Results 31 to 40 of about 253,526 (264)

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Ratcheted transport and sequential assembly of the yeast telomerase RNP

Cell Reports, 2023
Summary: The telomerase ribonucleoprotein particle (RNP) replenishes telomeric DNA and minimally requires an RNA component and a catalytic protein subunit.
Hannah Neumann, Louise Bartle, Erin Bonnell, Raymund J. Wellinger   +3 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Ribozyme biotechnology: emerging opportunities for medical applications in the RNA therapeutics era

Medical Review
RNA-based medicines have transformed modern therapeutics, exemplified by the clinical success of small interfering RNAs (siRNAs), antisense oligonucleotides (ASOs), and mRNA vaccines.
Zhang Jingjing, Xi Zhiqin, Zhao Ming, Bai Jie, Li Zhenzhen, Zhan Xuelin, Zhang Ying, Liu Yijin   +7 more
doaj   +1 more source

In vivo RNA targeting in the nematode Caenorhabditis elegans using exogenous catalytic DNA

BioTechniques
Catalytic DNA molecules (DNAzymes) offer a programmable means to manipulate RNA without genomic modification. Although DNAzymes have been thoroughly characterized in culture and in various animals, their utility in the prevalent model system ...
Eriс Khananshvilli, Ayelet-Chen Abraham, Netanel Cohen, Michal Staum, Dorene Friedman, Ithai Rabinowitch   +5 more
doaj   +1 more source

RNA Specificity and Autoregulation of DDX17, a Modulator of MicroRNA Biogenesis

Cell Reports, 2019
Summary: DDX17, a DEAD-box ATPase, is a multifunctional helicase important for various RNA functions, including microRNA maturation. Key questions for DDX17 include how it recognizes target RNAs and influences their structures, as well as how its ATPase ...
Tri D. Ngo, Alexander C. Partin, Yunsun Nam   +2 more
doaj   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

Computed Energetics of Nucleotides in Spatial Ribozyme Structures: An Accurate Identification of Functional Regions from Structure

The Scientific World Journal, 2004
Ribozymes are functionally diverse RNA molecules with intrinsic catalytic activity. Multiple structural and biochemical studies are required to establish which nucleotide bases are involved in the catalysis.
Ivan Y. Torshin
doaj   +1 more source

What RNA World? Why a Peptide/RNA Partnership Merits Renewed Experimental Attention

Life, 2015
We review arguments that biology emerged from a reciprocal partnership in which small ancestral oligopeptides and oligonucleotides initially both contributed rudimentary information coding and catalytic rate accelerations, and that the superior ...
Charles W. Carter
doaj   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source