Results 301 to 310 of about 405,232 (337)
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2013
The construction of full-length cDNA libraries allows researchers to study gene expression and protein interactions and undertake gene discovery. Recent improvements allow the construction of high-quality cDNA libraries, with small amounts of mRNA. In parallel, these improvements allow for the incorporation of adapters into the cDNA, both at the 5' and
Maarten Kooiker, Gang-Ping Xue
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The construction of full-length cDNA libraries allows researchers to study gene expression and protein interactions and undertake gene discovery. Recent improvements allow the construction of high-quality cDNA libraries, with small amounts of mRNA. In parallel, these improvements allow for the incorporation of adapters into the cDNA, both at the 5' and
Maarten Kooiker, Gang-Ping Xue
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Preserving primary cDNA libraries
Analytical Biochemistry, 1987A technique for the long term storage of primary cDNA libraries in a form such that relevant DNA sequences can be readily identified and retrieved is described. cDNA libraries produced using the lambda gt 10 cloning vector were plated out on host bacteria in 0.7% top agarose supplemented with 30% glycerol.
Dennis M. Klinman, David I. Cohen
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1996
cDNA Library Construction from Small Amounts of RNA Using Paramagnetic Beads and PCR Kris N. Lambert and Valerie M. Williamson Increasing the Average Abundance of Low-Abundance cDNAs by Ordered Subdivision of cDNA Populations David R. Sibson and Michael P.
Caroline A. Austin, Ian G. Cowell
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cDNA Library Construction from Small Amounts of RNA Using Paramagnetic Beads and PCR Kris N. Lambert and Valerie M. Williamson Increasing the Average Abundance of Low-Abundance cDNAs by Ordered Subdivision of cDNA Populations David R. Sibson and Michael P.
Caroline A. Austin, Ian G. Cowell
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Subtractive Hybridization of cDNA Libraries
2003The technique of subtractive hybridization is used to enrich abundant cDNAs that differ between two cell populations. This approach is well suited for systems in which a homogenous cell population is activated in culture with an agent or factor that induces the transcription of genes that are either not present or at low abundance in the unactivated ...
Richard A. Maki, Gregory S. Kelner
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Constructing and detecting a cDNA library for mites
Parasitology Research, 2015RNA extraction and construction of complementary DNA (cDNA) library for mites have been quite challenging due to difficulties in acquiring tiny living mites and breaking their hard chitin. The present study is to explore a better method to construct cDNA library for mites that will lay the foundation on transcriptome and molecular pathogenesis research.
YaE Zhao +5 more
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Trends in Biotechnology, 2001
A collaboration between the Riken Genome Exploration Research Group Phase II team and the FANTOM Consortium has produced a collection of full-length complementary DNAs, their sequences and their physical mapping to the mouse genome (Nature, 8 February).
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A collaboration between the Riken Genome Exploration Research Group Phase II team and the FANTOM Consortium has produced a collection of full-length complementary DNAs, their sequences and their physical mapping to the mouse genome (Nature, 8 February).
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Production of a Subtracted cDNA Library
Current Protocols in Molecular Biology, 2001AbstractFor some experiments, a complete cDNA library is unnecessary and instead, a subtracted cDNA library is useful. A subtracted cDNA library contains cDNA clones corresponding to mRNAs present in one cell or tissue type and not present in a second type.
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cDNA libraries from identified neurons
Proceedings of the Royal Society of London. Series B: Biological Sciences, 1996One approach to studying the changes in gene expression which underlie differentiation is to construct cDNA libraries from different tissues or at different stages of development. However, generating representative cDNA libraries from heterogeneous tissues such as the nervous system is often a real problem.
Kim Kaiser +3 more
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Antibody-Screening cDNA Libraries
1985Molecular cloning and ancillary techniques offer an attractive alternative in studying many biological phenomena. Successful molecular cloning of genes encoding proteins for which amino acid sequence data are not available can be achieved by antibody (Ab) screening.
Denis R. Headon +6 more
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Construction of cDNA Libraries in Vaccinia Virus
2004Poxvirus expression vectors have gained widespread use for expression of foreign proteins and as delivery vehicles for vaccine antigens. We have developed a novel method using the poxvirus as a library vector for functional selection of specific cDNA. Poxviruses have several unique and useful properties as a library vector.
Shuying Shi +2 more
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