Effect of lyophilization on HRP–antibody conjugation: an enhanced antibody labeling technology
Objective Immunoassay usually deal with the antibody labeling with various reporter molecules, one such useful reporter molecule is horseradish peroxidase (HRPO).
Saikant Regidi +7 more
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The Hollow Porous Sphere Cell Carrier for the Dynamic Three-Dimensional Cell Culture [PDF]
Large-scale mammalian cell culture is essential in cell therapy, vaccine production, and the manufacturing of therapeutic protein drugs. Due to the adherent growth characteristic of most mammalian cell types, the combination of cell carrier and ...
Yarlagadda, Prasad +18 more
core +1 more source
Cell cycle analysis of primary sponge cell cultures [PDF]
Proliferation of sponge cells is generally measured via cell counts or viability assays. However, more insight into the proliferative state of a sponge cell population can be obtained from the distribution of the cells over the different phases of the ...
Pomponi, S.A. +7 more
core +1 more source
For years, the renin-angiotensin system (RAS) has been perceived as a system whose role is to primarily modulate the functioning of the cardiovascular system.
Maksymilian Ziaja +3 more
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Vascularization in 3D Cell Culture
The two-dimensional (2D) monoculture does not recapitulate the three-dimensional (3D) environment of in vivo vasculature. Endothelial cell (ECs) spheroids have been introduced as a 3D in vitro model exhibiting angiogenic responses and sprouting behavior ...
Bagli, E. +9 more
core +1 more source
Engineered CHO cells as a novel AAV production platform for gene therapy delivery
The Herpes simplex virus (HSV)-based platform for production of recombinant adeno-associated viral vectors (rAAVs) yields higher titers and increased percentage of full capsids when compared to the triple transient transfection (TTT) method.
Abdou Nagy +7 more
doaj +1 more source
A miniaturized bioreactor system for the evaluation of cell interaction with designed substrates in perfusion culture [PDF]
In tissue engineering, the chemical and topographical cues within three-dimensional (3D) scaffolds are normally tested using static cell cultures but applied directly to tissue cultures in perfusion bioreactors.
M. O. Riehle +17 more
core +1 more source
The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator [PDF]
Heidemann R, Riese U, Lütkemeyer D, Büntemeyer H, Lehmann J. The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator. Cytotechnology.
Heidemann, R. +4 more
core +1 more source
Occurrence of spontaneous polyploids in Citrus
Seedlings recovered from underdeveloped seeds of different commercial cultivars of Citrus were studied for their ploidy level. Cytological examination revealed that maximum triploid plants were found in lime Kaghzi (15.5%) followed by grapefruit Foster ...
M. Usman +3 more
doaj +1 more source
Comparative Antimicrobial Effects of Rat Blood-derived Products and Adipose-derived Mesenchymal Stem Cell Conditioned Medium [PDF]
Background: The antimicrobial characteristics of biological products are fundamental in medicine due to their ability to treat microbial infections. This research aimed to assess and compare the antimicrobial properties of various platelet concentrates ...
Mohsen Sheykhhasan +3 more
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