Results 11 to 20 of about 2,069,582 (340)

Molluscan cells in culture: primary cell cultures and cell lines [PDF]

open access: yesCanadian Journal of Zoology, 2013
In vitro cell culture systems from molluscs have significantly contributed to our basic understanding of complex physiological processes occurring within or between tissue-specific cells, yielding information unattainable using intact animal models.
U Bickham   +2 more
openaire   +3 more sources

T Cell Development in Culture [PDF]

open access: yesImmunity, 2002
The T cell compartment is continuously replenished by a renewable source of stem cells. In the adult, bone marrow-derived stem cells seed the thymus and initiate a developmental program that requires a series of incompletely understood signals that are normally provided by the thymus. Failure to recapitulate this process in simple in vitro cultures has
Michael J. Bevan, Sophie M. Lehar
openaire   +3 more sources

Engineered CHO cells as a novel AAV production platform for gene therapy delivery

open access: yesScientific Reports, 2023
The Herpes simplex virus (HSV)-based platform for production of recombinant adeno-associated viral vectors (rAAVs) yields higher titers and increased percentage of full capsids when compared to the triple transient transfection (TTT) method.
Abdou Nagy   +7 more
doaj   +1 more source

Angiotensin II and Angiotensin Receptors 1 and 2—Multifunctional System in Cells Biology, What Do We Know?

open access: yesCells, 2021
For years, the renin-angiotensin system (RAS) has been perceived as a system whose role is to primarily modulate the functioning of the cardiovascular system.
Maksymilian Ziaja   +3 more
doaj   +1 more source

Enzymatic basis of the Fc-selective intra-chain disulfide reduction and free thiol content variability in an antibody produced in Escherichia coli

open access: yesMicrobial Cell Factories, 2022
Background Escherichia coli (E. coli) is a promising host for production of recombinant proteins (including antibodies and antibody fragments) that don’t require complex post-translational modifications such as glycosylation.
Tomasz K. Baginski   +8 more
doaj   +1 more source

Occurrence of spontaneous polyploids in Citrus

open access: yesHorticultural Science, 2006
Seedlings recovered from underdeveloped seeds of different commercial cultivars of Citrus were studied for their ploidy level. Cytological examination revealed that maximum triploid plants were found in lime Kaghzi (15.5%) followed by grapefruit Foster ...
M. Usman   +3 more
doaj   +1 more source

Optimization of total RNA isolation method from the aromatic medicinal plant Artemisia annua L.

open access: yesInternational Journal of Plant Biology, 2012
This paper is the first report on the development of a protocol that allows rapid and simplified extraction of total RNA from Artemisia annua L., an aromatic medicinal plant.
Suganthi Appalasamy   +5 more
doaj   +1 more source

Cell isolation and culture [PDF]

open access: yesWormBook, 2013
Cell isolation and culture are essential tools for the study of cell function. Isolated cells grown under controlled conditions can be manipulated and imaged at a level of resolution that is not possible in whole animals or even tissue explants. Recent advances have allowed for large-scale isolation and culture of primary C.
Sihui Zhang, Jeffrey R. Kuhn
openaire   +3 more sources

Global Perspective of Novel Therapeutic Strategies for the Management of NeuroAIDS

open access: yesBiomolecular Concepts, 2018
Among Human immunodeficiency virus (HIV) infected individuals, around two-thirds of patients present with neuroAIDS, where HIV-associated neurocognitive disorders (HAND), and HIV-associated dementia (HAD) are the most prevailing neurological ...
Kumar Swatantra   +4 more
doaj   +1 more source

Response of letter to the editor on Procalcitonin: a promising diagnostic marker for sepsis and antibiotic therapy

open access: yesJournal of Intensive Care, 2017
In a letter to the editor, Raineri SM et al. have given an insight of another dimension of procalcitonin (PCT) use as a diagnostic tool in invasive candidiasis.
R. Saikant   +6 more
doaj   +1 more source

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