Results 21 to 30 of about 53,639 (248)
Establishment of Indirect Competitive ELISA Method for Detecting Amanitin in Mushroom
Shipin gongye ke-ji, 2022 Objective: This study aims to establish an indirect competitive enzyme-linked immunosorbent assay for the detection of amanitin (AMA) in mushroom. Methods: In this study, the hapten was obtained by introducing 6-aminocaproic acid into the carboxyl ...
Hebing LIU +3 more
doaj +1 more source
Competitive ELISA for Protein-lipopolysaccharide (LPS) Binding
Bio-Protocol, 2014 Lipopolysaccharide is the major constituent of the outer membrane of gram-negative bacteria and, once released from the bacterial surface into the bloodstream, is a potent activator of the host immune system, which can lead to septic shock.
Victoria Martínez-Sernández +1 more
doaj +1 more source
Czech Journal of Food Sciences, 2016 We established and evaluated a polyclonal antibody based competitive enzyme-linked immunosorbent assay for the quantification of defensin-1 in honey. The assay showed an inhibitory concentration (IC50) value of 111.5 ± 15.41 ng/ml with a detection limit ...
Ivana Valachová +2 more
doaj +1 more source
Development of competitive ELISA for neosporosis by employing immunoproteomics [PDF]
Clinical Proteomics, 2004 In this study, proteomics was used to explore the antigenic proteins that are involved in cross-reactivity during serodiagnosis between Neospora caninum (N. caninum) and Toxoplasma gondii (T. gondii). Competitive enzyme-linked immunosorbent assay (C-ELISA) developed by proteomics shed a new light on the infection of N. caninum.
Shin, Yong-seung +11 more
openaire +1 more source
Anti-idiotypic VHH mediated environmentally friendly immunoassay for citrinin without mycotoxin
Food and Agricultural Immunology, 2020 Competitive enzyme-linked immunosorbent assay (ELISA) is the most widely used method for rapidly screening of mycotoxins in contaminated food products; however, it involves the use of toxin-protein conjugates as a competitive antigen and toxin standards ...
Liang Xiong +6 more
doaj +1 more source
Gluten Fragment Detection with a Competitive ELISA
Journal of AOAC INTERNATIONAL, 2012 Abstract The second generation of a competitive ELISA for prolamin quantification based on the R5 antibody was studied for method performance and suitability to detect partially hydrolyzed prolamins in food. To be able to convert signal intensities to gluten concentrations, as required by the Codex Alimentarius Standard, a new calibrator
Sigrid, Haas-Lauterbach +3 more
openaire +2 more sources
Competitive ELISA of Bovine Lactoferrin with Bispecific Monoclonal Antibodies [PDF]
Bioscience, Biotechnology, and Biochemistry, 1997 A mouse hybrid-hybridoma, HH1-4-3, secreting IgG1 class bispecific antibodies to bovine lactoferrin (bLF) and horseradish peroxidase (HRPO), was established previously. The competitive enzyme-linked immunosorbent assay (ELISA) of bLF using the HH1-4-3 culture supernatant was not sensitive enough to measure bLF concentration in biological fluids.
SHINMOTO, Hiroshi +3 more
openaire +2 more sources
TOXOCARIASIS: SEROLOGICAL DIAGNOSIS BY INDIRECT ANTIBODY COMPETITION ELISA [PDF]
Revista do Instituto de Medicina Tropical de São Paulo, 1999 Toxocariasis is caused by infection of man by Toxocara canis and Toxocara cati larvae, the common roundworm of dogs and cats. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. Non specific reactions are observed mainly due to cross-reactivity with Ascaris sp antigens.
Nunes, Caris Maroni +4 more
openaire +5 more sources
Journal of Pure and Applied Microbiology, 2019 The aim of the present study was to detect the prevalence of Anaplasma marginale in buffaloes in two Iraqi governorates, Al-Qadisiyah and Babylon, by the microscopy as well as the competitive-ELISA that used firstly among the Iraqi buffaloes.
Luma FM. Al-Ethafa +2 more
doaj +1 more source
Development and Validation of Competitive ELISA for Detection of H5 Hemagglutinin Antibodies
Poultry, 2023 Influenza A viruses (IAVs) belonging to the goose/Guangdong (Gs/GD)-lineage H5Nx remain a major concern for the global poultry industry, wildlife, and humans.
Orie Hochman +7 more
doaj +1 more source