This study integrates dual‐reporter genetics, imaging flow cytometry, and single‐cell sequencing to characterize rare bihormonal cells in mouse and human pancreatic islets. Gcg⁺Ins⁺ cells resemble α‐cells rather than transitional states. Cross‐species gene network analysis refines islet cell taxonomy and reveals human‐specific δ‐cell subtypes ...
Xin‐Xin Yu+10 more
wiley +1 more source
Construction of cDNA library of Dalbergia odorifera induced by low temperature stress and screening of low temperature tolerant genes. [PDF]
Li S, An X, Li F, Chen Y, Li X.
europepmc +1 more source
Comparative evaluation of cDNA library construction approaches for RNA-Seq analysis from low RNA-content human specimens. [PDF]
Masters TL+11 more
europepmc +1 more source
Simplified Protocol of Solid-Phase cDNA Libraries for Multiple PCR Amplification
Florence Fellmann+2 more
openalex +1 more source
A Large Scale Analysis of cDNA in Arabidopsis thaliana: Generation of 12,028 Non-redundant Expressed Sequence Tags from Normalized and Size-selected cDNA Libraries [PDF]
E. Asamizu
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Collaborative Duality of CircGLIS3(2) RNA and Protein in human Wound Repair
CircGLIS3(2), a circRNA transiently upregulated in dermal fibroblasts during acute wound healing, is induced by IL‐1α and TGF‐β. Its RNA activates fibroblasts into matrix‐secreting cells, while its encoded protein enhances proliferation. The RNA stabilizes PCOLCE, and the protein interacts with BTF3, collectively accelerating wound repair. Abstract The
Guanglin Niu+20 more
wiley +1 more source
Improved precision, sensitivity, and adaptability of ordered two-template relay cDNA library preparation for RNA sequencing. [PDF]
Ferguson L+5 more
europepmc +1 more source
Large-Scale Investigation of Soybean Gene Functions by Overexpressing a Full-Length Soybean cDNA Library in Arabidopsis. [PDF]
Li X+8 more
europepmc +1 more source
An improved method for generating subtracted cDNA libraries using phage lambda vectors
H. Hesse+2 more
openalex +2 more sources
Systematic identification of mutations that constitutively activate the angiotensin II type 1A receptor by screening a randomly mutated cDNA library with an original pharmacological bioassay [PDF]
Charles Parnot+5 more
openalex +1 more source