Results 61 to 70 of about 1,526,120 (295)

Molecular bases of circadian magnesium rhythms across eukaryotes

FEBS Letters, EarlyView.
Circadian rhythms in intracellular [Mg2+] exist across eukaryotic kingdoms. Central roles for Mg2+ in metabolism suggest that Mg2+ rhythms could regulate daily cellular energy and metabolism. In this Perspective paper, we propose that ancestral prokaryotic transport proteins could be responsible for mediating Mg2+ rhythms and posit a feedback model ...
Helen K. Feord, Gerben van Ooijen
wiley   +1 more source

Cell-Free Metabolic Engineering: Recent Developments and Future Prospects

Methods and Protocols, 2019
Due to the ongoing crises of fossil fuel depletion, climate change, and environmental pollution, microbial processes are increasingly considered as a potential alternative for cleaner and more efficient production of the diverse chemicals required for ...
Hye Jin Lim, Dong-Myung Kim
doaj   +1 more source

Cell-free Synthesis of Adenovirus Coat Proteins

Journal of Biological Chemistry, 1974
Abstract Messenger RNA extracted from polysomes of KB cells at late stages of productive infection with adenovirus type 2 was translated in a cell-free system derived from Krebs II ascites cells. Four of the polypeptides obtained in this reaction corresponded to the virion coat components hexon, IIIa, penton base, and fiber. The following criteria were
L, Eron, R, Callahan, H, Westphal
openaire   +2 more sources

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source

High-Level Expression of Various Apolipoprotein (a) Isoforms by "Transferrinfection". The Role of Kringle IV Sequences in the Extracellular Association with Low-Density Lipoprotein [PDF]

, 1994
Characterization of the assembly of lipoprotein(a) [Lp(a)] is of fundamental importance to understanding the biosynthesis and metabolism of this atherogenic lipoprotein. Since no established cell lines exist that express Lp(a) or apolipoprotein(a) [apo(a)
Azrolan N., Boerwinkle E., Brunner C., Chiesa G., Chung B. Y., Cotten M., Deutscher S. L., Deutscher S. L., Dieplinger H., Edelberg J. M., Green S., Griffiths G., Guevara J., Jr., Huby T., Ichinose A., Kamboh M. I., Koschinsky M. L., Koschinsky M. L., Koschinsky M. L., Kostner G. M., Kraft H- G., Kraft H. G., Krempler F., Krempler F., Lackner C., Linton M. F., Malgaretti N., McGregor G. R., McLean J. W., Menzel H. J., Phillips M. L., Rader D. J., Sambrook J., Sandholzer C., Scanu A. M., Selden R. F., Sommer A., Stanley P., Trieu V. N., Utermann G., Utermann G., Utermann G., Wagner E., White A. L., Zatloukal K., Zechner R„   +45 more
core   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Hair Root Cell-Free Protein Synthesis

Journal of Investigative Dermatology, 1970
A cell-free amino acid incorporating system has been prepared from mammalian hair root cells obtained by epilating animals with latex paint. The system requires a particulate fraction from the tissue homogenate (microsomes or ribosomes), a supernatant fraction in the case of ribosomes, ATP, GTP and magnesium.
openaire   +2 more sources

Function‐driven design of a surrogate interleukin‐2 receptor ligand

FEBS Letters, EarlyView.
Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang, Zelin Cheng, Teng Li, Fulian Wang, Liangminghui Zhang, Xiuxiu He, Lili Liu, Wei Wang, Aibin Liang, Guang Yang   +9 more
wiley   +1 more source

Time after time – circadian clocks through the lens of oscillator theory

FEBS Letters, EarlyView.
Oscillator theory bridges physics and circadian biology. Damped oscillators require external drivers, while limit cycles emerge from delayed feedback and nonlinearities. Coupling enables tissue‐level coherence, and entrainment aligns internal clocks with environmental cues.
Marta del Olmo, Carolin Ector, Hanspeter Herzel   +2 more
wiley   +1 more source

Silk fibroin as an additive for cell-free protein synthesis

Synthetic and Systems Biotechnology, 2020
Cell-free systems contain many proteins and metabolites required for complex functions such as transcription and translation or multi-step metabolic conversions.
Marilyn S. Lee, Chia-Suei Hung, Daniel A. Phillips, Chelsea C. Buck, Maneesh K. Gupta, Matthew W. Lux   +5 more
doaj   +1 more source