Results 111 to 120 of about 15,168,841 (359)

Thermal proteome profiling and proteome analysis using high‐definition mass spectrometry demonstrate modulation of cholesterol biosynthesis by next‐generation galeterone analog VNPP433‐3β in castration‐resistant prostate cancer

Molecular Oncology, EarlyView.
Elevated level of cholesterol is positively correlated to prostate cancer development and disease severity. Cholesterol‐lowering drugs, such as statins, are demonstrated to inhibit prostate cancer. VNPP433‐3β interrupts multiple signaling and metabolic pathways, including cholesterol biosynthesis, AR‐mediated transcription of several oncogenes, mRNA 5′
Retheesh S. Thankan, Elizabeth Thomas, Mehari M. Weldemariam, Puranik Purushottamachar, Weiliang Huang, Maureen A. Kane, Yuji Zhang, Nicholas Ambulos, Bi‐Dar Wang, David Weber, Vincent C. O. Njar   +10 more
wiley   +1 more source

Extracellular matrix of lung scaffolds submitted to different means of sterilization: a systematic review [version 1; peer review: 1 approved, 2 approved with reservations]

F1000Research
Chronic respiratory diseases often necessitate lung transplantation due to irreversible damage. Organ engineering offers hope through stem cell-based organ generation. However, the crucial sterilization step in scaffold preparation poses challenges. This
Luis Vicente Franco Oliveira, Giuseppe Insalaco, Renata K. Palma, Rodolfo P. Vieira, Deise A. A. P. Oliveira, Rodrigo F. Oliveira, Iransé Oliveira-Silva, Miria C. Oliveira, Diego A. C. P. G. Mello, Andressa D. Cereta, Adriano L. Fonseca, Joao Pedro R. Afonso, Ricardo S. Moura   +12 more
doaj  

Quantitative assessment of adipocyte differentiation in cell culture

Adipocyte, 2016
Adipocyte cell culture is an important tool for mechanistic studies of energy metabolism. Many factors affect the differentiation of adipocytes in culture. Oil red O staining can be used to assess the degree of differentiation.
Nils A Kraus, Franziska Ehebauer, B. Zapp, B. Rudolphi, B. Kraus, D. Kraus   +5 more
semanticscholar   +1 more source

Highly multiplexed digital PCR assay for simultaneous quantification of variant allele frequencies and copy number alterations of KRAS and GNAS in pancreatic cancer precursors

Molecular Oncology, EarlyView.
Combining melting curve analysis enhances the multiplexing capability of digital PCR. Here, we developed a 14‐plex assay to simultaneously measure single nucleotide mutations and amplifications of KRAS and GNAS, which are common driver genes in pancreatic cancer precursors. This assay accurately quantified variant allele frequencies in clinical samples
Junko Tanaka, Tatsuo Nakagawa, Yusuke Ono, Yoshio Kamura, Takeshi Ishida, Hidemasa Kawabata, Kenji Takahashi, Hiroki Sato, Andrew S. Liss, Yusuke Mizukami, Takahide Yokoi   +10 more
wiley   +1 more source

Gene expression and immunohistochemistry analysis of ADAMTS-1 and its substrates in odontogenic keratocyst

Diagnostic Pathology
Background Considering the significant participation of the microenvironment in the local aggressiveness of odontogenic keratocysts, this study aims to evaluate the expression of ADAMTS-1 and its substrates, versican, aggrecan and brevican in this ...
Osvaldo Rodrigues de Souza Neto, Antonia Taiane Lopes de Moraes, Hellen Thais Fuzii, Antonio Guilherme Maneschy Faria, Vanessa Morais Freitas, Maria Sueli da Silva Kataoka, Sérgio de Melo Alves, João de Jesus Viana Pinheiro   +7 more
doaj   +1 more source

ISOLATION AND STUDY OF MUTANTS FROM MAMMALIAN CELLS IN CULTURE [PDF]

, 1959
Irving Lieberman, Peter Ove
openalex   +1 more source

3D Cell Culture

Methods in Molecular Biology, 2017
3D cell culture is an invaluable tool in developmental, cell, and cancer biology. By mimicking crucial features of in vivo environment, including cell–cell and cell–extracellular matrix interactions, 3D cell culture enables proper structural architecture
Z. Koledova
semanticscholar   +1 more source

Exploration of heterogeneity and recurrence signatures in hepatocellular carcinoma

Molecular Oncology, EarlyView.
This study leveraged public datasets and integrative bioinformatic analysis to dissect malignant cell heterogeneity between relapsed and primary HCC, focusing on intercellular communication, differentiation status, metabolic activity, and transcriptomic profiles.
Wen‐Jing Wu, Jianchao Wang, Fuqing Chen, Xuefeng Wang, Bin Lan, Ruyi Fu, Hong Wen, Fangfang Chen, Wei Hong, Tian‐Yu Tang, Ying He, Gang Chen, Jianyin Zhou, Hai‐Long Piao, Di Chen, Shu‐Yong Lin   +15 more
wiley   +1 more source

THE EFFECT OF DILUTION OF PLASMA MEDIUM ON THE GROWTH AND FAT ACCUMULATION OF CELLS IN TISSUE CULTURES [PDF]

, 1914
Robert A. Lambert
openalex   +1 more source

Is it time to reinvent basic cell culture medium?

American Journal of Physiology - Cell Physiology, 2017
mammalian cell culture is an essential tool to examine cell function in a controlled environment. Cell culture relies on the assumption that the behavior of cells in vitro is fundamentally similar to their behavior as part of a tissue within an organ of ...
Turney J McKee, S. Komarova
semanticscholar   +1 more source