Results 101 to 110 of about 405,232 (337)

Comparative single‐cell transcriptomic profiling of patient‐derived renal carcinoma cells in cellular and animal models of kidney cancer

FEBS Open Bio, EarlyView.
We generated and characterized clear cell renal cell carcinoma models using the patient‐derived RCC243 cell line—including cell culture, orthotopic, and metastatic tumors—via single‐cell RNA‐sequencing for comparisons between models and patient tumor datasets.
Richard Huang, Lynn Kee, Alexander Gont, Jalna Meens, Fraser G. Ferens, Meredith S. Irwin, Laurie Ailles, Scott A. Yuzwa, Claire M. Robinson, Michael Ohh   +9 more
wiley   +1 more source

Negative Subtraction Hybridization: An efficient method to isolate large numbers of condition-specific cDNAs

BMC Genomics, 2004
Background The construction of cDNA libraries is a useful tool to understand gene expression in organisms under different conditions, but random sequencing of unbiased cDNA collections is laborious and can give rise to redundant EST collections. We aimed
Hall Leo T, Ayoubi Patricia, Macwana Sunita, Ray Anamika, Prade Rolf, Mort Andrew J   +5 more
doaj   +1 more source

Molecular cloning, expression, localisation and functional characterisation of a rabbit SULT1C2 sulfotransferase [PDF]

, 1999
The importance of sulfotransferases in xenobiotic metabolism is gaining recognition. The gastrointestinal (CI) tract is a major portal of entry for many xenobiotics, yet little is known about the contribution of sulfotransferases to detoxication or ...
Aksoy, Campbell, Foldes, Her, Her, Laemmli, Lowry, McManus, Mulder, Nagata, Nash, Ogura, Otterness, Ozawa, Reuben, Sandhu, Sanger, Short, Sundaram, Thorgeirsson, Towbin, Varin, Veronese, Weinshilboum, Weinshilboum, Yamazoe, Yoshinari, Yoshinari, Yoshinari, Zhu, Zhu   +30 more
core   +2 more sources

On subcellular distribution of the zinc finger 469 protein (ZNF469) and observed discrepancy in the localization of endogenous and overexpressed ZNF469

FEBS Open Bio, EarlyView.
ZNF469 regulates the expression of genes encoding extracellular matrix proteins. Endogenous ZNF469 is predominantly cytoplasmic, while in transfected cells, it forms aggregates reminiscent of biomolecular condensates, located mainly in the nucleus. These condensates exhibit overlapping staining with proteasomes and are also associated with the mitotic ...
Anne Elisabeth Christensen Mellgren, Ileana Cristea, Thomas Stevenson, Endy Spriet, Per Morten Knappskog, Stig Ove Bøe, Harald Kranz, Sushma N. Grellscheid, Eyvind Rødahl   +8 more
wiley   +1 more source

Hepatitis B virus X protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding protein

Virology Journal, 2005
Background To isolate cellular proteins interacting with hepatitis B virus X protein (HBX), from HepG2 cells infected with hepatitis B virus (HBV).
Chen Wei, Lwa Siew
doaj   +1 more source

The cDNA-deduced primary structure of human sex hormone-binding globulin and location of its steroid-binding domain [PDF]

, 1987
We have sequenced a cDNA for sex hormone-binding globulin (SHBG) isolated from a phage λgt 11 human liver cDNA library. The library was screened with a radiolabeled rat androgen-binding protein (ABP) cDNA, and the abundance of SHBG cDNAs was 1 in 750 000
Bardin, C.W., Cheng, C.Y., Goping, I.S., Hammond, G.L., Harley, M.J., Musto, N.A., Smith, C.L., Underhill, D.A.   +7 more
core   +1 more source

Translating Muscle RNAseq Into the Clinic for the Diagnosis of Muscle Diseases

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Approximately half of patients with hereditary myopathies remain without a definitive genetic diagnosis after DNA next‐generation sequencing (NGS). Here, we implemented transcriptome analysis of muscle biopsies as a complementary diagnostic tool for patients with muscle disease but no definitive genetic diagnosis after exome ...
Alba Segarra‐Casas, Cristina Domínguez‐González, Daniel Natera‐de Benito, Solange Kapetanovic, Aurelio Hernández‐Laín, Berta Estévez‐Arias, Laura Llansó, Carlos Ortez, Cristina Jou, Itxaso Martí‐Carrera, Arístides López‐Márquez, Maria José Rodríguez, Laura González‐Mera, Velina Nedkova, Roberto Fernández‐Torrón, Benjamín Rodríguez‐Santiago, Cecília Jimenez‐Mallebrera, Raul Juntas‐Morales, Adolfo López‐de Munain, Jordi Surrallés, Andrés Nascimento, Eduard Gallardo, Montse Olivé, Pia Gallano, Lidia González‐Quereda   +24 more
wiley   +1 more source

SMART amplification combined with cDNA size fractionation in order to obtain large full-length clones

BMC Genomics, 2004
Background cDNA libraries are widely used to identify genes and splice variants, and as a physical resource for full-length clones. Conventionally-generated cDNA libraries contain a high percentage of 5'-truncated clones.
Poustka Annemarie, Schupp Ingo, Wellenreuther Ruth, Wiemann Stefan   +3 more
doaj   +1 more source

Gene expression during preimplantation mouse development [PDF]

, 1992
To develop a resource for the identification and isolation of genes expressed in the early mammalian embryo, large and representative cDNA libraries were constructed from unfertilized eggs, and two-cell, eight-cell, and blastocyst-stage mouse embryos ...
DeLoia, J. A., Johnson, D., Knowles, B., Rothstein, J. L., Skowronski, J., Solter, D.   +5 more
core   +1 more source

Retrotransposon Expression Is Upregulated in Adulthood and Suppressed during Regeneration of the Limb in the Axolotl (Ambystoma mexicanum)

Advanced Biology, EarlyView.
The axolotl's remarkable regenerative abilities decline with age, the causes may include the numerous repetitive elements within its genome. This study uncovers how Ty3 retrotransposons and coexpression networks involving muscle and immune pathways respond to aging and regeneration, suggesting that transposons respond to physiological shifts and may ...
Samuel Ruiz‐Pérez, Nicolás Alcaraz, Karla Torres‐Arciga, José Antonio Ocampo‐Cervantes, Alejandra Cervera, Clementina Castro‐Hernández, Cynthia Gabriela Sámano‐Salazar, Ernesto Soto‐Reyes, Rodrigo González‐Barrios   +8 more
wiley   +1 more source