Results 131 to 140 of about 17,041,985 (363)

ManNAc attenuates Man5 glycoform abundance through GNE-mediated metabolic channeling of UDP-GlcNAc to N-glycosylation modifications via CMP-Neu5Ac biosynthesis

mAbs
N-glycosylation, a critical quality attribute of monoclonal antibodies, plays a pivotal role in regulating pharmacokinetics and pharmacodynamics through high-mannose (Man5) glycoform modulation.
Ruiqiang Sun, Miaomiao Chai, Jiahao Man, Guiju Yang, Hai Shu, Qiancheng Wang, Cong Tian, Linlin Wang, Shanhui Liao, Yifeng Zhang, Yanyan Cao, Lisha Xia, Haili Yang, Ting Zhang, Luoyan Ma, Shenwang Cao, Qiao Gao, Yichen Le, Mingli Shi, Quanxue Li, Yinmao Fan, Hang Zhou   +21 more
doaj   +1 more source

Traditional Chinese medicine herbal mixture LQ arrests FUCCI-expressing HeLa cells in G₀/G₁ phase in 2D plastic, 2.5D Matrigel, and 3D Gelfoam culture visualized with FUCCI imaging. [PDF]

, 2015
We used the fluorescence ubiquitination-based cell cycle indicator (FUCCI) to monitor cell cycle arrest after treatment of FUCCI-expressing HeLa cells (FUCCI-HeLa) with a traditional Chinese medicine (TCM) herbal mixture LQ, previously shown to have anti-
Bouvet, Michael, Hoffman, Robert M, Wu, Chengyu, Yano, Shuya, Zhang, Lei   +4 more
core   +2 more sources

Good Cell Culture Practice for stem cells and stem-cell-derived models.

ALTEX, 2016
The first guidance on Good Cell Culture Practice (GCCP) dates back to 2005. This document expands this to include aspects of quality assurance for in vitro cell culture focusing on the increasingly diverse cell types and culture formats used in research,
D. Pamies, A. Bal-Price, A. Simeonov, D. Tagle, Dave Allen, D. Gerhold, Dezhong Yin, F. Pistollato, T. Inutsuka, Kristie Sullivan, G. Stacey, H. Salem, M. Leist, Mardas Daneshian, M. Vemuri, R. Mcfarland, S. Coecke, S. Fitzpatrick, U. Lakshmipathy, Amanda A Mack, Wen Bo Wang, D. Yamazaki, Y. Sekino, Y. Kanda, Lena Smirnova, T. Hartung   +25 more
semanticscholar   +1 more source

Tumor clusters with divergent inflammation and human retroelement expression determine the clinical outcome of patients with serous ovarian cancer

Molecular Oncology, EarlyView.
Analysis of treatment‐naïve high‐grade serous ovarian carcinoma (HGSOC) and control tissues for ERVs, LINE‐1 (L1), inflammation, and immune checkpoints identified five clusters with diverse patient recurrence‐free survivals. An inflammation score was calculated and correlated with retroelement expression, where one novel cluster (Triple‐I) with high ...
Laura Glossner, Markus Eckstein, Christoph Mark, Matthias W. Beckmann, Arndt Hartmann, Pamela L. Strissel, Reiner Strick   +6 more
wiley   +1 more source

Semicontinuous Bioreactor Production of Recombinant Butyrylcholinesterase in Transgenic Rice Cell Suspension Cultures. [PDF]

, 2016
An active and tetrameric form of recombinant butyrylcholinesterase (BChE), a large and complex human enzyme, was produced via semicontinuous operation in a transgenic rice cell suspension culture.
Corbin, Jasmine M, Hashimoto, Bryce I, Karuppanan, Kalimuthu, Kyser, Zachary R, McDonald, Karen A, Nandi, Somen, Noe, Amy R, Roberts, Brian A, Rodriguez, Raymond L, Wu, Liying   +9 more
core   +2 more sources

Electroporation with Cisplatin against Metastatic Pancreatic Cancer:In VitroStudy on Human Primary Cell Culture [PDF]

, 2018
Olga Michel, Julita Kulbacka, Jolanta Saczko, Justyna Mączyńska, Piotr Błasiak, Joanna Rossowska, Adam Rzechonek   +6 more
openalex   +1 more source

β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1

Molecular Oncology, EarlyView.
Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero, Alejandro Belmonte‐Fernández, Mónica González‐Moreno, Laura Rodríguez‐Cordero, Begoña Pérez‐Valderrama, Joaquín Herrero‐Ruíz, Francisco Romero, Carmen Sáez, Miguel Á. Japón   +8 more
wiley   +1 more source

Interactions Between Fibroblast Cells and Laser Beam Welded AISI 2205 Duplex Stainless Steel

Medžiagotyra, 2018
Because of their high mechanical strength, excellent corrosion resistance and good weldability, duplex stainless steels are mostly used in industries such as oil, chemistry, petrochemistry, food and occasionally used in medical industry. These properties
Ceyhun KÖSE, Ramazan KAÇAR, Aslı Pınar ZORBA, Melahat BAĞIROVA, Emrah Şefik ABAMOR, Adil M. ALLAHVERDİYEV   +5 more
doaj   +1 more source

Nicotinamide N‐methyltransferase promotes drug resistance in lung cancer, as revealed by nascent proteomic profiling

Molecular Oncology, EarlyView.
AZD9291 has shown promise in targeted cancer therapy but is limited by resistance. In this study, we employed metabolic labeling and LC–MS/MS to profile time‐resolved nascent protein perturbations, allowing dynamic tracking of drug‐responsive proteins. We demonstrated that increased NNMT expression is associated with drug resistance, highlighting NNMT ...
Zhanwu Hou, Zhen Wang, Fei Yang, Xiao Han, Lei Li, Huadong Liu   +5 more
wiley   +1 more source

Gene expression and immunohistochemistry analysis of ADAMTS-1 and its substrates in odontogenic keratocyst

Diagnostic Pathology
Background Considering the significant participation of the microenvironment in the local aggressiveness of odontogenic keratocysts, this study aims to evaluate the expression of ADAMTS-1 and its substrates, versican, aggrecan and brevican in this ...
Osvaldo Rodrigues de Souza Neto, Antonia Taiane Lopes de Moraes, Hellen Thais Fuzii, Antonio Guilherme Maneschy Faria, Vanessa Morais Freitas, Maria Sueli da Silva Kataoka, Sérgio de Melo Alves, João de Jesus Viana Pinheiro   +7 more
doaj   +1 more source