Results 1 to 10 of about 1,478,797 (154)

Development and Application of a Nanobody-Based Competitive ELISA for Detecting Antibodies against Hepatitis E Virus from Humans and Domestic Animals

Microbiology Spectrum, 2023
Hepatitis E virus (HEV) is a zoonotic pathogen that is widespread worldwide. At present, most enzyme-linked immunosorbent assay (ELISA) kits only detect antibodies against human HEV.
En-Min Zhou, Qin Zhao, Baoyuan Liu
exaly   +4 more sources

Serological Detection of Antibodies Against Mycoplasma bovis Infection by Competitive Enzyme-Linked Immunosorbent Assay (cELISA) [PDF]

Veterinary Sciences
Mycoplasma bovis (M. bovis) is a major pathogen in cattle, primarily responsible for bovine respiratory disease complex (BRDC). Characterized by high infectivity and morbidity, M.
Wentao Fei, Li Yang, Yuhao Zhao, Zhijie Xiang, Chengwei Fang, Yingyu Chen, Changmin Hu, Aizhen Guo   +7 more
doaj   +3 more sources

Development of a Competitive ELISA for Detecting Antibodies Against Pseudorabies Virus Glycoprotein D [PDF]

Transboundary and Emerging Diseases
Pseudorabies virus (PRV) causes substantial economic losses in the global swine industry. Serological diagnosis plays a crucial role in its eradication. Here, we developed a competitive enzyme‐linked immunosorbent assay (cELISA) to detect antibodies against PRV glycoprotein D (gD).
Jinqiu Zhang, Yan-Dong Tang
exaly   +3 more sources

Development of a novel nanobody-based competitive ELISA for rapid detection of antibodies against Senecavirus A [PDF]

BMC Veterinary Research
Background Senecavirus A (SVA) causes considerable economic losses in the swine industry, particularly among piglets. However, effective serological diagnostic assays for SVA are still lacking. A testing platform based on nanobody-horseradish peroxidase (
Longzhi Nie, Tianxiang Chen, Yunpeng Shi, Bing Wu, Chao Xiong, Qin Zhao, Yuchen Nan, Yiyang Chen, Baoyuan Liu   +8 more
doaj   +2 more sources

Development and application of a NP-cELISA for the detection of nucleoprotein antibodies of equine influenza virus [PDF]

Microbiology Spectrum
Equine influenza (EI), caused by the equine influenza virus (EIV), is an acute respiratory disease that has become enzootic worldwide, resulting in frequent outbreaks and substantial economic losses within the equine industry. In this study, we developed
Yan Yang, Kui Guo, Ling Xu, Wei Guo, Mingqi Dong, Wen Liu, Shuaijie Li, Zenan Zhang, Xiaoyu Chu, Yaoxin Wang, Zhenyu Zhang, Zhe Hu, Xiaojun Wang   +12 more
doaj   +2 more sources

Optimisation and Scale-Up of the Heterologous Production of the Antifungal Protein PeAfpA From Penicillium expansum in Stirred-Tank Bioreactors. [PDF]

Microb Biotechnol
This work demonstrates the feasibility of scaling up laboratory processes to pilot scale production of antifungal proteins for diverse applications and exemplifies the possibilities that recyclable CRISPR/Cas9 methodology offers for fungal biotechnology.
de Ovalle S, Garrigues S, Gimeno-Alcañiz JV, Mercader JV, Abad-Fuentes A, Marcos JF, Manzanares P.   +6 more
europepmc   +2 more sources

Development of a nanobody-based competitive enzyme-linked immunosorbent assay for the sensitive detection of antibodies against porcine deltacoronavirus [PDF]

Journal of Clinical Microbiology
Porcine deltacoronavirus (PDCoV) is an emerging porcine enteric coronavirus causing significant economic losses to the pig farming industry globally.
Ruiming Yu, Liping Zhang, Yingjie Bai, Peng Zhou, Jun Yang, Dongsheng Wang, Liyang Wei, Zhongwang Zhang, Chenghua Yan, Yonglu Wang, Huichen Guo, Li Pan, Ligang Yuan, Xinsheng Liu   +13 more
doaj   +2 more sources

Development of a nucleocapsid protein-based competitive ELISA for the detection of porcine deltacoronavirus antibodies [PDF]

Frontiers in Microbiology
Porcine deltacoronavirus (PDCoV) is a significant threat to the global swine industry, causing severe diarrhea in piglets and exhibiting a broad host range with zoonotic potential. The nucleocapsid (N) protein of PDCoV is highly conserved and immunogenic,
Shuizhong Han, Shuizhong Han, Yu Zhou, Maiping Li, Penggang Chen, Xingming Huang, Ying Wang   +6 more
doaj   +2 more sources

A highly sensitive one-step nanobody-based immunoassay to specifically detect antibodies against fowl adenovirus serotype 4 [PDF]

Poultry Science
Hepatitis-hydropericardium syndrome, caused by fowl adenovirus serotype 4 (FAdV-4), has resulted in significant economic damage to the poultry industry.
Pinpin Ji, Hao Zhang, Xiri Yangzong, Ziling Li, Zhixiang Liu, Miao Dan, Xiaoxuan Li, Xuwen Sun, Qin Zhao, Yani Sun   +9 more
doaj   +2 more sources

Seroprevalence of Contagious Caprine Pleuropneumonia in Selected Districts of Borana Zone, Oromia Region, Southern Ethiopia. [PDF]

Vet Med Sci
A cross‐sectional study, conducted on contagious caprine pleuropneumonia (CCPP) in the goat population, revealed a seroprevalence of 15.85% (95% CI: 12.06%–20.54%). Undertaking a broader research involving a larger‐scale study and reservoir species, including sheep, is recommended to gain deeper practical insights into the disease.
Desa G, Benti T, Zewde D.
europepmc   +2 more sources