataSheet_1_Quality Control Strategy for CRISPR-Cas9-Based Gene Editing Complicated by a Pseudogene.pdf

, 2020
CRISPR-Cas9 mediated gene editing in induced pluripotent stem cells became an efficient tool to investigate biological mechanisms underlying genetic-driven diseases while accounting for the respective genetic background.
Zoé Hanss (8267268), Rejko Krüger (252762), Ibrahim Boussaad (8267271), Jens C. Schwamborn (8267274), Javier Jarazo (6453035)   +4 more
core   +1 more source