Results 71 to 80 of about 2,936,501 (309)

Do-it-yourself instrument integration into an existing mammalian cell line development automation platform

SLAS Technology
Laboratory automation in the biopharmaceutical industry as a rule requires contracted service from highly professional automation solution provider, at times involving the purchase and development of specialized or customized hardware and software, which
Jie Ding, Kee Wee Tan, Xiaoyue Chen
doaj   +1 more source

Evaluation of pre-analytical factors affecting plasma DNA analysis. [PDF]

, 2018
Pre-analytical factors can significantly affect circulating cell-free DNA (cfDNA) analysis. However, there are few robust methods to rapidly assess sample quality and the impact of pre-analytical processing. To address this gap and to evaluate effects of
Berens, Michael E, Borad, Mitesh J, Bryce, Alan, Contente-Cuomo, Tania, Dhruv, Harshil D, Farooq, Maria, Gollins, Simon, Liang, Winnie S, LoRusso, Patricia M, Markus, Havell, Murtaza, Muhammed, Ribas, Antoni, Sekulic, Aleksandar, Sivakumar, Shivan, Tran, Nhan L, Trent, Jeffrey M   +15 more
core   +1 more source

Evaluating the Utility of Paired Tumor and Germline Targeted DNA Sequencing for Pediatric Oncology Patients: A Single Institution Report

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Objective To evaluate the diagnostic yield and utility of universal paired tumor–normal multigene panel sequencing in newly diagnosed pediatric solid and central nervous system (CNS) tumor patients and to compare the detection of germline pathogenic/likely pathogenic variants (PV/LPVs) against established clinical referral criteria for cancer ...
Natalie Waligorski, Rebecca Ronsley, Shannon M. Stasi, Jeffrey Stevens, Erin R. Rudzinski, Christina M. Lockwood, Sarah E. S. Leary, Bonnie L. Cole, Michelle A. Ting, Vera Paulson   +9 more
wiley   +1 more source

Cell-Free Expression to Probe Co-Translational Insertion of an Alpha Helical Membrane Protein

Frontiers in Molecular Biosciences, 2022
The majority of alpha helical membrane proteins fold co-translationally during their synthesis on the ribosome. In contrast, most mechanistic folding studies address refolding of full-length proteins from artificially induced denatured states that are ...
Laura R. Blackholly, Nicola J. Harris, Heather E. Findlay, Paula J. Booth   +3 more
doaj   +1 more source

Cell-Free Synthesis Meets Antibody Production: A Review

Antibodies, 2015
Engineered antibodies are key players in therapy, diagnostics and research. In addition to full size immunoglobulin gamma (IgG) molecules, smaller formats of recombinant antibodies, such as single-chain variable fragments (scFv) and antigen binding ...
Marlitt Stech, Stefan Kubick
doaj   +1 more source

In situ Adipogenesis in Biomaterials Without Cell Seeds: Current Status and Perspectives

Frontiers in Cell and Developmental Biology, 2021
For cosmetic and reconstructive purposes in the setting of small-volume adipose tissue damage due to aging, traumatic defects, oncological resections, and degenerative diseases, the current strategies for soft tissue replacement involve autologous fat ...
Jiqiao Yang, Jiqiao Yang, Chen Zhou, Jingyang Fu, Qianru Yang, Tao He, Qiuwen Tan, Qiuwen Tan, Qing Lv   +8 more
doaj   +1 more source

Performance of screening for aneuploidies by cell-free DNA analysis of maternal blood in twin pregnancies [PDF]

, 2015
Objectives To report clinical implementation of cell‐free DNA (cfDNA) analysis of maternal blood in screening for trisomies 21, 18 and 13 in twin pregnancies and examine variables that could influence the failure rate of the test.
BEVILACQUA, ELISA, E. Ordoñez, H. Dierickx, JC Jani, KH Nicolaides, MM Gil, PJ Willems, V. Cirigliano   +7 more
core   +1 more source

Preferences of Pediatric Patients and Their Caregivers for Chemotherapy‐Induced Nausea and Vomiting Control Endpoints: A Mixed Methods Study

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Purpose Although not always achieved, complete chemotherapy‐induced nausea and vomiting (CINV) control is the conventional goal of CINV prophylaxis. In this two‐center, mixed‐methods study, we sought to understand the preferences of adolescent patients and family caregivers for CINV control endpoints.
Haley Newman, Tadi Hondonga, Alison Berry, Caitlin Elgarten, Jason Freedman, Katelyn Oranges, Sara McDonough, Caroline Diorio, L. Lee Dupuis   +8 more
wiley   +1 more source

Chacma baboon natural anticoagulants and factor VIII activities

Scientific Reports
Non-human primates are useful models of human haemostasis, but distinctions do exist. These differences are essential to identify and quantify to better characterise the Chacma baboon’s coagulation system.
Quintin Andre van Staden, Sarah Meiring Muriel, Conradie Charmaine, Joubert Jaco   +3 more
doaj   +1 more source

In vitro extracellular replication of Wolbachia endobacteria

Frontiers in Microbiology
Obligate intracellular endobacteria of the genus Wolbachia are widespread in arthropods and several filarial nematodes. Control programs for vector-borne diseases (dengue, Zika, malaria) and anti-filarial therapy with antibiotics are based on this ...
Lara Vanessa Behrmann, Kirstin Meier, Jennifer Vollmer, Chukwuebuka Chibuzo Chiedu, Andrea Schiefer, Achim Hoerauf, Achim Hoerauf, Kenneth Pfarr, Kenneth Pfarr   +8 more
doaj   +1 more source