Results 41 to 50 of about 8,914,547 (304)

The Role of Hematopoietic Cell Transplantation in Ataxia‐Telangiectasia

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Ataxia‐telangiectasia (A‐T) is a DNA repair disorder characterized by neurodegeneration, immunodeficiency, and cancer predisposition. Hematopoietic cell transplantation (HCT) is an established therapy in related disorders such as Fanconi anemia (FA) and Nijmegen breakage syndrome (NBS), but its role in A‐T is unclear.
Laila Alkhouli, Seth Rotz, Masatoshi Takagi, Richa Sharma   +3 more
wiley   +1 more source

GATS tag system is compatible with biotin labelling methods for protein analysis

Scientific Reports, 2023
Polypeptide tags and biotin labelling technologies are widely used for protein analyses in biochemistry and cell biology. However, many peptide tag epitopes contain lysine residues (or amino acids) that are masked after biotinylation.
Kohdai Yamada, Fumiya Soga, Soh Tokunaga, Hikaru Nagaoka, Tatsuhiko Ozawa, Hiroyuki Kishi, Eizo Takashima, Tatsuya Sawasaki   +7 more
doaj   +1 more source

Efficacy and Safety Analysis of Roxarestat in Regulating Renal Anemia in Patients on Maintenance Hemodialysis

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Objective To compare the efficacy and safety of roxarestat versus recombinant human erythropoietin (rhEPO) in the management of renal anemia in patients undergoing maintenance hemodialysis. Methods This was a prospective, open‐label, randomized controlled trial.
Lingling Chen, Junjie Zhu, Qiaonan Ge
wiley   +1 more source

Streamlined extract preparation for Escherichia coli-based cell-free protein synthesis by sonication or bead vortex mixing

BioTechniques, 2012
Escherichia coli-based cell extract is a vital component of inexpensive and high-yielding cell-free protein synthesis reactions. However, effective preparation of E.
Prashanta Shrestha, Troy Michael Holland, Bradley Charles Bundy   +2 more
doaj   +1 more source

Systematic review of extracellular vesicle-based treatments for lung injury: are EVs a potential therapy for COVID-19?

Journal of Extracellular Vesicles, 2020
Severe COVID-19 infection results in bilateral interstitial pneumonia, often leading to acute respiratory distress syndrome (ARDS) and pulmonary fibrosis in survivors. Most patients with severe COVID-19 infections who died had developed ARDS.
Kasra Khalaj, Rebeca Lopes Figueira, Lina Antounians, Giuseppe Lauriti, Augusto Zani   +4 more
doaj   +1 more source

Characterizing and Improving pET Vectors for Cell-free Expression

Frontiers in Bioengineering and Biotechnology, 2022
Cell-free protein synthesis (CFPS) is an in vitro process that enables diverse applications in research, biomanufacturing, point-of-care diagnostics, therapeutics, and education using minimal laboratory equipment and reagents.
Kara Jew, Philip E. J. Smith, Byungcheol So, Jillian Kasman, Javin P. Oza, Michael W. Black   +5 more
doaj   +1 more source

Effects of the Fluid Replacement Method During Online Hemodiafiltration on the Solute Removal Performance and Biocompatibility Using the Asymmetric Cellulose Triacetate Membrane

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Introduction Pre‐dilution online hemodiafiltration (Pre‐HDF) is predominantly used in Japan, whereas post‐dilution online HDF (Post‐HDF) is more common in Europe. An asymmetric cellulose triacetate (ATA) membrane may improve biocompatibility.
Kenji Sakurai, Yoshitaka Kurihara, Fumi Yamauchi, Hiromi Hosoya, Takeshi Saito   +4 more
wiley   +1 more source

Effectiveness of a Novel Low‐Density Lipoprotein Apheresis Device Rheocarna in Patients Undergoing Hemodialysis With Chronic Limb‐Threatening Ischemia: A Single‐Center Retrospective Study

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Background Chronic limb‐threatening ischemia (CLTI) is a severe form of lower‐extremity artery disease characterized by distal lesions and microcirculatory impairment, limiting revascularization efficacy. Rheocarna is a direct hemoperfusion low‐density lipoprotein (LDL) adsorption device with potential rheological and anti‐inflammatory ...
Kunihiro Ishioka, Takayasu Ohtake, Kei Ohmori, Chika Shibuya, Tomoki Tsukahara, Mizuki Yamano, Yasuhiro Mochida, Machiko Oka, Kyoko Maesato, Yusuke Tsukamoto, Hidekazu Moriya, Sumi Hidaka, Shuzo Kobayashi   +12 more
wiley   +1 more source

In vitro extracellular replication of Wolbachia endobacteria

Frontiers in Microbiology
Obligate intracellular endobacteria of the genus Wolbachia are widespread in arthropods and several filarial nematodes. Control programs for vector-borne diseases (dengue, Zika, malaria) and anti-filarial therapy with antibiotics are based on this ...
Lara Vanessa Behrmann, Kirstin Meier, Jennifer Vollmer, Chukwuebuka Chibuzo Chiedu, Andrea Schiefer, Achim Hoerauf, Achim Hoerauf, Kenneth Pfarr, Kenneth Pfarr   +8 more
doaj   +1 more source

Cell-Free Expression to Probe Co-Translational Insertion of an Alpha Helical Membrane Protein

Frontiers in Molecular Biosciences, 2022
The majority of alpha helical membrane proteins fold co-translationally during their synthesis on the ribosome. In contrast, most mechanistic folding studies address refolding of full-length proteins from artificially induced denatured states that are ...
Laura R. Blackholly, Nicola J. Harris, Heather E. Findlay, Paula J. Booth   +3 more
doaj   +1 more source