Results 351 to 360 of about 6,347,699 (399)
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A bio-inspired cell-free system for cannabinoid production from inexpensive inputs
Nature Chemical Biology, 2020Meaghan A. Valliere +3 more
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Cell-free systems for development of biosensors
Cell-free systems (CFSs) have become powerful tools in synthetic biology, enabling the creation of fast, modular, and customizable biosensors without relying on living cells. By utilizing in vitro transcription and translation, these systems offer a finely controlled biochemical environment suitable for sensing applications in fields such as healthcare,Stuti, Ganatra, Alok, Pandya
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Protocol for preparation of cell–free system
Cell-free protein synthesis (CFPS) has emerged as a revolutionary process for producing proteins quickly and in a modular way, with the optionality of not having all of the restraints of living cells. As described in this chapter, we provide full protocols for preparing cell extracts with high activity, optimizing the reaction systems for protein ...B G, Pavana +4 more
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Synthesis of metallothionein in a polysomal cell-free system
Biochemical and Biophysical Research Communications, 1977Abstract A new covalent chromatography system utilizing Activated Thiol Sepharose 4B was employed to quantitate the content of thionein chains synthesized in a polysomal cell-free system. Liver polysomes from zinc injected rats directed the translation of more thionein-like polypeptide chains than polysomes from control rats. The increase was similar
K S, Squibb, R J, Cousins
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2006
A disrupted cell is capable of synthesizing protein from amino acids depending upon the template RNA. This discovery, half a century ago, set the stage for biochemical studies on the gene expression process occurring in cells. The cell-free translation system successfully revealed the function of a number of key molecules participating in the ...
Takuya Ueda +2 more
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A disrupted cell is capable of synthesizing protein from amino acids depending upon the template RNA. This discovery, half a century ago, set the stage for biochemical studies on the gene expression process occurring in cells. The cell-free translation system successfully revealed the function of a number of key molecules participating in the ...
Takuya Ueda +2 more
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Membrane Protein Expression in Cell-Free Systems
2009Cell-free expression has emerged as a promising tool for the fast and efficient production of membrane proteins. The rapidly growing number of successfully produced targets in combination with the continuous development of new applications significantly promotes the distribution of this technology.
Birgit, Schneider +6 more
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Photosynthesis in cell-free systems
Journal of Chemical Education, 1956Examines chloroplasts and the Hill reaction, the fixation and reduction of carbon dioxide, the photochemical decomposition of DPNH and ATP, the carbon dioxide acceptor system, and photosynthesis in...
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Roundtable on Cell-Free Systems
1992Almost every step in membrane traffic in the endocytic pathway has now been reconstituted in cell-free systems. This workshop included presentations by Mark Pypaert and Sandra Schmid on the formation of coated vesicles from coated pits, by Jean Gruenberg on the fusion of early and late endosomes, and by Paul Luzio and Robert Murphy both studying fusion
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2002
I Introduction.- 1 Cell-Free Protein Synthesis.- II Improved Classical (Batch) Cell-Free Translation Systems.- 2 Improved Batch Translation System Based on E. coli Extract.- 3 Improved Composition and Energy Supply for Bacterial Batch Systems.- 4 PURE System: New Cell-Free Translation System Reconstructed with Purified Components.- 5 Direct Expression ...
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I Introduction.- 1 Cell-Free Protein Synthesis.- II Improved Classical (Batch) Cell-Free Translation Systems.- 2 Improved Batch Translation System Based on E. coli Extract.- 3 Improved Composition and Energy Supply for Bacterial Batch Systems.- 4 PURE System: New Cell-Free Translation System Reconstructed with Purified Components.- 5 Direct Expression ...
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Structural and physico-mechanical characterization of bio-cellulose produced by a cell-free system.
Carbohydrate Polymers, 2016M. W. Ullah +4 more
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