Results 11 to 20 of about 17,428,920 (362)

3D cell culture models: Drug pharmacokinetics, safety assessment, and regulatory consideration

Clinical and Translational Science, 2021
Nonclinical testing has served as a foundation for evaluating potential risks and effectiveness of investigational new drugs in humans. However, the current two‐dimensional (2D) in vitro cell culture systems cannot accurately depict and simulate the rich
Hongbing Wang, Paul C. Brown, E. Chow, L. Ewart, Steve S. Ferguson, S. Fitzpatrick, Benjamin S. Freedman, G. Guo, W. Hedrich, S. Heyward, J. Hickman, N. Isoherranen, Albert P. Li, Qi Liu, S. Mumenthaler, J. Polli, W. Proctor, A. Ribeiro, Jian‐Ying Wang, R. Wange, Shiew-Mei Huang   +20 more
semanticscholar   +1 more source

Vascularization in 3D Cell Culture [PDF]

, 2021
The two-dimensional (2D) monoculture does not recapitulate the three-dimensional (3D) environment of in vivo vasculature. Endothelial cell (ECs) spheroids have been introduced as a 3D in vitro model exhibiting angiogenic responses and sprouting behavior ...
Bagli, E., Murphy, C., D. Kouroupis, E. Bagli, M. Markou, Kouroupis, D., C. Murphy, Fotsis, T., Markou, M., T. Fotsis   +9 more
core   +1 more source

Key proteins of invadopodia are overexpressed in oral squamous cell carcinoma suggesting an important role of MT1-MMP in the tumoral progression

Diagnostic Pathology, 2021
Background Oral squamous cell carcinoma (OSCC) is the most relevant malignant neoplasm among all head and neck tumours due to its high prevalence and unfavourable prognosis.
Geovanni Pereira Mitre, Karolyny Martins Balbinot, André Luis Ribeiro Ribeiro, Maria Sueli da Silva Kataoka, Sérgio de Melo Alves Júnior, João de Jesus Viana Pinheiro   +5 more
doaj   +1 more source

The Hollow Porous Sphere Cell Carrier for the Dynamic Three-Dimensional Cell Culture [PDF]

, 2022
Large-scale mammalian cell culture is essential in cell therapy, vaccine production, and the manufacturing of therapeutic protein drugs. Due to the adherent growth characteristic of most mammalian cell types, the combination of cell carrier and ...
Yarlagadda, Prasad, Xiao, L, Xiao, Lan, Wang, Jiaqiu, Wu, Chengtie, Xiao, Yin, Mendhi, Jayanti, Gao, Weidong, Wu, C, Mu, Y, Mendhi, J, Li, Zhiyong, Wang, J, Yarlagadda, P, Mu, Yuqing, Li, Z, Gao, Wendong, Gao, W, Xiao, Y   +18 more
core   +1 more source

Perfusion reduces bispecific antibody aggregation via mitigating mitochondrial dysfunction-induced glutathione oxidation and ER stress in CHO cells

Scientific Reports, 2020
One major challenge observed for the expression of therapeutic bispecific antibodies (BisAbs) is high product aggregates. Aggregates increase the risk of immune responses in patients and therefore must be removed at the expense of purification yields ...
Pritam Sinharoy, Aaron H. Aziz, Natalia I. Majewska, Sanjeev Ahuja, Michael W. Handlogten   +4 more
doaj   +1 more source

Cell isolation and culture [PDF]

WormBook, 2013
Cell isolation and culture are essential tools for the study of cell function. Isolated cells grown under controlled conditions can be manipulated and imaged at a level of resolution that is not possible in whole animals or even tissue explants. Recent advances have allowed for large-scale isolation and culture of primary C.
Sihui, Zhang, Jeffrey R, Kuhn
openaire   +2 more sources

Preservation media, durations and cell concentrations of short-term storage affect key features of human adipose-derived mesenchymal stem cells for therapeutic application [PDF]

PeerJ, 2017
Background Adipose-derived mesenchymal stem cells (ADSCs) have shown great potential in the treatment of various diseases. However, the optimum short-term storage condition of ADSCs in 2∼8 °C is rarely reported.
Fengli Zhang, Huaijuan Ren, Xiaohu Shao, Chao Zhuang, Yantian Chen, Nianmin Qi   +5 more
doaj   +2 more sources

Heterogeneity of mesenchymal stem cell-derived extracellular vesicles is highly impacted by the tissue/cell source and culture conditions

Cell & Bioscience, 2022
Extracellular vesicles (EVs) are cell-derived membrane structures exerting major effects in physiological as well as pathological processes by functioning as vehicles for the delivery of biomolecules to their target cells. An increasing number of effects
Ciarra Almeria, Sebastian Kreß, Viktoria Weber, Dominik Egger, Cornelia Kasper   +4 more
doaj   +1 more source

Stable Isotope Labeling by Amino Acids in Cell Culture, SILAC, as a Simple and Accurate Approach to Expression Proteomics*

Molecular & Cellular Proteomics, 2002
Quantitative proteomics has traditionally been performed by two-dimensional gel electrophoresis, but recently, mass spectrometric methods based on stable isotope quantitation have shown great promise for the simultaneous and automated identification and ...
Shao-En Ong, Blagoy Blagoev, I. Kratchmarova, D. B. Kristensen, H. Steen, A. Pandey, M. Mann   +6 more
semanticscholar   +1 more source

Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli

AMB Express, 2017
Secretion of heterologous proteins into Escherichia coli cell culture medium offers significant advantages for downstream processing over production as inclusion bodies; including cost and time savings, and reduction of endotoxin. Signal peptides play an
SooJin Han, Shushil Machhi, Mark Berge, Guoling Xi, Thomas Linke, Ronald Schoner   +5 more
doaj   +1 more source