Results 11 to 20 of about 53,639 (248)

Nanobody-Based Indirect Competitive ELISA for Sensitive Detection of 19-Nortestosterone in Animal Urine [PDF]

Biomolecules, 2021
Nanobody (Nb), a new type of biorecognition element generally from Camelidae, has the characteristics of small molecular weight, high stability, great solubility and high expression level in E. coli.
Yuan-yuan Yang, Yu Wang, Yi-feng Zhang, Feng Wang, Yi-fan Liang, Jin-yi Yang, Zhen-lin Xu, Yu-dong Shen, Hong Wang   +8 more
doaj   +2 more sources

Development of a novel nanobody-based competitive ELISA for rapid detection of antibodies against Senecavirus A [PDF]

BMC Veterinary Research
Background Senecavirus A (SVA) causes considerable economic losses in the swine industry, particularly among piglets. However, effective serological diagnostic assays for SVA are still lacking. A testing platform based on nanobody-horseradish peroxidase (
Longzhi Nie, Tianxiang Chen, Yunpeng Shi, Bing Wu, Chao Xiong, Qin Zhao, Yuchen Nan, Yiyang Chen, Baoyuan Liu   +8 more
doaj   +2 more sources

A McAb-Based Direct Competitive ELISA to Detect O:9 Salmonella Infection in Chicken [PDF]

Frontiers in Veterinary Science, 2020
Salmonella enteritidis and Salmonella pullorum belonging to Group O9Salmonella are major causative agents of infectious diseases in chicken. O9 antigen as a part of lipopolysaccharide (LPS) is a predominant detected target for Salmonella infection.
Zemiao Xia, Zemiao Xia, Zemiao Xia, Zemiao Xia, Haopeng Geng, Haopeng Geng, Haopeng Geng, Haopeng Geng, Yuan Cai, Yuan Cai, Yuan Cai, Yuan Cai, Yaonan Wang, Yaonan Wang, Yaonan Wang, Yaonan Wang, Daquan Sun, Daquan Sun, Daquan Sun, Daquan Sun, Jian Zhang, Jian Zhang, Jian Zhang, Jian Zhang, Zhiming Pan, Zhiming Pan, Zhiming Pan, Zhiming Pan, Xin'an Jiao, Xin'an Jiao, Xin'an Jiao, Xin'an Jiao, Shizhong Geng, Shizhong Geng, Shizhong Geng, Shizhong Geng   +35 more
doaj   +2 more sources

Preparation of a house ELISA kit for detecting Peste des petits ruminants Virus (PPRV) antibodies [PDF]

Benha Veterinary Medical Journal, 2021
Peste des petits ruminants (PPR) is a viral disease that affects small ruminants characterized by fever, highly contagious, and economically significant. For diagnosis of the disease, effective diagnostic techniques are essential.
Wafaa Hosny, Eman Baheeg, Sozan Hassanein, Sahar Mohamed   +3 more
doaj   +1 more source

Current situation of Peste des petits ruminants (PPR) virus disease in some Egyptian Governorates in the period between years 2014 to 2019 [PDF]

Egyptian Journal of Animal Health, 2021
Peste des petits ruminants (PPR) is a highly contagious disease of domestic and wild small ruminants. According to Office international des epizooties  (OIE), it is now recognized as a disease of great economic importance, causing huge annual losses in ...
doaj   +1 more source

Development of Sandwich and Competitive ELISA Formats to Determine Soybean Allergen: Evaluation of Their Performance to Detect Soy in Processed Food

Shipin gongye ke-ji, 2022
Taking soybean mixed allergens as the target, a sandwich-enzyme linked immunosorbent assay and an indirect competitive enzyme-linked immunosorbent assay for the rapid and convenient detection of soybean allergens were established.
Liyan ZHU, Siyue LI, Jianlian HUANG, Yinmei JIANG, Shuo WAN, Jinlong ZHAO, Zhenxing LI   +6 more
doaj   +1 more source

Influenza Anti-Stalk Antibodies: Development of a New Method for the Evaluation of the Immune Responses to Universal Vaccine

Vaccines, 2020
Growing interest in universal influenza vaccines and novel administration routes has led to the development of alternative serological assays that are able to detect antibodies against conserved epitopes.
Alessandro Manenti, Agnieszka Katarzyna Maciola, Claudia Maria Trombetta, Otfried Kistner, Elisa Casa, Inesa Hyseni, Ilaria Razzano, Alessandro Torelli, Emanuele Montomoli   +8 more
doaj   +1 more source

Gluten-Free Foods Cooked in Shared Fryers With Wheat: A Pilot Study Assessing Gluten Cross Contact

Frontiers in Nutrition, 2021
Introduction: Consumers with celiac disease are discouraged from eating fried foods cooked in shared fryers with wheat-containing foods at restaurants based on presumed gluten exposure. The purpose of the present study is to assess gluten levels of fries
Tricia Thompson, Trisha Bury Lyons, Amy Keller, Nancee Jaffe, Luke Emerson-Mason   +4 more
doaj   +1 more source

Development of indirect competitive ELISA for determination of dehydroabietic acid in duck skin and comparison with the HPLC method

Poultry Science, 2020
Defeathering with rosin results in rosin residue in duck skin, which may present as potential risk to human health. Dehydroabietic acid (DHAA) is a major component of rosin.
Xinyuan Qiu, Jingjing Ma, Pengpeng Li, Zhiming Geng, Chong Sun, Daoying Wang, Weimin Xu   +6 more
doaj   +1 more source

Competitive ELISA for a serologic test to detect dengue serotype-specific anti-NS1 IgGs using high-affinity UB-DNA aptamers

Scientific Reports, 2021
Serologic tests to detect specific IgGs to antigens related to viral infections are urgently needed for diagnostics and therapeutics. We present a diagnostic method for serotype-specific IgG identification of dengue infection by a competitive enzyme ...
Ken-ichiro Matsunaga, Michiko Kimoto, Vanessa Weixun Lim, Tun-Linn Thein, Shawn Vasoo, Yee-Sin Leo, William Sun, Ichiro Hirao   +7 more
doaj   +1 more source