Development of an Indirect Competitive ELISA Based on a Stable Epitope of β-Lactoglobulin for Its Detection in Hydrolyzed Formula Milk Powder [PDF]
FoodsThe target of traditional immunological detection methods for milk allergens is usually the whole β-lactoglobulin molecule. However, thermal processes and hydrolysis can destroy the epitope of β-lactoglobulin and interfere with its accurate detection and
Qinggang Xie +4 more
doaj +3 more sources
Determination of Ofloxacin Residues in Honey by Indirect Competition ELISA [PDF]
Shipin gongye ke-jiIn order to establish an indirect competitive ELISA for the detection of ofloxacin (OFLX) residues in honey, in this study, OFLX was coupled to carrier proteins using the active ester method to obtain anti-OFLX complete antigen (OFLX-BSA) and detection ...
Xingdong YANG, Huiya WEI, Yang QU
doaj +3 more sources
Indirect Competitive ELISA for the Determination of Total Chromium Content in Food, Feed and Environmental Samples [PDF]
Molecules, 2022 Background: This study aimed to prepare monoclonal antibodies (mAbs) with high immunoreactivity, sensitivity, and specificity for the chelate (Cr(III)-EDTA) of trivalent chromium ion (Cr(III)) and ethylenediamine tetraacetic acid (EDTA).
Xiaofei Wang +5 more
doaj +2 more sources
Development of IgY-Based Indirect Competitive ELISA for the Detection of Fluoroquinolone Residues in Chicken and Pork Samples [PDF]
Antibiotics, 2022 Fluoroquinolones (FQs) are among the antibiotics whose widespread use in farm-raised animals results in potentially harmful residues in the end products. Additionally, most Thai farmers use antibiotics.
Sumed Yadoung +8 more
doaj +2 more sources
Development of Indirect Competitive ELISA and Colloidal Gold Immunochromatographic Strip for Endosulfan Detection Based on a Monoclonal Antibody [PDF]
Foods, 2023 Endosulfan, as an effective broad-spectrum insecticide, has been banned in agricultural areas because of the potential harmful effects on human health. This study aimed to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and ...
Xinghua Zhou +5 more
doaj +2 more sources
Establishment of Indirect Competitive ELISA Method for Detecting Amanitin in Mushroom
Shipin gongye ke-ji, 2022 Objective: This study aims to establish an indirect competitive enzyme-linked immunosorbent assay for the detection of amanitin (AMA) in mushroom. Methods: In this study, the hapten was obtained by introducing 6-aminocaproic acid into the carboxyl ...
Hebing LIU +3 more
doaj +2 more sources
Establishment of an indirect competitive ELISA for sulfadimethoxine
Shipin yu jixieObjectiveTo establish an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for the detection of sulfadimethoxine (SDM) in animal-derived foods.MethodsAn artificial antigen is synthesized by conjugating SDM with carrier proteins by the ...
Han Lu +6 more
doaj +2 more sources
Hapten Synthesis and the Development of an Ultrasensitive Indirect Competitive ELISA for the Determination of Diethylstilbestrol in Food Samples [PDF]
Scientific Reports, 2020 An ultrasensitive indirect competitive enzyme-linked immunosorbent assay (ic ELISA) using monoclonal antibodies (mAbs) was developed for the specific detection of diethylstilbestrol (DES) residues.
Xingdong Yang +5 more
doaj +2 more sources
Pharmacokinetics and Tissue Distribution Kinetics of Puerarin in Rats Using Indirect Competitive ELISA. [PDF]
Molecules, 2017 Puerarin (PUE) is a compound isolated from the roots of Pueraria lobata. We studied the pharmacokinetics and tissue distribution kinetics of PUE in Sprague-Dawley rats following intraperitoneal administration of three concentrations. Indirect competitive ELISA based on an anti-PUE monoclonal antibody was used to determine the concentration of PUE in ...
Kong H +10 more
europepmc +4 more sources
Development of Indirect Competitive ELISA for Lithospermic Acid B of Salvia miltiorrhiza with Its Specific Antibodies Generated via Artificial Oil Bodies [PDF]
Molecules, 2019 Lithospermic acid B (LSB), the major water-soluble ingredient of Salvia miltiorrhiza (Danshen), has been shown to be an active ingredient responsible for the therapeutic effects of this traditional Chinese herb used to treat cardiac disorders. This study
Yu-En Shih +3 more
doaj +2 more sources