Results 141 to 150 of about 2,073 (157)
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Detection of domoic acid in rat serum and brain by direct competitive enzyme-linked immunosorbent assay (cELISA)

Analytical and Bioanalytical Chemistry, 2005
In 1987 a large-scale incident of human poisoning in Canada was traced to commercial mussels contaminated with domoic acid (DOM). Since then, routine screening of shellfish domoic acid content has been carried out using a variety of assays, with liquid chromatography using ultraviolet absorbance detection (LC-UV) or mass spectrometric detection (LC-MS)
Blair R, Hesp   +4 more
openaire   +2 more sources

Cellular enzyme-linked immunospecific assay (CELISA). IV. Inhibition of endogenous cellular alkaline phosphatase activity

Journal of Immunological Methods, 1984
The cellular enzyme-linked immunospecific assay (CELISA) uses ligands conjugated to calf intestinal alkaline phosphatase to quantitate antibody bound to antigens on the surfaces of whole cells. Since the endogenous alkaline phosphatase present in many cell types contributes unwanted background noise to the assay, ways of inhibiting endogenous cellular ...
R E, Morris, D A, Horowitz
openaire   +2 more sources

Standardization of equine infectious anemia immunodiffusion and celisa tests and their application to control of the disease in the united states

Journal of Equine Veterinary Science, 1988
Summary The United States (US) program to control equine infectious anemia (EIA) has been based on restrictions on the movement of immunodiffusion (ID) test-positive horses and a requirement for a negative test prior to interstate movement by 42 states. The slaughter of positive horses has not been required.
J.E. Pearson, C.A. Gipson
openaire   +1 more source

TIPS AND STEP-BY-STEP PROTOCOL FOR THE OPTIMIZATION OF IMPORTANT FACTORS AFFECTING CELLULAR ENZYME-LINKED IMMUNOSORBENT ASSAY (CELISA)

Journal of Immunoassay and Immunochemistry, 2001
CELISA, or cellular enzyme-linked immunosorbent assay, is a powerful and easy to use technique to study cell surface antigens under different stimulations. Nevertheless, some factors must be discussed and optimized prior to reaching a reproducible CELISA.
G Ghanem, R Morandini
exaly   +4 more sources

A simple and rapid competitive enzyme-linked immunosorbent assay (cELISA) for high-throughput measurement of secretory immunoglobulin A (sIgA) in saliva

Talanta, 2010
A simple competitive enzyme-linked immunosorbent assay (cELISA) was established for rapid measurement of secretory immunoglobulin A (sIgA) in saliva. The method was based on competitive reaction between the immobilized IgA and free IgA in the solution for the limited amount of horseradish peroxidase-conjugated rabbit anti-human IgA.
Dan, Wang   +2 more
exaly   +3 more sources

Identification and quantification of the toxic dinoflagellate Gymnodinium sp. with competitive enzyme-linked immunosorbent assay (cELISA)

Harmful Algae, 2005
Abstract Polyclonal antibodies were raised against Gymnodinium sp. by immunizing rabbits with cells of the axenic strain. Based on the species-specific antiserum, an indirect competitive enzyme-linked immunosorbent assay (cELISA) was developed to identify and quantify Gymnodinium sp. A standard curve was established to correlate the cELISA signal
ZeYu Xin   +7 more
openaire   +1 more source

Cellular enzyme-linked immunospecific assay (CELISA). I a new micromethod that detects antibodies to cell-surface antigens

Human Immunology, 1982
A novel, indirect immunoassay capable of detecting human anti-HLA antibody bound to lymphocytes has been developed. This cellular enzyme-linked immunospecific assay (CELISA) utilizes an antiglobulin covalently linked to alkaline phosphatase to quantitate the amount of anti-HLA antibody bound to cell-surface HLA antigens.
R E, Morris, P T, Thomas, R, Hong
openaire   +2 more sources

Cellular enzyme-linked immunospecific assay (CELISA). III. Use of the CELISA to quantitate monoclonal antibodies bound to HLA antigens and to subset-specific antigens on cell surfaces.

Transplantation, 1984
The cellular enzyme-linked immunospecific assay (CELISA) quantitates the binding of antibody to cell-surface antigens. When conditions that produced the maximum specific enzymatic activity of the conjugate were used, the CELISA was able to screen hybridoma supernatant fluids for polymorphic anti-HLA monoclonal antibodies using as few as 20,000 normal ...
openaire   +1 more source

Development of a cELISA based on a trivalent nanobody–HRP fusion protein targeting BP26 for brucellosis diagnosis

Talanta
Brucellosis is a zoonotic disease that poses a serious threat to both humans and animals, not only creating a significant public health risk but also causing considerable economic losses. Timely and accurate diagnosis is critical for the effective prevention and control of brucellosis.
Gaowa WuDong   +11 more
openaire   +2 more sources

Anti-paternal lymphocyte antibodies: a modified cellular enzyme-linked immunospecific assay (CELISA).

Medical laboratory sciences, 1992
A cellular enzyme-linked immunospecific assay is described which is ideally suited to measuring the IgG antibody response to donor-specific lymphocyte infusions. The procedure is simple, sensitive, specific and objective.
D C, Kilpatrick   +2 more
openaire   +1 more source

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