Structural and biochemical basis for DNA and RNA catalysis by human Topoisomerase 3β
Nature Communications, 2022 The authors revealed novel roles of catalytic residues and divalent metal ions for hsTOP3B, the human RNA topoisomerase, and demonstrated the structural elements that kinetically modulate the DNA and RNA topoisomerase activities of TOP3B.
Xi Yang +4 more
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RRP6 from Trypanosoma brucei: crystal structure of the catalytic domain, association with EAP3 and activity towards structured and non-structured RNA substrates. [PDF]
PLoS ONE, 2014 RRP6 is a 3'-5' exoribonuclease associated to the eukaryotic exosome, a multiprotein complex essential for various RNA processing and degradation pathways.
Rosicler L Barbosa +5 more
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RNA-catalyzed evolution of catalytic RNA
Proceedings of the National Academy of SciencesAn RNA polymerase ribozyme that was obtained by directed evolution can propagate a functional RNA through repeated rounds of replication and selection, thereby enabling Darwinian evolution. Earlier versions of the polymerase did not have sufficient copying fidelity to propagate functional information, but a new variant with improved fidelity can ...
Nikolaos Papastavrou +2 more
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An Efficient Catalytic DNA that Cleaves L-RNA. [PDF]
PLoS ONE, 2015 Many DNAzymes have been isolated from synthetic DNA pools to cleave natural RNA (D-RNA) substrates and some have been utilized for the design of aptazyme biosensors for bioanalytical applications.
Kha Tram +3 more
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Structural insights into the molecular mechanism of the m6A writer complex
eLife, 2016 Methylation of adenosines at the N6 position (m6A) is a dynamic and abundant epitranscriptomic mark that regulates critical aspects of eukaryotic RNA metabolism in numerous biological processes.
Paweł Śledź, Martin Jinek
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Mutational analysis of Trypanosoma brucei RNA editing ligase reveals regions critical for interaction with KREPA2. [PDF]
PLoS ONE, 2015 The Trypanosoma brucei parasite causes the vector-borne disease African sleeping sickness. Mitochondrial mRNAs of T. brucei undergo posttranscriptional RNA editing to make mature, functional mRNAs.
Vaibhav Mehta +3 more
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Scientific Reports, 2019 Regulation of gene expression through processing and turnover of RNA is a key mechanism that allows bacteria to rapidly adapt to changing environmental conditions.
Charlotte E. Mardle +7 more
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Ratcheted transport and sequential assembly of the yeast telomerase RNP
Cell Reports, 2023 Summary: The telomerase ribonucleoprotein particle (RNP) replenishes telomeric DNA and minimally requires an RNA component and a catalytic protein subunit.
Hannah Neumann +3 more
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Ribozyme biotechnology: emerging opportunities for medical applications in the RNA therapeutics era
Medical ReviewRNA-based medicines have transformed modern therapeutics, exemplified by the clinical success of small interfering RNAs (siRNAs), antisense oligonucleotides (ASOs), and mRNA vaccines.
Zhang Jingjing +7 more
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In vivo RNA targeting in the nematode Caenorhabditis elegans using exogenous catalytic DNA
BioTechniquesCatalytic DNA molecules (DNAzymes) offer a programmable means to manipulate RNA without genomic modification. Although DNAzymes have been thoroughly characterized in culture and in various animals, their utility in the prevalent model system ...
Eriс Khananshvilli +5 more
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