Results 61 to 70 of about 2,073 (157)

Immunoassay Detection of SARS‐CoV‐2 Using Monoclonal Antibody Binding to Viral Nucleocapsid Protein

Microbial Biotechnology, Volume 18, Issue 2, February 2025.
Selective detection of SARS‐CoV viral variants by immunoassay with monoclonal antibodies (MAbs) 4D4 and 1H6 that bind nucleocapsid protein (NP). Sensitive detection of SARS‐CoV‐2 NP and SARS‐CoV‐2 virus variants by sandwich ELISA (left) and lateral flow immunoassay (right).
Robert M. Hnasko, Alice V. Lin, Jeffery A. McGarvey, Eric S. Jackson   +3 more
wiley   +1 more source

Beyond Serology: A Meta‐Analysis of Advancements in Molecular Detection of Brucella spp. in Seronegative Animals and Biological Samples

Veterinary Medicine and Science, Volume 11, Issue 1, January 2025.
Molecular diagnostics, such as PCR and biosensor‐based techniques, offer high sensitivity in detecting Brucella spp. in seronegative animals, overcoming the limitations of traditional serological methods. The pooled detection rate of 35.08% suggests a substantial number of hidden carriers.
Md. Sadequl Islam, Md. Ahsan Habib, Nasrin Sultana Tonu, Md. Samiul Haque, Md. Mostafizer Rahman   +4 more
wiley   +1 more source

Progress Toward Antigenic Epitopes of African Swine Fever Virus and Their Identification

Transboundary and Emerging Diseases, Volume 2025, Issue 1, 2025.
African swine fever virus (ASFV) is a highly contagious pathogen that causes pigs to develop high fever, vomiting, diarrhea, and respiratory distress, with an exceptionally high case fatality rate. Unfortunately, vaccine development is hindered by a limited understanding of the structure and function of the protein encoded by ASFV, as well as the ...
Xingjun Ke, Zhi Cao, Zhen Weng, Yin Xie, Fengyu Wu, Xinzhu Liu, Shiying Zhou, Mengjie Lian, Tongyu Liu, Ruize Sun, Lerong Ma, Aishi Xu, Jiaqi Wang, Hongsheng Ouyang, Linzhu Ren, Daxin Pang, Dongmei Lv, Nan-hua Chen   +17 more
wiley   +1 more source

High molecular mass fraction in clinical isolates of Paracoccidioides brasiliensis Fração de alta massa molecular em isolados clínicos de Paracoccidioides brasiliensis

Revista da Sociedade Brasileira de Medicina Tropical, 2010
INTRODUCTION: Different serum levels of the IgG/IgE for Paracoccidioides brasiliensis high mass molecular (hMM) fraction (~366kDa) in the acute and chronic forms of the disease have been reported.
Andréa Longoni Fredrich, Luciene Airy Nagashima, Wander Rogério Pavanelli, Audrey de Souza Marquez, Mari Sumigawa Kaminami, Nilson de Jesus Carlos, Ayako Sano, Mario Augusto Ono, Eiko Nakagawa Itano   +8 more
doaj   +1 more source

National Seroprevalence and Risk Factors of Bluetongue Virus in Domestic Ruminants of Peru

Transboundary and Emerging Diseases, Volume 2025, Issue 1, 2025.
Bluetongue (BT) is a viral infection caused by the bluetongue virus (BTV) that affects domestic and wild ruminants worldwide. It is primarily transmitted by Culicoides spp. midges, and its infection is highly prevalent across temperate and tropical regions.
Dennis A. Navarro-Mamani, Jessica Jurado, Ana Vargas-Calla, Kevin Ponce, Tyler Sherman, Yari Zarate, César A. Murga-Moreno, Ibelice Perez, Ruben Villacaqui, Miguel Ara, Pedro Ortiz, Hermelinda Rivera, Christie E. Mayo, Fedor Korennoy   +13 more
wiley   +1 more source

Prevalence of equine Piroplasmosis and its association with tick infestation in the State of São Paulo, Brazil Prevalência da Piroplasmose equina e sua associação com infestação por carrapatos no Estado de São Paulo

Revista Brasileira de Parasitologia Veterinária, 2009
Serum samples were collected from 582 horses from 40 stud farms in the State of São Paulo and tick (Acari: Ixodidae) infestations were evaluated on them.
Claudia E. Kerber, Marcelo B. Labruna, Fernando Ferreira, Daniel T. De Waal, Donald P. Knowles, Solange M. Gennari   +5 more
doaj   +1 more source

Study of the presence of specific Salmonella Enteritidis antibodies in chicken egg yolks by competitive cELISA method

Acta veterinaria, 2011
One of the most common causes of salmonellosis of man and poultry is Salmonella Enteritidis which is often found in the digestive system of adult birds. The infected birds do not display any evident clinical symptoms and, at the same time, they excrete the bacteria into the surrounding environment.
Radojičić, Marina, Milić, Nenad, Nišavić, Jakov, Marković, Maja   +3 more
openaire   +3 more sources

Prevalence of equine infectious anemia virus in horses and donkeys determined by comparison of ELISA and AGID in Mexico

Arquivo Brasileiro de Medicina Veterinária e Zootecnia
From February to December 2021, 960 serum samples from different climate regions in two Mexican states were analyzed for antibodies against EIAV using competitive enzyme-linked immunosorbent assay (cELISA) and agar gel immunodiffusion (AGID) tests.
A. Villa-Mancera, L. Villegas-Bello, H. Campos-García, S. Ortega-Vargas, J. Cruz-Aviña, F. Patricio-Martínez, J. Olivares-Pérez, F. Utrera-Quintana   +7 more
doaj   +1 more source

Preparation of a monoclonal antibody against recombinant LSDV034 protein and its application in detecting lumpy skin disease virus through a competitive enzyme-linked immunosorbent assay (cELISA)

Animal Diseases
Lumpy skin disease (LSD) is a highly contagious disease caused by lumpy skin disease virus (LSDV) in bovines. Rapid and accurate diagnosis is very important to controll it.
Xinwei Yuan, Hui Zhao, Wanfeng Ji, Xiaohan Yan, Zhijie Xiang, Li Yang, Yuanchen Geng, Yingyu Chen, Jianguo Chen, Xi Chen, Lei Zhang, Changmin Hu, Aizhen Guo   +12 more
doaj   +1 more source

Production of Polyclonal Antibodies and Development of Competitive ELISA for Quantification of the Lantibiotic Paenibacillin

Fermentation
The discovery and biotechnological application of new antimicrobial peptides are impeded by a lack of sensitive methods for peptide quantification. Paenibacillin is an emerging antimicrobial lantibiotic that was discovered in Paenibacillus polymyxa OSY ...
Ahmed G. Abdelhamid, Macdonald Wick, Ahmed E. Yousef   +2 more
doaj   +1 more source