Results 341 to 350 of about 6,347,699 (399)

Cell free systems for biodesign

Cell-free systems (CFS) have emerged as transformative tools in synthetic biology, enabling the execution of complex biological reactions such as transcription and translation outside the confines of living cells. By eliminating the cellular membrane, CFS allows unprecedented control over biochemical conditions, facilitating rapid prototyping (up to ...
Mohd, Tariq, Nil, Patil, Mukul, Jain, Dhruv, Desai, Piyusha, Kuhite, Ayush, Madan, Sandeep, Rawat   +6 more
openaire   +2 more sources

The biosynthesis of all major pea gibberellins in a cell-free system from Pisum sativum

Phytochemistry, 1983
Y. Kamiya, J. Graebe
semanticscholar   +3 more sources

Computational biology for cell-free systems

Cell-free systems (CFS) decouple gene expression and metabolic pathways from living cells, offering a rapid, modular platform for biosensing, pathway prototyping, and protein production. This review surveys mechanistic and data-driven computational approaches tailored to CFS design and optimization.
Mansi, Acharya, Indra, Mani
openaire   +2 more sources

Systems for the Cell-Free Synthesis of Proteins

2011
We describe a system for the cell-free expression of proteins based on extracts from Escherichia coli. Two reaction configurations, batch and continuous exchange, are discussed and analytical scale as well as preparative scale setups are documented. Guidelines for the systematic development and optimization of cell-free expression protocols are given ...
Lei, Kai, Christian, Roos, Stefan, Haberstock, Davide, Proverbio, Yi, Ma, Friederike, Junge, Mikhail, Karbyshev, Volker, Dötsch, Frank, Bernhard   +8 more
openaire   +2 more sources

Cell-free systems for automation and robotics

The amalgamation of cell-free systems with automation and robotics is transforming the domains of biotechnology and bioengineering. This chapter examines the swiftly advancing domain of robotic platforms utilizing cell-free protein synthesis (CFPS) and other programmable cell-free biological mechanisms.
Juveriya, Israr, Shabroz, Alam, Ajay, Kumar   +2 more
openaire   +2 more sources

Cell-Free Systems for the Production of Glycoproteins

Cell-free protein synthesis (CFPS), whereby cell lysates are used to produce proteins from a genetic template, has matured as an attractive alternative to standard biomanufacturing modalities due to its high volumetric productivity contained within a distributable platform.
Erik J, Bidstrup, Yong Hyun, Kwon, Keehun, Kim, Chandra Kanth, Bandi, Rochelle, Aw, Michael C, Jewett, Matthew P, DeLisa   +6 more
openaire   +2 more sources

Artificial intelligence for cell-free systems

Cell-free systems let researchers carry out biological processes like protein synthesis and metabolism without using living cells. This approach has become increasingly important in synthetic biology because it allows for quick testing of ideas, running many experiments simultaneously, and maintaining tight control over reaction conditions.
Ingita Dey, Munshi, Indra, Mani
openaire   +2 more sources

Reconstituted AIM2 inflammasome in cell-free system

Journal of Immunological Methods, 2015
Absent in melanoma 2 (AIM2) is an intracellular pattern-recognition receptor, which is a member of the PYHIN protein family, consisting of a PYD domain and an IFN-inducible nuclear localization (HIN) domain. AIM2 is reported to oligomerize with adaptor protein ASC upon sensing bacterial and viral cytosolic DNA in order to form the AIM2 inflammasome ...
Naoe, Kaneko, Yuki, Ito, Tomoyuki, Iwasaki, Hiroyuki, Takeda, Tatsuya, Sawasaki, Kiyoshi, Migita, Kazunaga, Agematsu, Atsushi, Kawakami, Shinnosuke, Morikawa, Sho, Mokuda, Mie, Kurata, Junya, Masumoto   +11 more
openaire   +2 more sources

Potentiation of Apoptosis by Mitochondria in a Cell-Free System

Biochemical and Biophysical Research Communications, 1998
Using a cell-free system, we show that rat liver mitochondria, but not mitochondrial extracts, potentiated apoptosis triggered by cytosols derived from apoptotic cells. Apoptosis potentiated by mitochondria appeared to be inhibited by caspase 3 but not by caspase 1 inhibitors.
P, Juin, K, Tremblais, M T, LeCabellec, M, Grégoire, K, Meflah, F M, Vallette   +5 more
openaire   +2 more sources

Generation of a bioactive neuropeptide in a cell-free system

Analytical Biochemistry, 2003
We have developed an in vitro assay for pre-pro-neuropeptide synthesis and processing. Mouse proopiomelanocortin (POMC) cDNA was cloned into a vector containing T7 promoter. In vitro transcription and translation were carried out to produce the proopiomelanocortin peptide.
Cecilia, Jiang, Guifen, Chen, Xuemei, Zeng, Keqing, Ouyang, Yinghe, Hu   +4 more
openaire   +2 more sources