Development of Indirect Competitive ELISA and Visualized Multicolor ELISA Based on Gold Nanorods Growth for the Determination of Zearalenone [PDF]
In this study, a zearalenone (ZEN) hapten was designed and prepared against the mycotoxin ZEN, and the original coating ZEN-ovalbumin (ZEN-OVA) was prepared by conjugation with OVA. Based on the gold nanorods (AuNRs) of uniform size and stable properties
Tianyu Ma +5 more
doaj +4 more sources
Characterization of Competitive ELISA and Formulated Alhydrogel Competitive ELISA (FAcE) for Direct Quantification of Active Ingredients in GMMA-Based Vaccines [PDF]
Generalized modules for membrane antigens (GMMA) represent a technology particularly attractive for designing affordable vaccines against Gram-negative bacteria. We explored such technology for the development of O-antigen-based vaccines against Shigella
Omar Rossi +5 more
doaj +5 more sources
Establishment of a Direct Competitive ELISA for Camel FGF21 Detection [PDF]
Camels, with the ability to survive under drought and chronic hunger, developed exceptional efficient lipid reserves and energy substance metabolic characteristics.
Yuxuan Yang +7 more
doaj +4 more sources
Development of a competitive ELISA based on the LSDV A33 antigen [PDF]
Goatpoxvirus (GTPV), sheeppoxvius (SPPV), and the Lumpy skin disease virus (LSDV) is a Capripoxvirus belonging to the family poxviridae. They can cause significant economic losses in countries where this disease are endemic.
Guohua Chen +10 more
doaj +4 more sources
ELISA-based competitive trypsin inhibition assay [PDF]
Proteolytic enzymes are crucial for numerous biological processes and are tightly controlled by specific inhibitors. In addition to the research activities to investigate the natural effects of these inhibitors, it is important to determine the ...
Bengü Ergenoğlu +6 more
doaj +4 more sources
Establishment of an Indirect Competitive ELISA for Zearalenone
In this study, an indirect competitive ELISA (ic-ELISA) was developed for the detection of zearalenone in corn flour. The optimal working concentration of antigen and monoclonal antibody was determined by chessboard method.
Song TANG +7 more
doaj +2 more sources
A Monoclonal Antibody-Based Indirect Competitive ELISA for Detecting Goose Astrovirus Antibodies [PDF]
Goose astrovirus (GoAstV) infection has become prevalent in major goose-producing regions, causing substantial economic losses to the industry. In this study, an indirect competitive ELISA (ic-ELISA) was developed based on a monoclonal antibody (mAb ...
Junfeng Lv +6 more
doaj +2 more sources
Serodiagnosis of Przhevalskiana spp. Infestation in Goats Using a Competitive ELISA [PDF]
Goat warble-fly infestation is one of the parasitic diseases caused by the larvae of przhevalskiana spp. It is known to be a major challenge for health and welfare in infested goats and causes high economic losses in livestock worldwide. The detection of
A. Bagheri +3 more
doaj +2 more sources
A pan-genotypic indirect competitive ELISA for serological detection of pigeon circovirus antibodies [PDF]
Pigeon circovirus (PiCV) infection, which causes young pigeon disease syndrome (YPDS) and immunosuppression, significantly impacts both the meat and racing pigeon industries.
Weifan Wang +16 more
doaj +2 more sources
Reproducibility in the Quantification of mRNA Levels by RT-PCR-ELISA and RT Competitive-PCR-ELISA
The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps.
L.L. Hall +5 more
doaj +3 more sources

