Results 131 to 140 of about 365,538 (313)

In vitro and in silico modelling of ROS1‐positive non‐small cell lung cancer reveals fusion‐dependent tyrosine kinase inhibitor responses

Molecular Oncology, EarlyView.
Drug resistance limits treatment success in a subset of lung cancers driven by ROS1 gene alterations. Using patient‐derived cells and computer simulations, we studied three key mutations and how they affect five targeted drugs. The mutations reduced drug effectiveness in different ways by altering protein structure and behavior.
Farhan Ul Haq, Marc Terrones, Felicia Rodrigues Fortes, Anne Schepers, Alessia Denis, Christophe Deben, Ken Op de Beeck, Guy Van Camp, Geert Vandeweyer   +8 more
wiley   +1 more source

Anti-staphylococcal activities of lysostaphin and LytM catalytic domain

BMC Microbiology, 2012
Background Lysostaphin and the catalytic domain of LytM cleave pentaglycine crossbridges of Staphylococcus aureus peptidoglycan. The bacteriocin lysostaphin is secreted by Staphylococcus simulans biovar staphylolyticus and directed against the cell walls
Sabala Izabela, Jonsson Ing-Marie, Tarkowski Andrej, Bochtler Matthias   +3 more
doaj   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Mechanistic insights into a cold-adapted glucokinase with high thermal stability were revealed by site-directed spin labeling ESR

Biophysics and Physicobiology
The structural flexibility of enzymes plays an essential role in determining their catalytic efficiency and thermal stability. Cold-adapted enzymes are typically highly flexible, resulting in high catalytic activity but low stability.
Akane Yato, Masaki Horitani
doaj   +1 more source

Crystal structure of an EAL domain in complex with reaction product 5'-pGpG.

PLoS ONE, 2012
FimX is a large multidomain protein containing an EAL domain and involved in twitching motility in Pseudomonas aeruginosa. We present here two crystallographic structures of the EAL domain of FimX (residues 438-686): one of the apo form and the other of ...
Julien Robert-Paganin, Sylvie Nonin-Lecomte, Stéphane Réty   +2 more
doaj   +1 more source

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source

Multi-domain O-GlcNAcase structures reveal allosteric regulatory mechanisms

Nature Communications
Nucleocytoplasmic protein O-GlcNAcylation is a dynamic modification catalysed by O-GlcNAc transferase (OGT) and reversed by O-GlcNAc hydrolase (OGA), whose activities are regulated through largely unknown O-GlcNAc-dependent feedback mechanisms.
Sara Basse Hansen, Sergio G. Bartual, Huijie Yuan, Olawale G. Raimi, Andrii Gorelik, Andrew T. Ferenbach, Kristian Lytje, Jan Skov Pedersen, Taner Drace, Thomas Boesen, Daan M. F. van Aalten   +10 more
doaj   +1 more source

The impact of the C-terminal domain on the interaction of human DNA topoisomerase II α and β with DNA [PDF]

, 2011
<b>Background</b> Type II DNA topoisomerases are essential, ubiquitous enzymes that act to relieve topological problems arising in DNA from normal cellular activity.
Caroline A. Austin, Kathryn L Gilroy, Caroline A Austin, Austin Caroline A., Austin, C.A., Gilroy Kathryn L., Gilroy, K.L., Kathryn L. Gilroy   +7 more
core   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

A prominent β-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation.

, 2010
RecQ helicases have attracted considerable interest in recent years due to their role in the suppression of genome instability and human diseases. These atypical helicases exert their function by resolving a number of highly specific DNA structures.
M. Berti, C. D. O. Cooper, Y. Zhang, Gileadi, Opher, Niesen, Frank H., O. King, Berti, M, Gileadi, O, A. C. W. Pike, Berti, Matteo, Vindigni, Alessandro, N. A. Burgess-Brown, Pike, AC, F. H. Niesen, King, O, Niesen, FH, A. Vindigni, Cooper, CD, R. Mendoza-Maldonado, King, Oliver, Burgess-Brown, Nicola, Mendoza-Maldonado, R, Vindigni, A, Zhang, Y, B. Lucic, Lucic, B, O. Gileadi, Burgess-Brown, NA, Pike, Ashley C.W., Lucic, Bojana, Zhang, Ying, Burgess-Brown, Nicola A., Mendoza-Maldonado, Ramiro, Cooper, Christopher D.O.   +33 more
core   +1 more source