Results 1 to 10 of about 2,074,425 (358)
Mechanisms of catalytic RNA molecules. [PDF]
Biochem Soc Trans, 2021 Ribozymes are folded catalytic RNA molecules that perform important biological functions. Since the discovery of the first RNA with catalytic activity in 1982, a large number of ribozymes have been reported. While most catalytic RNA molecules act alone, some RNA-based catalysts, such as RNase P, the ribosome, and the spliceosome, need protein ...
Alonso D, Mondragón A.
europepmc +4 more sources
RNA-catalyzed evolution of catalytic RNA. [PDF]
Proc Natl Acad Sci U S AAn RNA polymerase ribozyme that was obtained by directed evolution can propagate a functional RNA through repeated rounds of replication and selection, thereby enabling Darwinian evolution. Earlier versions of the polymerase did not have sufficient copying fidelity to propagate functional information, but a new variant with improved fidelity can ...
Papastavrou N, Horning DP, Joyce GF.
europepmc +4 more sources
Cryo-EM reveals an entangled kinetic trap in the folding of a catalytic RNA. [PDF]
Sci Adv, 2022 Functional RNAs fold through complex pathways that can contain misfolded “kinetic traps.” A complete model of RNA folding requires understanding the formation of these misfolded states, but they are difficult to characterize because of their transient ...
Bonilla SL, Vicens Q, Kieft JS.
europepmc +2 more sources
Recent insights on RNA folding mechanisms from catalytic RNA
Cellular and Molecular Life Sciences (CMLS), 2000 Methods for probing RNA structure in real time have revealed that initial folding steps are complete in less than a second. Refolding of large catalytic RNAs in vitro often results in long-lived intermediates that reach the native structure very slowly.
Sarah A. Woodson
semanticscholar +6 more sources
The catalytic mechanism of the RNA methyltransferase METTL3
eLife, 2023 The complex of methyltransferase-like proteins 3 and 14 (METTL3-14) is the major enzyme that deposits N6-methyladenosine (m6A) modifications on messenger RNA (mRNA) in humans.
Ivan Corbeski +11 more
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Artificial self-cleaving molecules consisting of a tRNA precursor and the catalytic RNA of RNase P [PDF]
Nucleic Acids Research, 1993 We synthesized two types of chimeric RNAs between the catalytic RNA subunit of RNase P from Escherichia coli (M1 RNA) and a tRNA precursor (pre-tRNA); one had pre-tRNA at the 3' side to the M1 RNA (M1 RNA-pre-tRNA). The second had pre-tRNA at the 5' side
Yo Kikuch +2 more
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Small catalytic RNA: Structure, function and application [PDF]
, 1991 We have utilized a combination of photochemical cross-linking techniques and site-directed mutagenesis to obtain secondary and tertiary structure information for the self-cleaving, self-ligating subsequence of RNA from the negative strand of Satellite Tobacco Ringspot Virus.
J.A. Monforte
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Cross-Catalytic Replication of an RNA Ligase Ribozyme [PDF]
Chemistry & Biology, 2004 A self-replicating RNA ligase ribozyme was converted to a cross-catalytic format whereby two ribozymes direct each other's synthesis from a total of four component substrates. Each ribozyme binds two RNA substrates and catalyzes their ligation to form the opposing ribozyme.
Dong‐Eun Kim, Gerald F. Joyce
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RNA ligase ribozymes with a small catalytic core
Scientific Reports, 2023 Catalytic RNAs, or ribozymes, catalyze diverse chemical reactions that could have sustained primordial life in the hypothetical RNA world. Many natural ribozymes and laboratory evolved ribozymes exhibit efficient catalysis mediated by elaborate catalytic
Yoko Nomura, Yohei Yokobayashi
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An Efficient Catalytic DNA that Cleaves L-RNA. [PDF]
PLoS ONE, 2015 Many DNAzymes have been isolated from synthetic DNA pools to cleave natural RNA (D-RNA) substrates and some have been utilized for the design of aptazyme biosensors for bioanalytical applications.
Kha Tram +3 more
doaj +5 more sources