Results 181 to 190 of about 251,083 (336)

Nano-Polymers as Cas9 Inhibitors

Polymers
Despite wide applications of CRISPR/Cas9 technology, effective approaches for CRISPR delivery with functional control are limited. In an attempt to develop a nanoscale CRSIPR/Cas9 delivery platform, we discovered that several biocompatible polymers, including polymalic acid (PMLA), polyglutamic acid (PGA), and polyaspartic acid (PLD), when conjugated ...
Oksana Chepurna, Avradip Chatterjee, Yuanqing Li, Hong Ding, Ramachandran Murali, Keith L. Black, Tao Sun   +6 more
openaire   +2 more sources

From Code to Life: The AI‐Driven Revolution in Genome Editing

Advanced Science, EarlyView.
This review explores AI‐driven advancements in genome editing, focusing on CRISPR optimization, enhanced targeting precision, reduced off‐target effects, and novel tool design. It addresses ethical challenges, data biases, and highlights future prospects in precision medicine, agriculture, and synthetic biology.
Zhidong Li, Wasi Ullah Khan, Genxiang Bai, Chao Dong, Jungang Wang, Youpeng Zhang, Chong Wang, Hongbin Zhang, Wenyi Wang, Ming Luo, Fei Chen   +10 more
wiley   +1 more source

Enhanced Specificity and Efficiency of the CRISPR/Cas9 System with Optimized sgRNA Parameters in Drosophila [PDF]

, 2014
Xingjie Ren, Zhihao Yang, Xu Jiang, Jin Sun, Decai Mao, Yanhui Hu, Sujuan Yang, Huanhuan Qiao, Xia Wang, Qun Hu, Patricia Deng, Luping Liu, Jun‐Yuan Ji, Jin Billy Li, Jian‐Quan Ni   +14 more
openalex   +1 more source

Metabolic Stress‐Induced Choline Kinase α (CHKA) Activation in Endothelial Subpopulation Contributes to Diabetes‐Associated Microvascular Dysfunction

Advanced Science, EarlyView.
This study uncovers CHKA as a pivotal driver of vascular dysfunction in diabetic retinopathy and highlights its role in endothelial dysfunction through NAD⁺‐SIRT1‐Notch signaling. Single‐cell and functional analyses, combined with clinical and genetic validation, suggest CHKA as a promising therapeutic target for the prevention and treatment of ...
Ling Ren, Linyu Zhang, Yun Bai, Chang Huang, Xiaosa Li, Fanfei Ma, Wan Mu, Mudi Yao, Chang Jiang, Xiangjun Chen, Qin Jiang, Biao Yan   +11 more
wiley   +1 more source

Faculty Opinions recommendation of DNA targeting specificity of RNA-guided Cas9 nucleases.

, 2013
Blake Wiedenheft
openalex   +2 more sources

Targeting non-coding RNAs with the CRISPR/Cas9 system in human cell lines [PDF]

, 2014
Tsui-Ting Ho, Nanjiang Zhou, Jianguo Huang, Pratirodh Koirala, Min Xu, Roland Fung, Fangting Wu, Yin‐Yuan Mo   +7 more
openalex   +1 more source

Cas9-directed immunity [PDF]

Nature Immunology, 2018
openaire   +2 more sources

Small RNA Toxin‐Assisted Evolution of GC‐Preferred ErCas12a for Enhanced Genome Targeting Range

Advanced Science, EarlyView.
ErCas12a is engineered to target GC‐rich PAMs using a small RNA toxin‐aided positive screening system. The resulting variant, enErCas12a, exhibits an expanded PAM profile and facilitates efficient gene editing in both bacterial and mammalian cells, while preserving high targeting specificity for both canonical and non‐canonical PAM targets.
Zehua Chen, Junyuan Xue, Ziying Wang, Jinyuan Sun, Yinglu Cui, Tong Zhu, Huaiyi Yang, Ming Li, Bian Wu   +8 more
wiley   +1 more source

Instantaneous visual genotyping and facile site-specific transgenesis via CRISPR-Cas9 and phiC31 integrase

Biology Open
Junyan Ma, Weiting Zhang, Simin Rahimialiabadi, Nikkitha Umesh Ganesh, Zhengwang Sun, Saba Parvez, Randall T. Peterson, Jing-Ruey Joanna Yeh   +7 more
doaj   +1 more source

Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination

, 2013
Daniel J. Dickinson, Jordan D. Ward, David J. Reiner, Bob Goldstein   +3 more
openalex   +2 more sources