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A novel cell-free protein synthesis system
Journal of Biotechnology, 2004An efficient cell-free protein synthesis system has been developed using a novel energy-regenerating source. Using the new energy source, 3-phosphoglycerate (3-PGA), protein synthesis continues beyond 2 h. In contrast, the reaction rate slowed down considerably within 30-45 min using a conventional energy source, phosphoenol pyruvate (PEP) under ...
Kalavathy, Sitaraman +5 more
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Cell‐free translation systems for protein engineering
The FEBS Journal, 2006Cell‐free translation systems have developed significantly over the last two decades and improvements in yield have resulted in their use for protein production in the laboratory. These systems have protein engineering applications, such as the production of proteins containing unnatural amino acids and development of proteins exhibiting novel ...
Yoshihiro, Shimizu +4 more
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Development of Cell-Free Systems
2013Originally, work on the biosynthesis of protochlorophyll(ide) [Pchl(ide)] and chlorophyll (Chl) in organello, started in 1967 in my laboratory at the National Research Institute in Tel-el-Amara, Lebanon (see Chap. 2) (Rebeiz 1967, 1968). At the time spectrophotometric instrumentation was used.
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Roundtable on Cell-Free Systems
1992Almost every step in membrane traffic in the endocytic pathway has now been reconstituted in cell-free systems. This workshop included presentations by Mark Pypaert and Sandra Schmid on the formation of coated vesicles from coated pits, by Jean Gruenberg on the fusion of early and late endosomes, and by Paul Luzio and Robert Murphy both studying fusion
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Cell-free protein synthesis system
The technology provided herein relates to novel methods and systems for cell-free protein synthesis, in particular to cell-free protein synthesis systems suitable for protein synthesis before and after freezing for a short- or long-term storage, wherein said system comprises a cryoprotectant.Buntru, Matthias +2 more
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Gene expression using cell-free systems
Current Opinion in Biotechnology, 1990openaire +2 more sources
Immunoglobulin synthesis in a cell-free system
Biochemistry, 1971P, Ralph, A, Rich
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Cell-Free System Synthesizes Enzyme
Chemical & Engineering News Archive, 1961openaire +1 more source