Development of Indirect Competitive ELISA and Visualized Multicolor ELISA Based on Gold Nanorods Growth for the Determination of Zearalenone [PDF]
Foods, 2021 In this study, a zearalenone (ZEN) hapten was designed and prepared against the mycotoxin ZEN, and the original coating ZEN-ovalbumin (ZEN-OVA) was prepared by conjugation with OVA. Based on the gold nanorods (AuNRs) of uniform size and stable properties
Tianyu Ma +5 more
doaj +7 more sources
Electrochemical Immunosensor for Detection of Aflatoxin B1 Based on Indirect Competitive ELISA [PDF]
Toxins, 2018 Mycotoxins are the secondary toxic metabolites produced naturally by fungi. Analysis of mycotoxins is essential to minimize the consumption of contaminated food and feed.
Farah Asilah Azri +5 more
doaj +7 more sources
An indirect competitive ELISA for determination of guanidine acetic acid in animal feed [PDF]
Scientific ReportsTo establish an indirect competitive ELISA (ci-ELISA) for the detection of guanidino acetic acid (GAA) residues in animal feed, in this study, GAA was coupled to carrier proteins via the active ester method to obtain an anti-GAA complete antigen (GAA-BSA)
Xingdong Yang +7 more
doaj +4 more sources
A Monoclonal Antibody-Based Indirect Competitive ELISA for Detecting Goose Astrovirus Antibodies [PDF]
Veterinary SciencesGoose astrovirus (GoAstV) infection has become prevalent in major goose-producing regions, causing substantial economic losses to the industry. In this study, an indirect competitive ELISA (ic-ELISA) was developed based on a monoclonal antibody (mAb ...
Junfeng Lv +6 more
doaj +4 more sources
A pan-genotypic indirect competitive ELISA for serological detection of pigeon circovirus antibodies [PDF]
Frontiers in MicrobiologyPigeon circovirus (PiCV) infection, which causes young pigeon disease syndrome (YPDS) and immunosuppression, significantly impacts both the meat and racing pigeon industries.
Weifan Wang +16 more
doaj +5 more sources
Development of an indirect competitive ELISA for simultaneous detection of enrofloxacin and ciprofloxacin [PDF]
Journal of Zhejiang University: Science B, 2011 Modified 1-ethyl-3-(3-dimethylaminopropy) carbodiimide (EDC) method was employed to synthesize the artificial antigen of enrofloxacin (ENR), and New Zealand rabbits were used to produce anti-ENR polyclonal antibody (pAb). Based on the checkerboard titration, an indirect competitive enzyme-linked immunosorbent assay (ELISA) standard curve was ...
Zhang HT +7 more
exaly +5 more sources
Nanobody-based indirect competitive ELISA for the detection of aflatoxin M1 in dairy products [PDF]
Scientific ReportsAflatoxin M1 (AFM1) is known to be carcinogenic, mutagenic, and teratogenic and poses a serious threat to food safety and human health, which makes its surveillance critical.
Li Yi +6 more
doaj +4 more sources
Nanobody-Based Indirect Competitive ELISA for Sensitive Detection of 19-Nortestosterone in Animal Urine [PDF]
Biomolecules, 2021 Nanobody (Nb), a new type of biorecognition element generally from Camelidae, has the characteristics of small molecular weight, high stability, great solubility and high expression level in E. coli.
Yuan-yuan Yang +8 more
doaj +2 more sources
Development of indirect competitive ELISA for determination of dehydroabietic acid in duck skin and comparison with the HPLC method [PDF]
Poultry Science, 2020 Defeathering with rosin results in rosin residue in duck skin, which may present as potential risk to human health. Dehydroabietic acid (DHAA) is a major component of rosin.
Xinyuan Qiu +6 more
doaj +2 more sources
Establishment of an Indirect Competitive ELISA for Zearalenone
Shipin gongye ke-ji, 2022 In this study, an indirect competitive ELISA (ic-ELISA) was developed for the detection of zearalenone in corn flour. The optimal working concentration of antigen and monoclonal antibody was determined by chessboard method.
Song TANG +7 more
doaj +2 more sources