Results 211 to 220 of about 1,374,920 (267)

Microchips for Cell-Based Assays

2009
Analysis of cells is a cornerstone in biomedical research. Traditional methods for cell culture and tissue analysis can be replaced by various microchips as discussed in this chapter. A tissue array is an example of microchip that provides higher throughput of tissue analysis.
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Cell based assays

2017
Tumor necrosis factor alpha (TNFa) not only plays a key role in the human immune system, but also has a cytotoxic effect on tumor cells. Owing to this function, TNFa can become a considerable candidate for chemotherapy but needs to be delivered locally.
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Cell-Based Assays on Microfluidics for Drug Screening

ACS Sensors, 2019
Microfluidics is an appealing platform for drug screening and discovery. Compared with the conventional drug screening methods based on Petri dishes and experimental animals, microfluidic devices have many advantages including miniaturized size, ease-to-use, high sensitivity, and high throughput.
Xiaoyan Liu, Wenfu Zheng, Xingyu Jiang
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The Problems with the Cells Based Assays

Cell Biology: Research & Therapy, 2012
The Problems with the Cells Based Assays An important part of the classical drug discovery paradigm is the cell based assay. Medicinal chemists, after identification of the molecular target such as enzyme or receptor are quite efficient with the design and synthesis of a potential inhibitor or receptor antagonist.
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Cell-based assays for kinase drug discovery

Drug Discovery Today: Technologies, 2010
Kinases are important therapeutic targets. Potent in vitro inhibitors are readily produced, but these compounds are not always potent in cells. Because cell potency is crucial for efficacy, cell-based assays are important for successful inhibitor development. In this review kinase cell assays are divided into four types.
Gary K. Smith, Edgar R. Wood
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Cell-Based Assays for High-Throughput Screening

Molecular Biotechnology, 2010
Cell-based assays represent approximately half of all high-throughput screens currently performed. Here, we review in brief the history and status of high-throughput screening (HTS), and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS.
W Frank, An, Nicola, Tolliday
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Cell-based assay for β-secretase activity

Analytical Biochemistry, 2003
The cerebral deposition of amyloid beta-peptide (Abeta) is a major factor in the etiology of Alzheimer's disease. beta-Secretase (BACE) initiates the generation of Abeta by cleaving the amyloid precursor protein at the beta-site and is therefore a prime target for therapeutic intervention. Here we report a cell-based method suitable for monitoring BACE
Myungsok, Oh, Sung Yun, Kim, Yeong Soo, Oh, Deog-Young, Choi, Hye Jin, Sin, Inhee Mook, Jung, Woo Jin, Park   +6 more
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Cell-Based Assays for Regulators of Tau Biology

2010
Understanding the molecular mechanisms of Alzheimer's disease (AD) is a challenging endeavor, namely, due to the fact that the disease only occurs in the central nervous system of elderly humans. Thus, model systems simply do not accurately portray this cellular landscape. While we cannot ask many mechanistic questions using the human brain as our test
Umesh K, Jinwal, Chad A, Dickey
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High-throughput cell-based assays in yeast

Drug Discovery Today, 2002
With the sequencing of many genomes now completed, biologists are faced with the challenge of deciphering the function and association of an immense number of predicted proteins. Comprehension of the networks of proteins and chemicals that comprise the cells and tissues of an organism, and the specific roles of proteins in these networks, will be a ...
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Label-Free Cell-Based Functional Assays

Combinatorial Chemistry & High Throughput Screening, 2008
Label-free technologies based on electrical impedance or refractive index are new tools for measuring a cell-based functional response. Although the technologies are relatively new to high throughput screening cell-based applications, they are rapidly generating interest in that they are able to measure a phenotypic response using cells natively ...
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