Results 171 to 180 of about 228,880 (307)

OAF Blocks SIAH1‐Mediated Degradation of SCPX, a Therapeutic Strategy for MASLD

Advanced Science, EarlyView.
This study shows OAF directly binds to SCPX and inhibits its interaction with the E3 ligase SIAH1, thereby protecting SCPX from ubiquitin‐dependent degradation and stabilizing its levels. This OAF‐SCPX axis represents a novel pathway in lipid homeostasis, highlighting OAF as a promising therapeutic candidate for MASLD.
Zongxi Li, Jingyue Song, Kaili Ma, Shunan Tang, Shuqian Wang, Wenya Ji, Chen Lu, Ting Zhang, Huaping Zheng, Xiaowei Zheng, Shanshan Lai, Cheng Deng   +11 more
wiley   +1 more source

Generación de una colección de líneas celulares Cas9+ para estudios de edición genómica mediante CRISPR/Cas9

, 2019
[spa] La edición genómica basada en el sistema CRISPR/Cas9 se ha convertido en una herramienta central para estudios de la función génica en modelos celulares. Consecuentemente, resulta también de gran interés utilizar esta metodología para el estudio de
García Fernández, Miguel
core  

Grtp1 Safeguards Against Paternal Reproductive Aging and Intergenerational Behavioral Deficits by Preserving Redox Homeostasis

Advanced Science, EarlyView.
This study identifies that Grtp1 maintains germline redox homeostasis by interacting with PRDX1/4‐TXN to antagonize paternal reproductive aging. Growth hormone restores Grtp1 expression, rectifies aberrant sperm DNA methylation, and ameliorates intergenerational anxiety and social deficits, highlighting the GH‐GRTP1 axis as a promising intervention ...
Yingdong Liu, Shanyao Liu, Haixin Liang, Jiani Yang, Yanhe Li, Jiqing Yin, Xueguang Zhang, Yan Zhang, Dandan Bai, Zihui Yan, Zhuoao Zhang, Yanping Jia, Kuisheng Liu, Yifan Sheng, Jiani Xiang, Chenxiang Xi, Baoxing Dong, Xinyi Lei, Jiayu Chen, Hong Wang, Yi Guo, Yanping Zhang, Shaorong Gao, Wenqiang Liu   +23 more
wiley   +1 more source

Insight on CRISPR-Cas9 genome editing technology: optimization and application in a nematode model.

reservedIl sistema di editing genomico CRISPR-Cas9 ha rivoluzionato il campo della manipolazione genetica. Questa tesi si concentra sull'ottimizzazione e l'applicazione di CRISPR-Cas9 in Caenorhabditis elegans al fine di riprodurre una specifica variante
HUDA, SUADA
core  

Optimized Cas9‐Enriched Nanopore Sequencing and Analysis Workflow for Clinical Diagnosis of Repeat Expansion Disorders

Advanced Science, EarlyView.
An optimized Cas9‐enriched nanopore sequencing workflow, combined with STRiker, enables simultaneous analysis of disease‐associated STR loci from patient blood. The nCATS–STRiker workflow detects repeat expansions, de novo repeat motifs, interruption patterns, and methylation in a single assay, improving the genetic diagnosis of previously undiagnosed ...
Seungbok Lee, Chanju Jung, Minjeong Kim, Narae Kim, Gue‐Ho Hwang, Soon‐Tae Lee, Kon Chu, Sang Kun Lee, Han‐Joon Kim, Jong‐Hee Chae, Sangsu Bae, Jangsup Moon   +11 more
wiley   +1 more source

In Vivo CRISPR Screen Identifies RPS19 as an Immunotherapy Target in Hepatocellular Carcinoma. [PDF]

Adv Sci (Weinh)
He L, Yu D, Xie F, Hou K, Li Z, Dai M, Yun J, Cao H, Xiao Y, Qiu Y, Shi G, Zhang W, Wang S, Hao Q, Zhang J, Wang Z, Zhang Y, Zhang K, Gu J, Du H, Li M.   +20 more
europepmc   +1 more source

Glucose and Glutamine Deprivation Promotes Breast Cancer Lung Metastasis via BRD4‐Dependent Enhancer Activation

Advanced Science, EarlyView.
To model a nutrient‐restricted tumor microenvironment, breast cancer cells were adapted to low glucose and glutamine levels. In the adapted cells, BRD4‐driven enhancer activation increases pro‐invasive EDN1 expression, facilitated by ATF3/c‐JUN and reinforced by enhancer RNA.
Poshan Yugal Bhattarai, Arathy Vasukutty, Yujin Na, Sung‐Chul Lim, Hong Seok Choi   +4 more
wiley   +1 more source

CRISPR-Cas9 in Cancer Therapy: Overcoming Delivery Barriers and Off-Target Effects for Clinical Translation. [PDF]

Health Sci Rep
Mohiuddin M, Ahsan MAM.
europepmc   +1 more source

Deubiquitination of Vangl by USP6 and USP32 Regulates Planar Cell Polarity Signaling

Advanced Science, EarlyView.
Compartment‐specific deubiquitination controls Vangl dosage and planar cell polarity signaling. USP6 and USP32 regulate distinct subcellular pools of Vangl by removing distinct ubiquitin modifications from Vangl proteins. This regulatory mechanism safeguards PCP‐dependent embryonic morphogenesis, while aberrant USP32‐dependent stabilization of VANGL ...
Fangzi Zha, Di Feng, Ziru Xue, Zhiying Liu, Xiaochen Lin, Zhe Wang, Ling Xu, Jasper Fuk Woo Chan, Martin Cheung, Michael Shing Yan Huen, Yusong Guo, Kui Ming Chan, Yun Liu, Bo Gao   +13 more
wiley   +1 more source

In situ CAR-M engineered by liposomal mRNA nanomedicine remodels fibrotic microenvironment for ameliorating liver fibrosis. [PDF]

Sci Adv
Xiao H, Li X, Cai K, Leng Y, Miao X, Yin T, Shuai X, Ren J.   +7 more
europepmc   +1 more source