Results 211 to 220 of about 1,647,355 (316)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Plasma Cell-free DNA Concentration and Outcomes from Taxane Therapy in Metastatic Castration-resistant Prostate Cancer from Two Phase III Trials (FIRSTANA and PROSELICA)

, 2018
Niven Mehra, David Dolling, Semini Sumanasuriya, Rossitza Christova, Lorna Pope, Suzanne Carreira, George Seed, Wei Yuan, Jane Goodall, Emma Hall, Penny Flohr, Gunther Boysen, Diletta Bianchini, Oliver Sartor, Mario A. Eisenberger, Karim Fizazi, Stéphane Oudard, Mustapha Chadjaa, Sandrine Macé, Johann S. de Bono   +19 more
openalex   +1 more source

Circulating Cell-Free Unmethylated DNA as a Marker of Graft Dysfunction in Pancreas Transplantation [PDF]

, 2016
Iestyn M. Shapey, Angela Summers, Titus Augustine, Martin K. Rutter, David van Dellen   +4 more
openalex   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Cell-Free DNA-Derived Immune Cell Ratios Uncover Cancer-Associated Systemic Changes. [PDF]

Cancer Res Commun
Andersen L, Lindskrog SV, Nordentoft I, Frydendahl A, Nors J, Henriksen TV, Rasmussen MH, Iversen LH, Gotschalck KA, Jensen JB, Dyrskjøt L, Andersen CL, Birkbak NJ.   +12 more
europepmc   +1 more source

Supplementary Figure S3 from Cell-free DNA Concentration as a Biomarker of Response and Recurrence in HER2-Negative Breast Cancer Receiving Neoadjuvant Chemotherapy

Mark Jesus M. Magbanua, Ziad Ahmed, Rosalyn W. Sayaman, Lamorna Brown Swigart, Gillian L. Hirst, Christina Yau, Denise M. Wolf, Wen Li, Amy L. Delson, Jane Perlmutter, Paula R. Pohlmann, W. Fraser Symmans, Douglas Yee, Nola M. Hylton, Laura J. Esserman, Angela DeMichele, Hope S. Rugo, Laura van ‘t Veer   +17 more
openalex   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Multimodal analysis of cell-free DNA identifies epigenetic biomarkers for amyotrophic lateral sclerosis diagnosis and progression. [PDF]

J Clin Invest
Michels S, Chen C, Ruf WP, Garcia Garcia MM, Arnold FJ, Wu Z, Bennett CL, Shams D, Thompson LM, Walker AC, Dickson DW, Petrucelli L, Dorst J, Prudencio M, Li W, La Spada AR.   +15 more
europepmc   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

cfGWAS reveal genetic basis of cell-free DNA end motifs. [PDF]

Nat Commun
Zhu H, Zhang Y, Li L, Zeng S, Zhang X, Lin Y, Ou R, Wang L, Li X, Wang Y, Zeng J, Lin Y, Xu C, Zeng G, Li L, Zhao R, Jia Y, Luo F, Yang M, Hu Y, Xiao H, Xu X, Wang J, Zhou A, Zhang H, Jin X.   +25 more
europepmc   +1 more source