Results 1 to 10 of about 3,334,633 (288)

Presence of pyrroloquinazoline alkaloid in Adhatoda vasica attenuates inflammatory response through the downregulation of pro-inflammatory mediators in LPS stimulated RAW 264.7 macrophages [PDF]

BioImpacts, 2021
Introduction: Inflammation is the primary response caused due to harmful stimuli which are followed by the increased draining of plasma and immune cells from the body into the site of the injured tissue.
Reddy Amala, Sundaresan Sujatha
doaj   +1 more source

Interplay of heavy chain introns influences efficient transcript splicing and affects product quality of recombinant biotherapeutic antibodies from CHO cells

mAbs, 2023
Introns are included in genes encoding therapeutic proteins for their well-documented function of boosting expression. However, mis-splicing of introns in recombinant immunoglobulin (IgG) heavy chain (HC) transcripts can produce amino acid sequence ...
Emma Kelsall, Claire Harris, Titash Sen, Diane Hatton, Sarah Dunn, Suzanne Gibson   +5 more
doaj   +1 more source

Gene Expression and Immunochemistry Analysis of ADAMTS-1 and Versican in Ameloblastoma

International Journal of Dentistry, 2022
Background. Ameloblastoma is a benign but locally invasive odontogenic epithelial tumor, associated with a high recurrence rate after treatment. The action of enzymes of the metalloproteinase family is important to the degraded extracellular matrix ...
Osvaldo Rodrigues de Souza Neto, Hellen Thais Fuzii, Suély Vieira Da Silva, Vanessa Morais Freitas, João de Jesus Viana Pinheiro   +4 more
doaj   +1 more source

Key proteins of invadopodia are overexpressed in oral squamous cell carcinoma suggesting an important role of MT1-MMP in the tumoral progression

Diagnostic Pathology, 2021
Background Oral squamous cell carcinoma (OSCC) is the most relevant malignant neoplasm among all head and neck tumours due to its high prevalence and unfavourable prognosis.
Geovanni Pereira Mitre, Karolyny Martins Balbinot, André Luis Ribeiro Ribeiro, Maria Sueli da Silva Kataoka, Sérgio de Melo Alves Júnior, João de Jesus Viana Pinheiro   +5 more
doaj   +1 more source

Perfusion reduces bispecific antibody aggregation via mitigating mitochondrial dysfunction-induced glutathione oxidation and ER stress in CHO cells

Scientific Reports, 2020
One major challenge observed for the expression of therapeutic bispecific antibodies (BisAbs) is high product aggregates. Aggregates increase the risk of immune responses in patients and therefore must be removed at the expense of purification yields ...
Pritam Sinharoy, Aaron H. Aziz, Natalia I. Majewska, Sanjeev Ahuja, Michael W. Handlogten   +4 more
doaj   +1 more source

Preservation media, durations and cell concentrations of short-term storage affect key features of human adipose-derived mesenchymal stem cells for therapeutic application [PDF]

PeerJ, 2017
Background Adipose-derived mesenchymal stem cells (ADSCs) have shown great potential in the treatment of various diseases. However, the optimum short-term storage condition of ADSCs in 2∼8 °C is rarely reported.
Fengli Zhang, Huaijuan Ren, Xiaohu Shao, Chao Zhuang, Yantian Chen, Nianmin Qi   +5 more
doaj   +2 more sources

Heterogeneity of mesenchymal stem cell-derived extracellular vesicles is highly impacted by the tissue/cell source and culture conditions

Cell & Bioscience, 2022
Extracellular vesicles (EVs) are cell-derived membrane structures exerting major effects in physiological as well as pathological processes by functioning as vehicles for the delivery of biomolecules to their target cells. An increasing number of effects
Ciarra Almeria, Sebastian Kreß, Viktoria Weber, Dominik Egger, Cornelia Kasper   +4 more
doaj   +1 more source

HIF-1α Is Associated with Resistance to Hypoxia-Induced Apoptosis in Ameloblastoma

International Journal of Dentistry, 2021
Background. Ameloblastoma (AMB) is a benign odontogenic tumour, with an aggressive local behaviour and a high rate of recurrence. Previous studies have demonstrated that hypoxia-induced factor alpha 1 (HIF-1α) and activated caspase-3 contribute to tumour
Katherine Julissa Palma Valladares, Karolyny Martins Balbinot, Antonia Taiane Lopes de Moraes, Maria Sueli da Silva Kataoka, Aline Maria Pereira Cruz Ramos, Rommel Thiago Jucá Ramos, Artur Luiz da Costa da Silva, Ricardo Alves Mesquita, David Normando, Sérgio de Melo Alves Júnior, João de Jesus Viana Pinheiro   +10 more
doaj   +1 more source

Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli

AMB Express, 2017
Secretion of heterologous proteins into Escherichia coli cell culture medium offers significant advantages for downstream processing over production as inclusion bodies; including cost and time savings, and reduction of endotoxin. Signal peptides play an
SooJin Han, Shushil Machhi, Mark Berge, Guoling Xi, Thomas Linke, Ronald Schoner   +5 more
doaj   +1 more source

Effect of lyophilization on HRP–antibody conjugation: an enhanced antibody labeling technology

BMC Research Notes, 2018
Objective Immunoassay usually deal with the antibody labeling with various reporter molecules, one such useful reporter molecule is horseradish peroxidase (HRPO).
Saikant Regidi, Shilpa Ravindran, Ashitha L. Vijayan, Vani Maya, Lakshmi Sreedharan, Jeslin Varghese, Kartik Ramaswami, Manoj Gopi   +7 more
doaj   +1 more source