RNA-catalyzed evolution of catalytic RNA. [PDF]
Proc Natl Acad Sci U S AAn RNA polymerase ribozyme that was obtained by directed evolution can propagate a functional RNA through repeated rounds of replication and selection, thereby enabling Darwinian evolution. Earlier versions of the polymerase did not have sufficient copying fidelity to propagate functional information, but a new variant with improved fidelity can ...
Papastavrou N, Horning DP, Joyce GF.
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Mechanisms of catalytic RNA molecules. [PDF]
Biochem Soc Trans, 2021 Ribozymes are folded catalytic RNA molecules that perform important biological functions. Since the discovery of the first RNA with catalytic activity in 1982, a large number of ribozymes have been reported. While most catalytic RNA molecules act alone, some RNA-based catalysts, such as RNase P, the ribosome, and the spliceosome, need protein ...
Alonso D, Mondragón A.
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RRP6 from Trypanosoma brucei: crystal structure of the catalytic domain, association with EAP3 and activity towards structured and non-structured RNA substrates. [PDF]
PLoS ONE, 2014 RRP6 is a 3'-5' exoribonuclease associated to the eukaryotic exosome, a multiprotein complex essential for various RNA processing and degradation pathways.
Rosicler L Barbosa +5 more
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Crystal structures and snapshots along Tpt1-catalyzed phosphate transfer from nucleic acid to NAD+ [PDF]
Nature CommunicationsTpt1/TRPT1/KptA family proteins are evolutionarily conserved in all three domains of life. In fungi and plants, Tpt1 transfers 2’-PO4 2- from tRNA splice junction to NAD+, which is the final step of tRNA maturation and is critical for the function of ...
Chulei Cao +17 more
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NSUN7 is a catalytically inactive RNA m5C methyltransferase essential for sperm flagellum assembly
Nature CommunicationsRNA 5-methylcytosine (m5C) is a widespread RNA modification mainly catalyzed by the NSUN family, whose dysfunction is linked to various diseases. Among NSUN1-7, all possessing motifs IV and VI essential for RNA m5C formation, NSUN7’s catalytic activity ...
Jing Li +10 more
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The discovery of catalytic RNA [PDF]
Nature Reviews Molecular Cell Biology, 2017 John Abelson
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The discovery of a catalytic RNA within RNase P and its legacy. [PDF]
J Biol ChemKirsebom LA, Liu F, McClain WH.
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Molecular trade-offs in RNA ligases affected the modular emergence of complex ribozymes at the origin of life [PDF]
Royal Society Open Science, 2017 In the RNA world hypothesis complex, self-replicating ribozymes were essential. For the emergence of an RNA world, less is known about the early processes that accounted for the formation of complex, long catalysts from small passively formed molecules ...
Nisha Dhar +3 more
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Evidence for a non-catalytic ion-binding site in multiple RNA-dependent RNA polymerases. [PDF]
PLoS ONE, 2012 A high-affinity divalent cation-binding site located proximal to the catalytic center has been identified in several RNA-dependent RNA polymerases (RdRps), but the characteristics of such a site have not been systematically studied.
Heli A M Mönttinen +2 more
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Multiplexed RNA profiling by regenerative catalysis enables blood-based subtyping of brain tumors
Nature Communications, 2023 Current technologies to subtype glioblastoma (GBM), the most lethal brain tumor, require highly invasive brain biopsies. Here, we develop a dedicated analytical platform to achieve direct and multiplexed profiling of circulating RNAs in extracellular ...
Yan Zhang +12 more
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