Molluscan cells in culture: primary cell cultures and cell lines [PDF]
In vitro cell culture systems from molluscs have significantly contributed to our basic understanding of complex physiological processes occurring within or between tissue-specific cells, yielding information unattainable using intact animal models.
U Bickham+2 more
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A Novel 2.5D Culture Platform to Investigate the Role of Stiffness Gradients on Adhesion-Independent Cell Migration [PDF]
Current studies investigating the role of biophysical cues on cell migration focus on the use of culture platforms with static material parameters. However, migrating cells in vivo often encounter spatial variations in extracellular matrix stiffness.
Asuri, Prashanth+2 more
core +6 more sources
T Cell Development in Culture [PDF]
The T cell compartment is continuously replenished by a renewable source of stem cells. In the adult, bone marrow-derived stem cells seed the thymus and initiate a developmental program that requires a series of incompletely understood signals that are normally provided by the thymus. Failure to recapitulate this process in simple in vitro cultures has
Michael J. Bevan, Sophie M. Lehar
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Cell-Culture Measurements Using Voltage Oscillations [PDF]
A comprehensive system for real-time monitoring of a set of cell-cultures using a Voltage Oscillation (VO) methodology is proposed. The main idea is to connect the bio-electrical elements (electrodes & cell-culture) in such a way that sequentially a ...
Huertas Sánchez, Gloria+5 more
core +1 more source
Expression of Tumor Assosiated and Epithelial-mesenchymal Transition Markers in 2d and 3d Cell Cultures of Mcf-7 [PDF]
The target effects on the expression of epithelial-mesenchymal transition regulation molecules are promising for cancer therapy, including breast cancer.
Herheliuk, T. (Tetiana)+4 more
core +2 more sources
A new cell primo-culture method for freshwater benthic diatom communities [PDF]
A new cell primo-culture method was developed for the benthic diatom community isolated from biofilm sampled in rivers. The approach comprised three steps: (1) scraping biofilm from river pebbles, (2) diatom isolation from biofilm, and (3) diatom ...
A Berard+34 more
core +3 more sources
Improving single-cell cloning workflow for gene editing in human pluripotent stem cells [PDF]
The availability of human pluripotent stem cells (hPSCs) and progress in genome engineering technology have altered the way we approach scientific research and drug development screens.
Chen, Yi-Hsien, Pruett-Miller, Shondra M
core +2 more sources
The ePetri dish, an on-chip cell imaging platform based on subpixel perspective sweeping microscopy (SPSM) [PDF]
We report a chip-scale lensless wide-field-of-view microscopy imaging technique, subpixel perspective sweeping microscopy, which can render microscopy images of growing or confluent cell cultures autonomously.
Biener+29 more
core +3 more sources
A miniaturized bioreactor system for the evaluation of cell interaction with designed substrates in perfusion culture [PDF]
In tissue engineering, the chemical and topographical cues within three-dimensional (3D) scaffolds are normally tested using static cell cultures but applied directly to tissue cultures in perfusion bioreactors.
Anderson+31 more
core +2 more sources
Microsystems for Cell Cultures
Microfabricated systems are increasingly being utilized in biotechnological, biomedical, and pharmaceutical research and development as replacements for traditional in vitro cell cultures, bioreactors, and animal experiments (Figure 1) [...]
openaire +3 more sources