Results 111 to 120 of about 405,232 (337)

Excess primer degradation by Exo I improves the preparation of 3′ cDNA ligation-based sequencing libraries

BioTechniques, 2019
RNA sequencing library construction using single-stranded ligation of a DNA adapter to 3′ ends of cDNAs often produces primer–adapter byproducts, which compete with cDNA–adapter ligation products during library amplification and, therefore, reduces the ...
Christel Hougård Enroth, Annaleigh Ohrt Fehler, Line Dahl Poulsen, Jeppe Vinther   +3 more
doaj   +1 more source

HMGB1 Derived from the Pyroptotic Microenvironment Promotes Macrophage Extracellular Traps in Hirschsprung‐Associated Enterocolitis

Advanced Biology, EarlyView.
HMGB1 derived from the pyroptotic environment in Hirschsprung‐associated enterocolitis mediates the formation of macrophage extracellular traps through TLR4 ‐p38 MAPK/p65 NF‐kB signaling pathways. Macrophage extracellular traps induce increased ROS production and pyroptosis of colonic epithelial cells.
Rui Zhang, Jing Li, Lili Song, Liya Pan, Chengchen Zhang, Zhiyan Zhan, Li Hong   +6 more
wiley   +1 more source

cDNA Library Screening Using the SOS Recruitment System

BioTechniques, 2001
The SOS recruitment system (SRS), a recently developed method for detecting protein-protein interactions, provides an attractive alternative to identify biologically important protein interactions.
Wendong Huang, Sung-ling Wang, Guillermina Lozano, Benoit de Crombrugghe   +3 more
doaj   +1 more source

Novel Biologically Active Glass Fiber Functionalized Using Magnesium Phosphate Cement Promotes Bone and Vascular Regeneration

Advanced Biology, EarlyView.
In this study, a new type of bioactive glass fiber ‐based composite magnesium phosphate bone cement is prepared and verified that its mechanical strength and biological properties. In addition, the cement may have played a biologically active role in the Notch and HIF signaling pathways.
Yuzheng Lu, Yanbo Shan, Yingjie Xiong, Jianting Ye, Yanbin Wu, Jipeng Song, Yao Zhang, Wancheng Lin, Haoye Meng, Wenjing Xu, Jiang Peng, Qiang Lu, Lixiang Ding   +12 more
wiley   +1 more source

Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags

Memorias do Instituto Oswaldo Cruz, 2007
The characterization of expressed sequence tags (ESTs) generated from a cDNA library of Leishmania (Leishmania) amazonensis amastigotes is described. The sequencing of 93 clones generated new L. (L.) amazonensis ESTs from which 32% are not related to any
Luciana Girotto Gentil, Fernanda Lasakosvitsch, José Franco da Silveira, Márcia Regina Machado dos Santos, Clara Lúcia Barbiéri   +4 more
doaj   +1 more source

The Molecular Composition of Peptide Toxins in the Venom of Spider Lycosa coelestis as Revealed by cDNA Library and Transcriptomic Sequencing. [PDF]

Toxins (Basel), 2023
Wu X, Chen Y, Liu H, Kong X, Liang X, Zhang Y, Tang C, Liu Z.   +7 more
europepmc   +1 more source

Molecular cloning and functional expression of a novel brain-specific inward rectifier potassium channel [PDF]

, 1994
We have cloned a novel brain-specific inward rectifier K+ channel from a mouse brain cDNA library and designated it MB-IRK3. The mouse brain cDNA library was screened using a fragment of the mouse macrophage inward rectifier K+ channel (IRK1) cDNA as a ...
Jahangir, Arshad, Koyama, Hidekazu, Kurachi, Yoshihisa, Morishige, Ken-Ichirou, Takahashi, Naohiko, Yamada, Mitsuhiko, Zanelli, Jill S.   +6 more
core   +1 more source

Consolidate Overview of Ribonucleic Acid Molecular Dynamics: From Molecular Movements to Material Innovations

Advanced Engineering Materials, EarlyView.
Molecular dynamics simulations are advancing the study of ribonucleic acid (RNA) and RNA‐conjugated molecules. These developments include improvements in force fields, long‐timescale dynamics, and coarse‐grained models, addressing limitations and refining methods.
Kanchan Yadav, Iksoo Jang, Jong Bum Lee
wiley   +1 more source

In vivo gain-of-function cDNA library screening for colonization genes in a mouse model of pulmonary metastasis. [PDF]

STAR Protoc, 2022
Tu Z, Karnoub AE.
europepmc   +1 more source

Cloning of CRP2, a novel member of the cysteine-rich protein family with two repeats of an unusual LIM/double zinc-finger motif [PDF]

, 1993
The cDNA coding for a novel member of the cysteine-rich protein family was isolated from a rat brain cDNA library. It encodes a protein, denoted cysteine-rich protein 2 (CRP2), of 208 amino acid residues containing two tandem repeats of an unusual LIM ...
Kaji, Akira, Kimura, Toru, Mizuno, Kensaku, Nakamura, Toshikazu, Okano, Ichiro, Yamamoto, Takeshi   +5 more
core   +1 more source