Results 101 to 110 of about 1,959,228 (334)

Protocol for real-time measurement of mitochondrial bioenergetics in 3D-cultured brain tumor stem cells using the Resipher system

STAR Protocols
Summary: Aberrant mitochondrial function can lead to severe human diseases, including neurodegenerative diseases and cancer. Here, we describe a cell-based protocol for measuring different mitochondrial respiratory parameters using the high-resolution ...
Cloé Tessier, Maxime Toujas, Antonio C. Pagano Zottola, Andreas Bikfalvi, Thomas Mathivet, Thomas Daubon, Lucie Brisson, Audrey Burban, Ahmad Sharanek   +8 more
doaj   +1 more source

The role of fibroblast growth factors in cell and cancer metabolism

FEBS Letters, EarlyView.
Fibroblast growth factor (FGF) signaling regulates crucial signaling cascades that promote cell proliferation, survival, and metabolism. Therefore, FGFs and their receptors are often dysregulated in human diseases, including cancer, to sustain proliferation and rewire metabolism.
Jessica Price, Chiara Francavilla
wiley   +1 more source

Protocol for genetic dissection of class switch recombination using genome-editing tools

STAR Protocols
Summary: Antibody class switch recombination is achieved through programmed DNA damage, and the processing of programmed DNA lesions requires the coordinated action of many DNA metabolic factors.
Pengfei Dai, Tingting Liu, Dingpeng Yang, Yifeng Luo, Liu Daisy Liu, Xia Xie, Yafang Shang, Xiaojing Liu, Fei-Long Meng   +8 more
doaj   +1 more source

Establishment of live-cell-based coupled assay system for identification of compounds to modulate metabolic activities of cells [PDF]

, 2021
Kouichi Yanagi, Toru Komatsu, Shusuke Ogihara, Takayoshi Okabe, Hirotatsu Kojima, Tetsuo Nagano, Tasuku Ueno, Kenjiro Hanaoka, Yasuteru Urano   +8 more
openalex   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Protocol to assess bioenergetics and mitochondrial fuel usage in murine autoreactive immunocytes using the Seahorse Extracellular Flux Analyzer

STAR Protocols
Summary: Efficient metabolism, or the means by which cells produce energy resources, is critical for proper effector function. Here, we present a protocol for examining the bioenergetics and mitochondrial fuel utilization of primary murine autoreactive ...
Colleen L. Mayberry, John J. Wilson, Britney Sison, Chih-Hao Chang   +3 more
doaj   +1 more source

Comparison of intracellular cytokine staining versus an ELISA‐based assay to assess CMV‐specific cell‐mediated immunity in high‐risk kidney transplant recipients [PDF]

, 2023
Mario Fernández‐Ruiz, Natalia Redondo, Patricia Parra, Tamara Ruiz‐Merlo, Isabel Rodríguez‐Góncer, Natalia Polanco, Marta Nicolini Falcão do Monte, Francisco López‐Medrano, Rafael San Juan, David Navarro, Amado Andrés, José María Aguado   +11 more
openalex   +1 more source

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

The role of initial geometry in experimental models of wound closing

, 2017
Wound healing assays are commonly used to study how populations of cells, initialised on a two-dimensional surface, act to close an artificial wound space.
Chou, Shih-En, Jin, Wang, Lo, Kai-Yin, McCue, Scott W, Simpson, Matthew J   +4 more
core   +1 more source

The IQ‐compete assay for measuring mitochondrial protein import efficiencies in living yeast cells

FEBS Letters, EarlyView.
The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Yasmin Hoffman, Annika Egeler, Saskia Rödl, Johannes M. Herrmann   +3 more
wiley   +1 more source