Results 131 to 140 of about 4,561,775 (254)

Expression and Enzymatic Properties of Domain-Deleted Mutants of Cyclodextrin Glucosyltransferase my20 [PDF]

Shipin Kexue
In this study, we constructed domain D, E and DE truncated mutants of a marine-derived cyclodextrin glucosyltransferase (CGTase) my20 and heterologously expressed these mutants using the pET-24a vector and E. coli BL21 (DE3) as the host, and purified the
SUN Tengteng, WANG Wei, SUN Jingjing, JIANG Chengcheng, HAO Jianhua
doaj   +1 more source

Exploring alternative catalytic mechanisms of the Cas9 HNH domain

Proteins: Structure, Function, and Bioinformatics, 2019
AbstractUnderstanding the reaction mechanism of CRISPR‐associated protein 9 (Cas9) is crucial for the application of programmable gene editing. Despite the availability of the structures of Cas9 in apo‐ and substrate‐bound forms, the catalytically active structure is still unclear. Our first attempt to explore the catalytic mechanism of Cas9 HNH domain
Li Na Zhao, Dibyendu Mondal, Arieh Warshel   +2 more
openaire   +3 more sources

A switch element in the autophagy E2 Atg3 mediates allosteric regulation across the lipidation cascade

, 2019
Autophagy depends on the E2 enzyme, Atg3, functioning in a conserved E1-E2-E3 trienzyme cascade that catalyzes lipidation of Atg8-family ubiquitin-like proteins (UBLs).
Grace, C., Klionsky, D., Liu, X., Qiu, Y., Schulman, B., Zheng, Y.   +5 more
core   +1 more source

Mechanism of Diphtheria Toxin Catalytic Domain Delivery to the Eukaryotic Cell Cytosol and the Cellular Factors that Directly Participate in the Process

Toxins, 2011
Research on diphtheria and anthrax toxins over the past three decades has culminated in a detailed understanding of their structure function relationships (e.g., catalytic (C), transmembrane (T), and receptor binding (R) domains), as well as the ...
J. Murphy
semanticscholar   +1 more source

Crystal structure of an EAL domain in complex with reaction product 5'-pGpG.

PLoS ONE, 2012
FimX is a large multidomain protein containing an EAL domain and involved in twitching motility in Pseudomonas aeruginosa. We present here two crystallographic structures of the EAL domain of FimX (residues 438-686): one of the apo form and the other of ...
Julien Robert-Paganin, Sylvie Nonin-Lecomte, Stéphane Réty   +2 more
doaj   +1 more source

Role of Net Charge on Catalytic Domain and Influence of Cell Wall Binding Domain on Bactericidal Activity, Specificity, and Host Range of Phage Lysins*

Journal of Biological Chemistry, 2011
Background: Lytic enzymes (lysins) from bacteriophages are potential bactericidal agents. Results: Positive net charge on lysin catalytic domains is a major factor in determining lytic activity and host range.
L. Y. Low, Chen Yang, M. Perego, A. Osterman, R. Liddington   +4 more
semanticscholar   +1 more source

Multi-domain O-GlcNAcase structures reveal allosteric regulatory mechanisms

Nature Communications
Nucleocytoplasmic protein O-GlcNAcylation is a dynamic modification catalysed by O-GlcNAc transferase (OGT) and reversed by O-GlcNAc hydrolase (OGA), whose activities are regulated through largely unknown O-GlcNAc-dependent feedback mechanisms.
Sara Basse Hansen, Sergio G. Bartual, Huijie Yuan, Olawale G. Raimi, Andrii Gorelik, Andrew T. Ferenbach, Kristian Lytje, Jan Skov Pedersen, Taner Drace, Thomas Boesen, Daan M. F. van Aalten   +10 more
doaj   +1 more source

Molecular Modelling of PDE3A Catalytic Domain

, 2020
Recent studies have highlighted the clinical benefits of regulating phosphodiesterase-3 enzymes. Inhibition of PDE3A has proven to aid in preventing and treating cardiovascularrelated disorders and platelet dysfunction. Hypothermia is a condition that can cause cardiac arrest.
openaire   +1 more source

Allosteric activation of the nitric oxide receptor soluble guanylate cyclase mapped by cryo-electron microscopy. [PDF]

, 2019
Soluble guanylate cyclase (sGC) is the primary receptor for nitric oxide (NO) in mammalian nitric oxide signaling. We determined structures of full-length Manduca sexta sGC in both inactive and active states using cryo-electron microscopy. NO and the sGC-
Hammel, Michal, Horst, Benjamin G, Hurley, James H, Marletta, Michael A, Morris, Kyle L, Rosenberg, Daniel J, Yokom, Adam L   +6 more
core  

Understanding ATP Binding to DosS Catalytic Domain with a Short ATP-Lid. [PDF]

Biochemistry, 2023
Larson GW, Windsor PK, Smithwick E, Shi K, Aihara H, Rama Damodaran A, Bhagi-Damodaran A.   +6 more
europepmc   +1 more source