Results 151 to 160 of about 200,296 (309)
Insight on CRISPR-Cas9 genome editing technology: optimization and application in a nematode model.
reservedIl sistema di editing genomico CRISPR-Cas9 ha rivoluzionato il campo della manipolazione genetica. Questa tesi si concentra sull'ottimizzazione e l'applicazione di CRISPR-Cas9 in Caenorhabditis elegans al fine di riprodurre una specifica variante
HUDA, SUADA
core
Advanced Science, EarlyView.The hyperactivation of PI3K/AKT signaling in PTEN wild‐type triple‐negative breast cancer represents a clinical paradox. We delineate a novel post‐translational regulatory axis wherein the oncogene TSPYL5 competitively antagonizes the deubiquitinase USP10.
Jiaying Shi +8 more
wiley +1 more source
Structural insights into Cas9 mismatch: promising for development of high-fidelity Cas9 variants
Signal Transduction and Targeted Therapy, 2022 Honghai Tang, Daqi Wang, Yilai Shu
doaj +1 more source
PSMA8‐Containing 20S Proteasome Regulates Spermiogenesis and Male Fertility
Advanced Science, EarlyView.PSMA8 assembles s20S proteasome that degrades specific substrates in elongating spermatids. Degradation of s20S‐substrates activates translation of FXR1‐target mRNAs, which are essential for mitochondrial sheath formation and sperm morphogenesis.
Huiwen Cao +7 more
wiley +1 more source
Использование технологии CRISPR/Cas9 для получения мутантов по генам транспортеров серотонина у Danio rerio [PDF]
, 2018 Технология CRISPR/Cas9 – новейший метод редактирования геномов, основанный на адаптивном иммунитете прокариот. Для её применения необходим особый РНК-гид, направляющий эффекторную нуклеазу этой системы к специфической мишени в геноме, и сама нуклеаза ...
Кисель Элана Вадимовна +1 more
core
Ultrasound‐Actuated Gene Editing in Human Kidney Organoids
Advanced Science, EarlyView.Peptide‐stabilized nanoemulsions enable spatially controlled, ultrasound‐triggered delivery of gene‐editing proteins deep within human kidney organoid tissues. Focused acoustic pulses drive droplet vaporization, propelling cargo into target renal cells while preserving native tissue microarchitecture. This non‐viral platform improves both the depth and
Michael A. Miller +6 more
wiley +1 more source
Standardized Iterative Genome Editing Method for Escherichia coli Based on CRISPR-Cas9
The introduction of complex biosynthetic pathways into the hosts’ chromosomes is gaining attention with the development of synthetic biology. While CRISPR-Cas9 has been widely employed for gene knock-in, the process of multigene insertion remains ...
Jianghua Zhao (1745965) +5 more
core +1 more source
Advanced Science, EarlyView.This study constructed the first spatiotemporal multi‐omics map of peach fruit and discovered a key candidate gene that synergistically regulates trichome development and drought tolerance through the jasmonic acid signaling pathway, providing insights into the coupling mechanism between development and stress resistance.
Zhixin Liu +9 more
wiley +1 more source
Redox‐Dependent Chaperoning of GBF1 Condensates Regulates Seed Germination in Arabidopsis
Advanced Science, EarlyView.In dormant seeds (low ROS), GBF1 forms liquid condensates to repress the germination gene CathB3, and the chaperone GIP1 maintains condensate liquidity and repressive activity. Upon imbibition (high ROS), ROS oxidize GIP1 during germination, impairing its chaperone function.
Yunying Wang, Xiaofeng Fang
wiley +1 more source