Results 11 to 20 of about 17,041,985 (363)

Complete replication of hepatitis C virus in cell culture. [PDF]

Science, 2005
Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen.
Burton, Dennis R, Evans, Matthew J, Hynes, Richard O, Lindenbach, Brett D, Liu, Christopher C, Maruyama, Toshiaki, McKeating, Jane A, Rice, Charles M, Syder, Andrew J, Tellinghuisen, Timothy L, Wölk, Benno   +10 more
core   +2 more sources

3D Cell Culture Systems: Tumor Application, Advantages, and Disadvantages

International Journal of Molecular Sciences, 2021
The traditional two-dimensional (2D) in vitro cell culture system (on a flat support) has long been used in cancer research. However, this system cannot be fully translated into clinical trials to ideally represent physiological conditions.
O. Habanjar, M. Diab‐Assaf, F. Caldefie-Chézet, L. Delort   +3 more
semanticscholar   +1 more source

3D Cell Culture Models as Recapitulators of the Tumor Microenvironment for the Screening of Anti-Cancer Drugs

Cancers, 2021
Simple Summary Three-dimensional (3D) cell culture models have been proposed as alternatives for initial drug screening to increase drug development efficiency.
Mélanie A. G. Barbosa, C. P. Xavier, R. Pereira, V. Petrikaitė, M. Vasconcelos   +4 more
semanticscholar   +1 more source

3D cell culture models: Drug pharmacokinetics, safety assessment, and regulatory consideration

Clinical and Translational Science, 2021
Nonclinical testing has served as a foundation for evaluating potential risks and effectiveness of investigational new drugs in humans. However, the current two‐dimensional (2D) in vitro cell culture systems cannot accurately depict and simulate the rich
Hongbing Wang, Paul C. Brown, E. Chow, L. Ewart, S. Ferguson, S. Fitzpatrick, Benjamin S. Freedman, G. Guo, W. Hedrich, S. Heyward, J. Hickman, N. Isoherranen, Albert P. Li, Qi Liu, S. Mumenthaler, J. Polli, W. Proctor, A. Ribeiro, Jian-Ying Wang, R. Wange, Shiew-Mei Huang   +20 more
semanticscholar   +1 more source

Is It Time to Start Transitioning From 2D to 3D Cell Culture?

Frontiers in Molecular Biosciences, 2020
Cell culture is an important and necessary process in drug discovery, cancer research, as well as stem cell study. Most cells are currently cultured using two-dimensional (2D) methods but new and improved methods that implement three-dimensional (3D ...
Caleb Jensen, Yong Teng
semanticscholar   +1 more source

Applications of Biomaterials in 3D Cell Culture and Contributions of 3D Cell Culture to Drug Development and Basic Biomedical Research

International Journal of Molecular Sciences, 2021
The process of evaluating the efficacy and toxicity of drugs is important in the production of new drugs to treat diseases. Testing in humans is the most accurate method, but there are technical and ethical limitations.
Yujin Park, K. Huh, Sun-Woong Kang
semanticscholar   +1 more source

Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards

European Journal of Clinical Microbiology and Infectious Diseases, 2020
In a preliminary clinical study, we observed that the combination of hydroxychloroquine and azithromycin was effective against SARS-CoV-2 by shortening the duration of viral load in Covid-19 patients.
B. La Scola, M. Le Bideau, J. Andreani, V. Hoang, Clio Grimaldier, P. Colson, P. Gautret, D. Raoult   +7 more
semanticscholar   +1 more source

Stable Isotope Labeling by Amino Acids in Cell Culture, SILAC, as a Simple and Accurate Approach to Expression Proteomics*

Molecular & Cellular Proteomics, 2002
Quantitative proteomics has traditionally been performed by two-dimensional gel electrophoresis, but recently, mass spectrometric methods based on stable isotope quantitation have shown great promise for the simultaneous and automated identification and ...
Shao-En Ong, B. Blagoev, I. Kratchmarova, D. B. Kristensen, H. Steen, A. Pandey, M. Mann   +6 more
semanticscholar   +1 more source

HIF-1α Is Associated with Resistance to Hypoxia-Induced Apoptosis in Ameloblastoma

International Journal of Dentistry, 2021
Background. Ameloblastoma (AMB) is a benign odontogenic tumour, with an aggressive local behaviour and a high rate of recurrence. Previous studies have demonstrated that hypoxia-induced factor alpha 1 (HIF-1α) and activated caspase-3 contribute to tumour
Katherine Julissa Palma Valladares, Karolyny Martins Balbinot, Antonia Taiane Lopes de Moraes, Maria Sueli da Silva Kataoka, Aline Maria Pereira Cruz Ramos, Rommel Thiago Jucá Ramos, Artur Luiz da Costa da Silva, Ricardo Alves Mesquita, David Normando, Sérgio de Melo Alves Júnior, João de Jesus Viana Pinheiro   +10 more
doaj   +1 more source

Expression of Tumor Assosiated and Epithelial-mesenchymal Transition Markers in 2d and 3d Cell Cultures of Mcf-7 [PDF]

, 2016
The target effects on the expression of epithelial-mesenchymal transition regulation molecules are promising for cancer therapy, including breast cancer.
Herheliuk, T. (Tetiana), Ostapchenko, L. (Lyudmila), Perepelytsina, O. (Olena), Sydorenko, M. (Mykhailo), Yurchenko, N. (Natalia)   +4 more
core   +2 more sources