Results 221 to 230 of about 251,083 (336)

De Novo Variant in GBX1 Gene Associated With Developmental Delay and Focal Epilepsy. [PDF]

Mol Genet Genomic Med
Zhang B, Li X, Qian X, Tang J.
europepmc   +1 more source

Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9

, 2014
Pankaj Kumar Mandal, Leonardo M. R. Ferreira, Ryan L. Collins, Torsten Meißner, Christian L. Boutwell, Max Friesen, Vladimir Vrbanac, Brian S. Garrison, Alexei Stortchevoi, David Bryder, Kiran Musunuru, Harrison Brand, Andrew M. Tager, Todd M. Allen, Michael E. Talkowski, Derrick J. Rossi, Chad A. Cowan   +16 more
openalex   +1 more source

495. Adenoviral Vector Delivery of RNA-Guided CRISPR/Cas9 Nuclease Complexes Induces Targeted Mutagenesis in a Diverse Array of Human Cells [PDF]

, 2014

openalex   +1 more source

Mechanical Load‐Induced Upregulation of Talin2 through Non‐Canonical Deubiquitination of OTUB1 Drives Facet Joint Osteoarthritis Pathogenesis

Advanced Science, EarlyView.
Facet joint osteoarthritis (FJOA), prevalent in the elderly, involves Talin2 upregulation promoting ECM degradation while suppressing synthesis. Mechanical load stabilizes Talin2 via OTUB1‐mediated non‐canonical deubiquitination, driving CCL2 expression that recruits immune cells, exacerbating inflammatory joint degeneration.
Yizhen Huang, Heng Sun, Haojie Chen, Xiangpeng Wang, Junduo Zhao, Yang Jiao, Hongyi Zhou, Haoyu Cai, Jiafeng Dai, Xuan Huang, Weiyun Chen, Jianxiong Shen   +11 more
wiley   +1 more source

A rational engineering strategy for structural dynamics modulation enables target specificity enhancement of the Cas9 nuclease. [PDF]

Nucleic Acids Res
Sung K, Jung Y, Kim N, Kim YW, Kim HH, Kim SK, Bae S.   +6 more
europepmc   +1 more source

Whole-Genome Sequencing Analysis Reveals High Specificity of CRISPR/Cas9 and TALEN-Based Genome Editing in Human iPSCs

, 2014
Cory Smith, Athurva Gore, Wei Yan, Leire Abalde-Atristain, Zhe Li, Chaoxia He, Ying Wáng, Robert A. Brodsky, Kun Zhang, Linzhao Cheng, Zhaohui Ye   +10 more
openalex   +1 more source

Gut Metabolite Indole‐3‐Propionic Acid Regulates Macrophage Autophagy Through PPT1 Inhibiting Aging‐Related Myocardial Fibrosis

Advanced Science, EarlyView.
IPA is an intestinal tryptophan metabolite whose effects decline with decreased heart function. Supplementing IPA can alleviate the aging‐related myocardial fibrosis through PPT1. PPT1 is a key protein localized to lysosomes, and IPA can restore macrophage autophagy function by regulating PPT1 expresssions, thereby reducing aging‐related myocardial ...
Jing Lu, Hongyan Wang, Haiyu Zhang, Jiahao Li, Hanqi Li, Qiuyu Chen, Dongyu Han, Jialiang Liu, Lin Lv, Jie Xiong, Keying Yuan, Xianpeng Wei, Siqi Sheng, Fukai Liu, Yuanqi Shi, Zengxiang Dong, Yue Li   +16 more
wiley   +1 more source

A CRISPR-Cas9 system for knock-out and knock-in of high molecular weight DNA enables module-swapping of the pikromycin synthase in its native host. [PDF]

Microb Cell Fact
Wang ZC, Stegall H, Miyazawa T, Keatinge-Clay AT.   +3 more
europepmc   +1 more source

Facilitating crRNA Design by Integrating DNA Interaction Features of CRISPR‐Cas12a System

Advanced Science, EarlyView.
This study introduces a novel approach combining molecular dynamics simulations and neural network modeling to predict the Cas12a trans‐cleavage activity. By integrating sequence and molecular interaction features, prediction accuracy is enhanced, and identify key features affecting Cas12a trans‐cleavage activity.
Zhihao Yao, Wanglu Li, Kaiyu He, Hongmei Wang, Yan Xu, Qun Wu, Liu Wang, Yao Nie   +7 more
wiley   +1 more source

Transdermal delivery of CRISPR/Cas9-mediated melanoma gene therapy via polyamines-modified thermosensitive hydrogels. [PDF]

J Nanobiotechnology
Li M, Zhou S, Zhang S, Xie X, Nie J, Wang Q, Ma L, Cheng Y, Luo J.   +8 more
europepmc   +1 more source