Results 251 to 260 of about 1,526,120 (295)

Cell-Free Protein Synthesis Using Pichia pastoris

2022
Pichia pastoris (syn. Komagataella phaffii) is an industrially relevant recombinant protein platform that has been used to produce over 5000 proteins to date. Cell-free protein synthesis can be used as a screening tool before strain development or for the production of proteins that are difficult or toxic to make in vivo.
Alex J, Spice, Rochelle, Aw, Karen M, Polizzi   +2 more
openaire   +2 more sources

Methods for energizing cell-free protein synthesis

Journal of Bioscience and Bioengineering, 2009
The productivity of cell-free protein synthesis depends critically upon the efficiency of ATP regeneration. In addition, the expensive energy sources used in the conventional cell-free protein synthesis systems account for the major part of the overall cost for the expression of proteins.
Ho-Cheol, Kim, Dong-Myung, Kim
openaire   +2 more sources

Phosphatase-immunodepleted cell-free protein synthesis system

Journal of Biotechnology, 1998
A cell-free protein synthesis system using wheat-germ extract was improved by a novel approach involving selective removal of endogenous phosphatase, which reduces both the duration and the rate of translation by hydrolyzing ATP and GTP, from the translational reaction.
Y, Kawarasaki, H, Nakano, T, Yamane
openaire   +2 more sources

Cell-free protein synthesis on a microchip

PROTEOMICS, 2002
We evaluated the expression of various proteins by using a cell-free protein synthesis system with a rapid translation system, a microtube, and a microchip technique. Protein expression was successfully achieved with a microfabricated reaction chamber on a plastic chip.
Marl, Tabuchi, Mami, Hino, Yasuo, Shinohara, Yoshinobu, Baba   +3 more
openaire   +2 more sources

Cell-free synthesis of zinc-binding proteins

Journal of Structural and Functional Genomics, 2006
Cell-free protein synthesis has become one of the standard methods for protein expression. The cell-free method is suitable for the synthesis of a protein that requires a ligand for its enzymatic activity and/or structure formation and stabilization, since it is an open system, which allows us to add the proper ligand to the reaction mixture.
Takayoshi, Matsuda, Takanori, Kigawa, Seizo, Koshiba, Makoto, Inoue, Masaaki, Aoki, Kazuhiko, Yamasaki, Motoaki, Seki, Kazuo, Shinozaki, Shigeyuki, Yokoyama   +8 more
openaire   +2 more sources

Universal cell-free protein synthesis

Nature Biotechnology, 2009
Unstructured translation-initiation sequences enable protein synthesis in cell extracts from multiple organisms.
openaire   +1 more source

Cell-free protein synthesis

2020
Proteins are the main source of drug targets and some of them possess therapeutic potential themselves. Among them, membrane proteins constitute approximately 50% of the major drug targets. In the drug discovery pipeline, rapid methods for producing different classes of proteins in a simple manner with high quality are important for structural and ...
Dondapati, Srujan Kumar, Stech, Marlitt, Zemella, Anne (Dr. rer. nat.), Kubick, Stefan (Dr. rer. nat.)   +3 more
openaire   +1 more source

A novel cell-free protein synthesis system

Journal of Biotechnology, 2004
An efficient cell-free protein synthesis system has been developed using a novel energy-regenerating source. Using the new energy source, 3-phosphoglycerate (3-PGA), protein synthesis continues beyond 2 h. In contrast, the reaction rate slowed down considerably within 30-45 min using a conventional energy source, phosphoenol pyruvate (PEP) under ...
Kalavathy, Sitaraman, Dominic, Esposito, George, Klarmann, Stuart F, Le Grice, James L, Hartley, Deb K, Chatterjee   +5 more
openaire   +2 more sources

Cell-free protein synthesis for biocatalysis

Cell-free protein synthesis (CFPS) serves as an innovation booster in the field of biocatalysis. By integrating CFPS into the design and development of biocatalysts, the discovery, synthesis, and screening of previously untapped enzymes and their engineered variants can be improved.
Siranjeevi, Balakrishnan, Katrin, Rosenthal   +1 more
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Cell-Free Synthesis of Factor VIII Related Protein

Thrombosis and Haemostasis, 1983
SummaryPolyadenylated ribonucleic acid (RNA) was isolated from confluent human endothelial cells in culture and translated in a cell-free rabbit reticulocyte lysate protein-synthesizing system. Using specific immunopredpitation, the translation product was found to contain 1.58% VIH related protein (VIIIR), which has a molecular weight of 0.26 × 106 ...
Chan, V, Chan, TK
openaire   +3 more sources