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1999
cDNA libraries are collections of recombinant DNA sequences, derived from and representing entire mRNA populations present at a given stage of development or differentiation in certain tissues or cell types. cDNA libraries can be self-made and tailored according to individual experimental needs.
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cDNA libraries are collections of recombinant DNA sequences, derived from and representing entire mRNA populations present at a given stage of development or differentiation in certain tissues or cell types. cDNA libraries can be self-made and tailored according to individual experimental needs.
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Olfactory-like receptor cDNAs are present in human lingual cDNA libraries
Biochemical and Biophysical Research Communications, 2005Olfactory and pheromone receptors (ORs) constitute a large family of G-protein-coupled receptors involved in the detection and transduction of odorant signals. Using degenerated primers complementary to the highly conserved transmembrane domains II, III, VI, and VII within this protein family, Gaudin et al.
Durzyński, Łukasz +5 more
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Screening of cDNA Libraries on Glass Slide Microarrays [PDF]
, 2007 A quantitative screening method was developed to evaluate the quality of cDNA libraries constructed by suppression subtraction hybridization (SSH) or other enrichment techniques. The SSH technique was adapted to facilitate screening of the resultant library on a small number of glass slide microarrays.
Berger, Dave K +3 more
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Rapid screening of pseudorabies virus-specific cDNAs from a cDNA library
Journal of Virological Methods, 1996In order to reduce the time and cost for screening of pseudorabies virus (PRV)-specific cDNAs, a rapid and inexpensive method was developed that involved subtractive hybridization of the plasmid, which contained cDNA fragment, to PRV genomic DNA which was bound to nylon membranes.
Kay Wu +3 more
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Construction of cDNA Libraries in λgt10 or λgt11
2003To obtain a cDNA clone of an mRNA, the mRNA must be copied faithfully into DNA, and the cDNA library must be large enough to represent the abundance class that contains the mRNA of interest. For example, in tobacco, Goldberg (1) has shown that it is possible to divide the mRNA population into three classes with most of the mRNAs (11,300) being in the ...
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Constructing and screening cDNA libraries
1997The in-depth study of eukaryotic gene structure and expression has been made possible by the ability to clone transcribed DNA. Since RNA per se cannot be cloned (i.e. stably introduced and replicated in a bacterial cell) and RNA-DNA hybrids can only be cloned with very low efficiency, the best choice for the isolation of mRNA is its conversion into ...
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[13] Construction and analysis of arrayed cDNA libraries
1999For any attempt to understand the biology of an organism the incorporation of a cDNA-based approach is unavoidable, because it is a major approach to studying gene function. The complete sequence of the genome alone is not sufficient to understand any organism; its gene regulation, expression, splice variation, posttranslational modifications, and ...
Georgia D. Panopoulou +4 more
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Expression Screening of cDNA Libraries in pEX
2003Most methods for screening libraries of cDNA clones rely on the selective binding of either nucleic acid probes to cDNA molecules or antibodies to the polypeptide gene product encoded by the cDNA. Although screening of cDNA libraries with synthetic oligonucleotide probes is undoubtedly a powerful procedure, it is not always the easiest way to clone a ...
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Immunoscreening λgt11 cDNA Library
1989Publisher Summary This chapter discusses bacteriophage λgt11 that makes it possible to recognize a complementary DNA clone (cDNA). Bacteriophage λgt11 is an extremely useful cloning vector that can cause its bacterial host to express a foreign protein.
Thomas O. Brock, John S. Kizer
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Current Protocols in Molecular Biology, 1988
AbstractThis unit provides a basic description of issues that require careful consideration before undertaking construction of cDNA libraries. Topics include: generating a double‐stranded DNA copy of the mRNA, the quality of the mRNA, the relative abundance of the clone of interest, the choice of screening method, the size of the library that will be ...
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AbstractThis unit provides a basic description of issues that require careful consideration before undertaking construction of cDNA libraries. Topics include: generating a double‐stranded DNA copy of the mRNA, the quality of the mRNA, the relative abundance of the clone of interest, the choice of screening method, the size of the library that will be ...
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