Results 1 to 10 of about 249,749 (166)
Reshaping of the substrate-binding pocket to improve the catalytic activity of the non-specific endonuclease from Pseudomonas fluorescens [PDF]
Frontiers in Bioengineering and BiotechnologyNon-specific endonuclease cleaves the phosphodiester bonds of DNA and RNA. In this study, the structure of the recombinant non-specific endonuclease from Pseudomonas fluorescens (PfNuc) was analyzed and engineered to improve the catalytic performance.
Jing-Yi Zhang +5 more
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Structural basis for protein-free catalysis by ribonuclease P ribozyme [PDF]
Nature CommunicationsRibonuclease P (RNase P) is an essential metallonuclease found in all three domains of life. However, the structural basis for the ancient RNase P RNA component acting alone as a ribozyme and catalytic metal-ion chemistry remains unknown.
Yun-Tzai Lee +9 more
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The catalytic mechanism of the RNA methyltransferase METTL3
eLife, 2023 The complex of methyltransferase-like proteins 3 and 14 (METTL3-14) is the major enzyme that deposits N6-methyladenosine (m6A) modifications on messenger RNA (mRNA) in humans.
Ivan Corbeski +11 more
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Structural insights into C-terminus-mediated RNA target cleavage by a mesophilic prokaryotic argonaute [PDF]
Nature CommunicationsProkaryotic Argonaute proteins (pAgos) are programmable nucleases that always utilize DNA guides to cleave DNA targets. Recent studies show that some pAgos preferentially utilize DNA guides to cleave RNA targets rather than DNA targets.
Taiyu Chen +11 more
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Evidence for a non-catalytic ion-binding site in multiple RNA-dependent RNA polymerases. [PDF]
PLoS ONE, 2012 A high-affinity divalent cation-binding site located proximal to the catalytic center has been identified in several RNA-dependent RNA polymerases (RdRps), but the characteristics of such a site have not been systematically studied.
Heli A M Mönttinen +2 more
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Multiplexed RNA profiling by regenerative catalysis enables blood-based subtyping of brain tumors
Nature Communications, 2023 Current technologies to subtype glioblastoma (GBM), the most lethal brain tumor, require highly invasive brain biopsies. Here, we develop a dedicated analytical platform to achieve direct and multiplexed profiling of circulating RNAs in extracellular ...
Yan Zhang +12 more
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Catalytic promiscuity in the RNA World may have aided the evolution of prebiotic metabolism.
PLoS Computational Biology, 2021 The Metabolically Coupled Replicator System (MCRS) model of early chemical evolution offers a plausible and efficient mechanism for the self-assembly and the maintenance of prebiotic RNA replicator communities, the likely predecessors of all life forms ...
Dániel Vörös +2 more
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F1000Research, 2016 Since the discovery of the first catalytic RNA in 1981, the field of ribozyme research has developed from the discovery of catalytic RNA motifs in nature and the elucidation of their structures and catalytic mechanisms, into a field of engineering and ...
Sabine Müller +4 more
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Frontiers in Chemistry, 2022 Three protein targets from SARS-CoV-2, the viral pathogen that causes COVID-19, are studied: the main protease, the 2′-O-RNA methyltransferase, and the nucleocapsid (N) protein. For the main protease, the nucleophilicity of the catalytic cysteine C145 is
Suhasini M. Iyengar +6 more
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Modulation of RNase E activity by alternative RNA binding sites.
PLoS ONE, 2014 Endoribonuclease E (RNase E) affects the composition and balance of the RNA population in Escherichia coli via degradation and processing of RNAs. In this study, we investigated the regulatory effects of an RNA binding site between amino acid residues 25
Daeyoung Kim +8 more
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