Results 251 to 260 of about 4,355,748 (278)
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Analysis of apoptosis in cell-free systems
Methods, 2008Cell-free systems have been instrumental in the identification of several important components of the cell death machinery such as cytochrome c, APAF-1, ICAD/CAD (DFF45/DFF40) and Smac/Diablo. Such systems have also proved invaluable for the detailed analysis of caspase activation mechanisms, caspase activation cascades, proteolysis of caspase ...
Sean P, Cullen +2 more
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Yeast Cell-Free Translation Systems
2002Translation in eukaryotes is well conserved among higher eukaryotes, such as mammals, and in lower eukaryotes, such as the yeast Saccharomyces cerevisiae. Since yeast cells are easy to handle and amenable to very powerful genetic and biochemical analysis, the yeast system represents an attractive model system for studies on the mechanism and regulation
Michael Altmann, Hans Trachsel
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Phosphatase-immunodepleted cell-free protein synthesis system
Journal of Biotechnology, 1998A cell-free protein synthesis system using wheat-germ extract was improved by a novel approach involving selective removal of endogenous phosphatase, which reduces both the duration and the rate of translation by hydrolyzing ATP and GTP, from the translational reaction.
Y, Kawarasaki, H, Nakano, T, Yamane
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In silico assessment of cell‐free systems
Biotechnology and Bioengineering, 2012AbstractCell‐free extract (CFX)‐derived biocatalytic systems are usually embedded in a complex metabolic network, which makes chemical insulation of the production system necessary by removing enzymatic connections. While insulation can be performed by different methods, the identification of potentially disturbing reactions can become a rather lengthy
Matthias, Bujara, Sven, Panke
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Cell-Free Production Systems in Droplet Microfluidics
2023The use of cell-free production systems in droplet microfluidic devices has gained significant interest during the last decade. Encapsulating DNA replication, RNA transcription, and protein expression systems in water-in-oil drops allows for the interrogation of unique molecules and high-throughput screening of libraries of industrial and biomedical ...
Rémi, Sieskind +2 more
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Membrane Protein Expression in Cell-Free Systems
2009Cell-free expression has emerged as a promising tool for the fast and efficient production of membrane proteins. The rapidly growing number of successfully produced targets in combination with the continuous development of new applications significantly promotes the distribution of this technology.
Birgit, Schneider +6 more
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Photosynthesis in cell-free systems
Journal of Chemical Education, 1956Examines chloroplasts and the Hill reaction, the fixation and reduction of carbon dioxide, the photochemical decomposition of DPNH and ATP, the carbon dioxide acceptor system, and photosynthesis in...
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Reconstituted AIM2 inflammasome in cell-free system
Journal of Immunological Methods, 2015Absent in melanoma 2 (AIM2) is an intracellular pattern-recognition receptor, which is a member of the PYHIN protein family, consisting of a PYD domain and an IFN-inducible nuclear localization (HIN) domain. AIM2 is reported to oligomerize with adaptor protein ASC upon sensing bacterial and viral cytosolic DNA in order to form the AIM2 inflammasome ...
Naoe, Kaneko +11 more
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Enterovirus eclipse in a cell-free system
Virology, 1964Abstract When purified P32-labeled ECHO 7 virus is centrifuged at high speed, a RNase-susceptible P32-labeled compound is released. The interaction of purified but not centrifuged virus and erythrocyte receptor substance was studied. Irreversible attachment to soluble and purified receptor from human erythrocyte membranes appears to be enzymatic ...
L, PHILIPSON, M, LIND
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Heterologous and Cell-Free Protein Expression Systems
2009In recognition of the fact that a relatively small percentage of 'named' genes in databases have any experimental proof for their annotation, attention is shifting towards the more accurate assignment of functions to individual genes in a genome. The central objective will be to reduce our reliance on nucleotide or amino acid sequence similarities as a
Farrokhi, N. +3 more
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